KRIT1 (NM_004912) Human Tagged ORF Clone

CAT#: RC211686

KRIT1 (Myc-DDK-tagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 2

Product Images

Circular map for RC211686

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRIT1
• Synonyms: CAM; CCM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211686 representing NM_004912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATCCAGAAAACATAGAAGATGCATATGTTGCTGTTATTCGTCCAAAGAATACTGCCAGTCTCA
  ATTCTCGGGAATACAGAGCTAAGTCATATGAAATTTTGTTGCATGAAGTTCCCATTGAAGGACAGAAAAA
  AAAGAGAAAGAAAGTTTTATTGGAAACGAAACTTCAAGGCAACAGTGAAATAACACAAGGCATATTGGAT
  TACGTAGTAGAAACCACCAAACCAATTTCTCCTGCAAACCAGGGTATCAGAGGAAAACGAGTTGTACTAA
  TGAAAAAATTTCCTCTGGATGGAGAGAAGATGGGCAGAGAAGCATCATTATTTATTGTTCCATCAGTTGT
  CAAAGATAATACTAAATACACATATACCCCAGGATGCCCAATTTTTTACTGCTTACAAGATATTATGCGA
  GTCTGTAGTGAATCCAGTACTCATTTTGCTACACTTACAGCAAGGATGTTAATAGCCTTGGATAAGTGGT
  TAGATGAACGTCATGCACAATCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCTCTTGAGCGGATAAA
  AACTAATGTCATAAATCCTGCATATGCTACTGAATCAGGTCAGACAGAAAACTCACTACATATGGGCTAT
  AGTGCACTAGAAATAAAGAGTAAAATGTTAGCCCTAGAGAAAGCAGATACCTGTATTTACAACCCTTTGT
  TTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTTGGTCTAGGAGC
  TCCAGACTACTCAAAAATCCAAATACCTAAACAGGAAAAATGGCAGAGAAGCATGAGCAGTGTCACAGAA
  GACAAGGAACGACAGTGGGTAGATGATTTTCCTCTCCACCGAAGCGCCTGTGAAGGAGATTCAGAATTAC
  TAAGCCGTCTTCTCAGTGAAAGATTTTCAGTCAACCAGTTAGATAGTGACCACTGGGCACCCATTCATTA
  TGCATGCTGGTATGGAAAAGTTGAGGCCACTCGCATATTGTTAGAGAAAGGAAAGTGCAATCCAAACCTT
  TTAAATGGACAACTTAGTTCTCCTCTTCATTTTGCTGCTGGAGGAGGACATGCTGAAATAGTACAGATTC
  TCCTAAACCACCCAGAAACGGATAGACATATAACAGACCAACAAGGAAGATCTCCATTAAATATTTGTGA
  AGAAAACAAACAAAACAACTGGGAAGAAGCTGCAAAATTGTTGAAGGAAGCAATTAACAAACCATATGAA
  AAAGTTCGAATATACAGAATGGATGGGTCATATCGTTCTGTTGAATTGAAGCATGGAAATAATACCACAG
  TGCAGCAGATAATGGAAGGAATGCGTCTCTCTCAAGAAACTCAGCAATATTTCACTATATGGATTTGTTC
  AGAAAACCTCAGCCTTCAACTCAAACCATATCATAAACCCTTGCAACATGTTCGTGACTGGCCAGAAATA
  CTTGCTGAATTGACTAATCTGGATCCTCAAAGGGAAACACCTCAGCTTTTTCTAAGAAGAGATGTGAGAC
  TTCCCTTGGAAGTTGAAAAACAGATTGAAGACCCACTAGCTATTCTTATTCTCTTTGATGAAGCCAGATA
  TAATTTATTGAAGGGCTTTTATACAGCTCCTGATGCTAAGCTGATAACATTGGCAAGTCTGCTTTTGCAA
  ATAGTCTATGGAAATTATGAGAGTAAAAAACACAAGCAAGGTTTCCTAAATGAAGAAAATCTAAAATCCA
  TCGTACCTGTTACCAAACTGAAAAGTAAGGCACCTCACTGGACAAATCGCATACTTCATGAATACAAGAA
  TCTCAGTACAAGTGAAGGTGTCAGTAAAGAAATGCATCACCTTCAGCGCATGTTCTTACAGAATTGCTGG
  GAAATTCCTACTTATGGAGCAGCATTTTTCACAGGACAGATATTTACAAAGGCAAGCCCCAGCAATCATA
  AAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTCCTCAACATGGAAACTAAGGCTTT
  ACTCATCAGTCTTAAGTATGGTTGTTTTATGTGGCAATTGGGAGATACTGATACTTGTTTTCAGATCCAT
  AGCATGGAAAATAAAATGAGCTTTATAGTACATACAAAACAGGCTGGTCTCGTGGTAAAACTGTTAATGA
  AGCTAAATGGACAGTTAATGCCCACTGAAAGAAATTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211686 representing NM_004912
  Red=Cloning site Green=Tags(s)
  
  MGNPENIEDAYVAVIRPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRKKVLLETKLQGNSEITQGILD
  YVVETTKPISPANQGIRGKRVVLMKKFPLDGEKMGREASLFIVPSVVKDNTKYTYTPGCPIFYCLQDIMR
  VCSESSTHFATLTARMLIALDKWLDERHAQSHFIPALFRPSPLERIKTNVINPAYATESGQTENSLHMGY
  SALEIKSKMLALEKADTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDYSKIQIPKQEKWQRSMSSVTE
  DKERQWVDDFPLHRSACEGDSELLSRLLSERFSVNQLDSDHWAPIHYACWYGKVEATRILLEKGKCNPNL
  LNGQLSSPLHFAAGGGHAEIVQILLNHPETDRHITDQQGRSPLNICEENKQNNWEEAAKLLKEAINKPYE
  KVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHVRDWPEI
  LAELTNLDPQRETPQLFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQ
  IVYGNYESKKHKQGFLNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLSTSEGVSKEMHHLQRMFLQNCW
  EIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYGCFMWQLGDTDTCFQIH
  SMENKMSFIVHTKQAGLVVKLLMKLNGQLMPTERNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004912
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004912.4
• RefSeq Size: 4870 bp
• RefSeq ORF: 2211 bp
• Locus ID: 889
• UniProt ID: O00522
• Cytogenetics: 7q21.2
• Domains: B41, ANK
• Protein Families: Druggable Genome
• MW: 84.3 kDa
• Gene Summary: This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]