PRDM5 (NM_018699) Human Tagged ORF Clone

CAT#: RC211714

PRDM5 (Myc-DDK-tagged)-Human PR domain containing 5 (PRDM5)

Product Images

Circular map for RC211714

• 

  Western blot validation of overexpression lysate (Cat# LY412894) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211714 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRDM5
• Synonyms: BCS2; PFM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211714 representing NM_018699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCATGTACGTGCCGGACAGGTTCTCCCTGAAGTCCTCCCGGGTTCAGGACGGCATGGGGCTCT
  ACACGGCCCGCAGAGTGCGAAAGGGTGAAAAGTTCGGACCCTTTGCTGGAGAGAAGAGAATGCCTGAAGA
  CTTGGATGAAAATATGGATTACAGGTTGATGTGGGAGGTTCGTGGGAGTAAGGGAGAAGTTTTGTACATT
  TTGGATGCTACCAACCCACGGCACTCCAACTGGCTTCGCTTCGTTCATGAGGCACCATCTCAGGAGCAGA
  AGAACTTGGCTGCCATTCAAGAAGGAGAAAACATTTTCTATTTGGCAGTTGAAGATATAGAAACAGACAC
  GGAGCTTCTGATTGGCTACCTGGATAGTGACATGGAGGCTGAGGAGGAAGAACAGCAAATTATGACAGTC
  ATCAAAGAAGGGGAAGTTGAAAATTCTAGAAGACAATCAACAGCGGGCAGAAAAGATCGCCTTGGCTGTA
  AAGAGGACTATGCTTGTCCTCAATGTGAATCGAGTTTTACCAGTGAGGATATTCTTGCTGAGCATCTCCA
  GACATTGCACCAGAAACCCACAGAGGAGAAAGAATTTAAGTGCAAGAACTGTGGGAAGAAATTCCCAGTT
  AAGCAGGCTTTGCAAAGACATGTTCTTCAGTGCACAGCGAAAAGCAGTCTAAAGGAGTCTTCGCGAAGTT
  TTCAGTGCTCTGTTTGCAATTCTTCCTTCAGTTCAGCATCGAGTTTTGAGCAGCACCAGGAGACTTGCCG
  GGGGGATGCCAGGTTTGTGTGCAAGGCTGACAGCTGTGGAAAGAGGCTGAAGAGCAAGGATGCCCTGAAA
  AGACACCAGGAAAATGTCCACACTGGAGATCCTAAGAAAAAGCTTATATGTTCAGTGTGCAATAAAAAGT
  GTTCTTCAGCATCAAGCCTACAGGAACATAGAAAGATTCATGAGATATTTGATTGTCAAGAATGTATGAA
  GAAATTTATTTCAGCTAATCAGCTAAAACGTCATATGATCACCCACTCAGAAAAACGACCCTATAATTGC
  GAGATTTGTAATAAGTCTTTCAAGAGGCTTGATCAAGTGGGTGCTCACAAAGTAATACACAGCGAAGACA
  AACCTTACAAATGCAAACTTTGTGGAAAGGGATTTGCCCACAGAAATGTTTACAAGAATCATAAGAAGAC
  CCACTCTGAGGAGAGACCGTTCCAATGTGAAGAATGTAAAGCTTTGTTCCGGACCCCATTTTCTTTACAG
  AGACACCTGCTAATACATAACAGTGAGAGGACTTTCAAGTGCCATCACTGCGATGCTACCTTTAAGAGGA
  AGGATACATTAAATGTTCATGTCCAGGTGGTTCATGAAAGACACAAGAAGTATAGGTGTGAGCTATGTAA
  TAAGGCCTTTGTTACACCTTCAGTGCTTAGAAGTCATAAGAAAACACATACAGGAGAAAAGGAGAAAATC
  TGTCCATATTGTGGCCAGAAATTTGCCAGCAGTGGTACACTCAGAGTTCATATCCGGAGCCACACAGGTG
  AGCGTCCCTATCAATGTCCTTACTGTGAAAAAGGATTCAGTAAAAATGATGGACTGAAGATGCACATTCG
  TACTCACACCAGGGAGAAGCCGTACAAGTGCTCAGAGTGCAGCAAGGCCTTCAGCCAGAAGCGAGGCCTG
  GATGAGCACAAGAGGACGCACACTGGAGAAAAGCCTTTTCAGTGTGATGTTTGTGATTTGGCTTTTAGCC
  TGAAGAAAATGCTGATTCGACACAAGATGACTCATAATCCCAATCGTCCCCTGGCAGAATGCCAGTTTTG
  CCATAAGAAGTTTACAAGGAATGACTACCTCAAAGTGCACATGGACAATATCCATGGTGTAGCTGACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211714 representing NM_018699
  Red=Cloning site Green=Tags(s)
  
  MLGMYVPDRFSLKSSRVQDGMGLYTARRVRKGEKFGPFAGEKRMPEDLDENMDYRLMWEVRGSKGEVLYI
  LDATNPRHSNWLRFVHEAPSQEQKNLAAIQEGENIFYLAVEDIETDTELLIGYLDSDMEAEEEEQQIMTV
  IKEGEVENSRRQSTAGRKDRLGCKEDYACPQCESSFTSEDILAEHLQTLHQKPTEEKEFKCKNCGKKFPV
  KQALQRHVLQCTAKSSLKESSRSFQCSVCNSSFSSASSFEQHQETCRGDARFVCKADSCGKRLKSKDALK
  RHQENVHTGDPKKKLICSVCNKKCSSASSLQEHRKIHEIFDCQECMKKFISANQLKRHMITHSEKRPYNC
  EICNKSFKRLDQVGAHKVIHSEDKPYKCKLCGKGFAHRNVYKNHKKTHSEERPFQCEECKALFRTPFSLQ
  RHLLIHNSERTFKCHHCDATFKRKDTLNVHVQVVHERHKKYRCELCNKAFVTPSVLRSHKKTHTGEKEKI
  CPYCGQKFASSGTLRVHIRSHTGERPYQCPYCEKGFSKNDGLKMHIRTHTREKPYKCSECSKAFSQKRGL
  DEHKRTHTGEKPFQCDVCDLAFSLKKMLIRHKMTHNPNRPLAECQFCHKKFTRNDYLKVHMDNIHGVADS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018699
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018699.4
• RefSeq Size: 2492 bp
• RefSeq ORF: 1893 bp
• Locus ID: 11107
• UniProt ID: Q9NQX1
• Cytogenetics: 4q27
• Domains: SET, zf-C2H2
• Protein Families: Transcription Factors
• MW: 72.9 kDa
• Gene Summary: The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. [provided by RefSeq, Jul 2008]