nkx6.2 (NKX6-2) (NM_177400) Human Tagged ORF Clone
CAT#: RC210963
NKX6 (Myc-DDK-tagged)-Human NK6 homeobox 2 (NKX6-2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_177400" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | nkx6.2 |
Synonyms | GTX; NKX6.2; NKX6B; SPAX8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210963 representing NM_177400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACTAACCGCCCGGGCGCGTTCGTGCTGAGCAGTGCCCCGCTGGCCGCGCTGCACAACATGGCCG AGATGAAGACGTCGCTGTTCCCCTACGCGCTGCAGGGTCCGGCCGGCTTCAAGGCGCCCGCGCTGGGGGG CCTGGGCGCGCAGCTCCCGCTCGGGACCCCGCACGGCATCAGCGACATCCTGGGCCGGCCCGTGGGCGCG GCGGGCGGGGGCCTCCTGGGGGGGCTGCCCCGGCTCAACGGGCTCGCGTCGTCCGCCGGCGTTTACTTCG GGCCCGCGGCCGCTGTGGCGCGCGGCTACCCCAAGCCCCTGGCCGAGCTGCCGGGGCGCCCGCCCATCTT CTGGCCCGGCGTGGTGCAGGGCGCGCCCTGGAGGGACCCGCGTCTGGCTGGCCCGGCCCCGGCCGGCGGC GTCCTGGACAAGGACGGGAAGAAGAAGCACTCGCGCCCGACCTTCTCGGGCCAGCAGATCTTCGCGCTGG AGAAAACCTTCGAGCAGACCAAGTACCTGGCGGGCCCGGAGCGCGCGCGTCTCGCCTACTCGCTGGGCAT GACCGAGAGCCAGGTGAAGGTCTGGTTCCAGAACCGCCGGACCAAGTGGCGCAAGCGGCACGCGGCGGAG ATGGCGTCGGCCAAGAAGAAGCAGGACTCGGACGCCGAGAAGCTGAAGGTGGGCGGCTCGGACGCGGAGG ACGACGACGAATACAACCGGCCCCTGGACCCCAACTCGGACGACGAGAAGATCACGCGGCTGCTCAAGAA GCACAAACCCTCGAACTTGGCGCTGGTCAGCCCGTGCGGCGGCGGCGCGGGGGACGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210963 representing NM_177400
Red=Cloning site Green=Tags(s) MDTNRPGAFVLSSAPLAALHNMAEMKTSLFPYALQGPAGFKAPALGGLGAQLPLGTPHGISDILGRPVGA AGGGLLGGLPRLNGLASSAGVYFGPAAAVARGYPKPLAELPGRPPIFWPGVVQGAPWRDPRLAGPAPAGG VLDKDGKKKHSRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKRHAAE MASAKKKQDSDAEKLKVGGSDAEDDDEYNRPLDPNSDDEKITRLLKKHKPSNLALVSPCGGGAGDAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_177400 |
ORF Size | 831 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_177400.2 |
RefSeq Size | 834 bp |
RefSeq ORF | 834 bp |
Locus ID | 84504 |
UniProt ID | Q9C056 |
Cytogenetics | 10q26.3 |
Protein Families | Transcription Factors |
MW | 29.1 kDa |
Gene Summary | Transcription factor with repressor activity involved in the regulation of axon-glial interactions at myelin paranodes in oligodendrocytes. Binds to the consensus DNA sequence 5'-(A/T)TTAATGA-3'. In oligodendrocytes, binds to MBP and PLP1 promoter regions.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210963L1 | Lenti ORF clone of Human NK6 homeobox 2 (NKX6-2), Myc-DDK-tagged |
USD 750.00 |
|
RC210963L2 | Lenti ORF clone of Human NK6 homeobox 2 (NKX6-2), mGFP tagged |
USD 750.00 |
|
RC210963L3 | Lenti ORF clone of Human NK6 homeobox 2 (NKX6-2), Myc-DDK-tagged |
USD 750.00 |
|
RC210963L4 | Lenti ORF clone of Human NK6 homeobox 2 (NKX6-2), mGFP tagged |
USD 750.00 |
|
RG210963 | NKX6 (tGFP-tagged) - Human NK6 homeobox 2 (NKX6-2) |
USD 650.00 |
|
SC307088 | NKX6 (untagged)-Human NK6 homeobox 2 (NKX6-2) |
USD 450.00 |
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