SMUG1 (NM_014311) Human Tagged ORF Clone
CAT#: RC212141
SMUG1 (Myc-DDK-tagged)-Human single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_014311" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SMUG1 |
Synonyms | FDG; HMUDG; UNG3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212141 representing NM_014311
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCCCAGCCCTGCC CTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCTGAGCTGAGCCAGCTGCAGTT TTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTATGCATGGGAGCCACATCGCAACTACGTGACT CGCTACTGCCAGGGCCCCAAGGAAGTACTCTTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGA CTGGGGTGCCCTTTGGGGAAGTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCC TCCCCAAGAGCATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGTGAGTGGTGCCCGA TTCTGGGGCTTTTTCCGGAACCTCTGTGGACAGCCTGAGGTCTTCTTCCATCACTGTTTTGTCCACAATC TATGCCCTCTGCTTTTCCTGGCTCCCAGCGGGCGCAACCTTACTCCTGCTGAGCTGCCTGCCAAGCAGCG AGAACAGCTTCTTGGGATCTGTGATGCAGCCCTCTGCCGGCAGGTGCAGCTGCTGGGGGTGCGGCTGGTG GTGGGAGTTGGGCGACTGGCAGAGCAGCGGGCACGACGGGCTCTGGCAGGCCTGATGCCAGAGGTCCAGG TGGAAGGGCTCCTGCATCCCTCTCCCCGTAACCCACAGGCCAACAAGGGCTGGGAGGCAGTGGCCAAGGA AAGATTGAATGAGCTGGGGCTGCTGCCACTGCTGTTGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212141 representing NM_014311
Red=Cloning site Green=Tags(s) MPQAFLLGSIHEPAGALMEPQPCPGSLAESFLEEELRLNAELSQLQFSEPVGIIYNPVEYAWEPHRNYVT RYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVSMVRDWLGIVGPVLTPPQEHPKRPVLGLECPQSEVSGAR FWGFFRNLCGQPEVFFHHCFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLGICDAALCRQVQLLGVRLV VGVGRLAEQRARRALAGLMPEVQVEGLLHPSPRNPQANKGWEAVAKERLNELGLLPLLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014311 |
ORF Size | 810 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014311.2 |
RefSeq Size | 1570 bp |
RefSeq ORF | 813 bp |
Locus ID | 23583 |
UniProt ID | Q53HV7 |
Cytogenetics | 12q13.13 |
Domains | UDG |
Protein Families | Druggable Genome |
Protein Pathways | Base excision repair |
MW | 29.7 kDa |
Gene Summary | This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212141L3 | Lenti ORF clone of Human single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1), Myc-DDK-tagged |
USD 600.00 |
|
RC212141L4 | Lenti ORF clone of Human single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1), mGFP tagged |
USD 600.00 |
|
RG212141 | SMUG1 (tGFP-tagged) - Human single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) |
USD 500.00 |
|
SC115074 | SMUG1 (untagged)-Human single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) |
USD 300.00 |
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