KRT82 (NM_033033) Human Tagged ORF Clone

CAT#: RC212202

KRT82 (Myc-DDK-tagged)-Human keratin 82 (KRT82)

Product Images

Circular map for RC212202

• 

  Western blot validation of overexpression lysate (Cat# LY409792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212202 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRT82
• Synonyms: Hb-2; HB2; KRTHB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212202 representing NM_033033
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTACCACTCTTTCCAGCCAGGCTCCAGGTGTGGCAGTCAGAGTTTCAGCTCATACTCGGCTGTCA
  TGCCCCGGATGGTCACCCACTATGCAGTGAGCAAGGGGCCATGCCGGCCCGGGGGTGGTAGGGGCCTCCG
  AGCTCTGGGCTGCCTTGGCTCACGGAGCCTGTGCAACGTGGGCTTTGGGAGGCCCCGGGTAGCCTCCAGG
  TGTGGAGGTACCCTGCCTGGCTTCGGGTACCGACTGGGAGCCACCTGTGGGCCTTCTGCCTGCATCACCC
  CTGTCACCATCAATGAGAGCCTGCTGGTCCCACTGGCACTGGAGATAGACCCGACTGTGCAGAGGGTAAA
  GAGGGATGAGAAGGAGCAGATCAAGTGCCTCAACAACCGTTTCGCATCTTTCATCAACAAGGTCCGTTTC
  CTGGAGCAGAAGAACAAGCTGCTGGAGACCAAGTGGAACTTCATGCAGCAGCAGAGGTGCTGCCAGACCA
  ACATCGAGCCCATCTTCGAGGGCTATATCAGCGCCCTTCGGCGGCAGCTGGACTGTGTGTCCGGGGACCG
  CGTGAGGCTAGAGTCAGAGCTCTGCAGCCTCCAGGCTGCACTGGAGGGCTACAAGAAAAAATACGAAGAG
  GAGCTCTCCCTGCGTCCCTGTGTTGAGAATGAGTTTGTTGCCTTGAAGAAGGACGTGGACACAGCCTTCC
  TGATGAAGGCTGACCTGGAGACCAACGCAGAGGCACTCGTGCAGGAGATCGACTTCCTGAAAAGCCTGTA
  TGAGGAGGAGATCTGCCTGCTCCAGTCTCAGATCTCTGAGACCTCGGTCATTGTGAAGATGGACAACAGC
  CGGGAGCTGGACGTGGACGGCATCATCGCTGAGATCAAGGCGCAGTATGACGACATCGCCAGCCGCAGCA
  AAGCCGAAGCAGAGGCCTGGTACCAGTGCCGGTATGAGGAGCTGAGAGTCACAGCTGGGAACCACTGTGA
  CAACCTCCGCAACCGTAAGAACGAGATCCTGGAAATGAATAAACTGATCCAGCGGCTGCAGCAAGAAACC
  GAGAATGTCAAAGCCCAGCGCTGCAAACTTGAGGGTGCCATAGCTGAGGCAGAGCAGCAGGGCGAGGCGG
  CTCTCAATGATGCCAAGTGCAAGCTGGCAGGGCTGGAGGAGGCTCTGCAGAAGGCCAAGCAGGACATGGC
  CTGCCTGCTCAAGGAATATCAGGAGGTGATGAACTCCAAGCTGGGCCTGGACATCGAGATCGCCACCTAC
  AGGCGCCTGCTGGAGGGTGAAGAGCACAGGCTGTGCGAAGGCATCGGGCCCGTGAATATCTCAGTGAGCA
  GCTCCAAAGGCGCCTTCCTGTACGAGCCATGTGGGGTCAGCACGCCTGTCCTCAGCACTGGCGTCCTCAG
  GAGCAATGGGGGCTGCAGCATCGTGGGCACTGGTGAACTCTATGTCCCCTGCGAGCCCCAGGGGCTACTG
  AGCTGTGGGAGCGGGCGGAAATCCAGCATGACGCTAGGAGCTGGGGGCAGCTCCCCCAGCCACAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212202 representing NM_033033
  Red=Cloning site Green=Tags(s)
  
  MSYHSFQPGSRCGSQSFSSYSAVMPRMVTHYAVSKGPCRPGGGRGLRALGCLGSRSLCNVGFGRPRVASR
  CGGTLPGFGYRLGATCGPSACITPVTINESLLVPLALEIDPTVQRVKRDEKEQIKCLNNRFASFINKVRF
  LEQKNKLLETKWNFMQQQRCCQTNIEPIFEGYISALRRQLDCVSGDRVRLESELCSLQAALEGYKKKYEE
  ELSLRPCVENEFVALKKDVDTAFLMKADLETNAEALVQEIDFLKSLYEEEICLLQSQISETSVIVKMDNS
  RELDVDGIIAEIKAQYDDIASRSKAEAEAWYQCRYEELRVTAGNHCDNLRNRKNEILEMNKLIQRLQQET
  ENVKAQRCKLEGAIAEAEQQGEAALNDAKCKLAGLEEALQKAKQDMACLLKEYQEVMNSKLGLDIEIATY
  RRLLEGEEHRLCEGIGPVNISVSSSKGAFLYEPCGVSTPVLSTGVLRSNGGCSIVGTGELYVPCEPQGLL
  SCGSGRKSSMTLGAGGSSPSHKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033033
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033033.4
• RefSeq Size: 2681 bp
• RefSeq ORF: 1542 bp
• Locus ID: 3888
• UniProt ID: Q9NSB4
• Cytogenetics: 12q13.13
• MW: 56.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this keratin appears to be a hair cuticle-specific keratin. [provided by RefSeq, Jul 2008]