RAD50 (NM_005732) Human Tagged Lenti ORF Clone
CAT#: RC211751L2
- LentiORF®
Lenti-ORF clone of RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50)
"NM_005732" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | RAD50 |
Synonyms | hRad50; NBSLD; RAD502 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211751).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005732 |
ORF Size | 3936 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005732.3 |
RefSeq Size | 6597 bp |
RefSeq ORF | 3939 bp |
Locus ID | 10111 |
UniProt ID | Q92878 |
Cytogenetics | 5q31.1 |
Domains | Rad50_zn_hook |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination, Non-homologous end-joining |
MW | 154.3 kDa |
Gene Summary | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211751 | RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 1,713.00 |
|
RC211751L1 | Lenti-ORF clone of RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 2,013.00 |
|
RC211751L3 | Lenti-ORF clone of RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 2,013.00 |
|
RC211751L4 | Lenti-ORF clone of RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 2,013.00 |
|
RG211751 | RAD50 (tGFP-tagged) - Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 1,913.00 |
|
SC110122 | RAD50 (untagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
USD 1,714.00 |
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