LRRC41 (NM_006369) Human Tagged ORF Clone

CAT#: RC210927

LRRC41 (Myc-DDK-tagged)-Human leucine rich repeat containing 41 (LRRC41)

Product Images

Circular map for RC210927

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  Western blot validation of overexpression lysate (Cat# LY416688) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210927 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LRRC41 protein (Cat# TP310927). The protein was produced from HEK293T cells transfected with LRRC41 cDNA clone (Cat# RC210927) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC41
• Synonyms: MUF1; PP7759
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210927 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCGAGGCCTGGCGCGCCCGGAGTTGCTGGTTCTGTGAGGTAGCGGCGGCAACGACCATGG
  AGGCCACGTCCCGGGAGGCGGCGCCAGCGAAGAGCTCGGCCTCGGGCCCCAACGCTCCCCCCGCCCTGTT
  CGAGCTGTGCGGGCGGGCGGTGAGCGCCCATATGGGGGTTCTGGAGAGCGGGGTGTGGGCCCTCCCAGGC
  CCAATACTTCAAAGCATCCTACCTCTGCTCAATATATATTACTTGGAGAGGATTGAGGAAACTGCCCTCA
  AGAAAGGCCTCTCAACTCAGGCCATCTGGCGCCGACTCTGGGATGAACTGATGAAGACAAGGCCTTCCAG
  TTTGGAAAGTGTGACATGTTGGCGAGCCAAGTTTATGGAGGCCTTTTTTTCCCATGTTCTACGTGGGACC
  ATTGATGTGTCTTCTGACAGGCGTCTTTGTGATCAGCGGTTCTCACCTCTTCTGCACAGCTCCCGCCATG
  TCCGACAGCTCACCATCTGTAACATGCTGCAGGGTGCAACCGAGCTGGTGGCTGAGCCCAACCGCAGGGT
  TCTGGAGACCCTGGCCAGCTCCCTGCACACTCTCAAGTTCCGCCACCTGCTGTTCTCTGATGTGGCTGCT
  CAGCAGTCACTTCGGCAGCTGTTGCATCAGCTCATTCACCATGGGGCTGTCAGTCAAGTGTCGCTATACT
  CCTGGCCTGTGCCTGAGTCAGCCCTTTTCATCCTTATTCTCACCATGAGTGCTGGCTTCTGGCAACCAGG
  GCCTGGTGGCCCACCCTGCCGCCTCTGTGGAGAGGCCTCCCGAGGCCGGGCCCCATCCCGAGATGAAGGG
  TCCCTCTTATTGGGCTCACGTCGGCCCCGCCGGGATGCTGCTGAGCGATGTGCTGCAGCCCTGATGGCCA
  GCCGGCGTAAGAGTGAAGCCAAGCAGATGCCCAGAGCTGCACCTGCCACTCGGGTAACACGCCGGAGCAC
  ACAGGAGAGCCTGACAGCAGGCGGAACAGACCTTAAGAGGGAGCTGCACCCCCCAGCCACCTCCCATGAG
  GCTCCTGGCACCAAGCGGTCACCTTCTGCTCCAGCAGCCACCTCCTCTGCCTCTTCTTCTACATCCTCAT
  ACAAACGGGCACCAGCTAGCTCAGCCCCACAGCCTAAGCCCCTAAAGCGTTTCAAGCGAGCTGCAGGGAA
  GAAGGGTGCTCGCACCCGTCAGGGGCCTGGTGCAGAGTCTGAAGACCTGTATGACTTCGTTTTTATTGTG
  GCTGGCGAGAAGGAGGATGGCGAAGAGATGGAGATTGGGGAAGTGGCTTGTGGAGCTTTGGATGGATCAG
  ATCCCAGCTGCCTGGGGCTTCCAGCACTGGAAGCTTCACAAAGATTCCGCAGCATCTCCACCTTGGAGCT
  ATTCACAGTTCCACTCTCCACAGAGGCAGCCCTGACACTATGCCACCTGCTGAGCTCCTGGGTGTCACTG
  GAGAGCCTCACACTCTCCTACAATGGCCTGGGCTCTAACATCTTCCGCCTGCTAGACAGCCTGCGGGCCC
  TGTCAGGCCAGGCTGGATGTCGCCTCCGTGCCCTGCATCTCAGTGACCTGTTCTCACCACTGCCCATCCT
  GGAGCTGACACGTGCTATCGTGCGAGCACTGCCCCTGCTACGGGTCCTCTCTATTCGTGTTGACCACCCA
  AGCCAGCGGGACAACCCTGGTGTGCCAGGGAATGCAGGGCCCCCTAGCCACATAATAGGCGATGAGGAGA
  TACCAGAAAACTGCCTGGAGCAGTTGGAGATGGGATTTCCACGGGGAGCCCAGCCAGCCCCACTGCTGTG
  CTCCATTCTGAAGGCCTCGGGTTCTCTGCAGCAGCTGTCCCTGGATAGTGCCACCTTTGCCTCTCCCCAG
  GATTTTGGGCTTGTTTTGCAAACACTCAAAGAGTACAACCTAGCCCTGAAAAGACTGAGCTTCCATGACA
  TGAATCTCGCTGACTGTCAGAGCGAGGTGCTCTTTTTGCTACAGAATCTGACTCTGCAAGAGATTACCTT
  CTCCTTCTGCCGTCTGTTTGAGAAGCGCCCAGCCCAATTTCTGCCTGAGATGGTTGCTGCTATGAAGGGC
  AACTCCACACTGAAGGGCCTCCGGCTGCCAGGGAACCGCCTGGGGAATGCTGGCCTGCTGGCCTTGGCAG
  ATGTTTTCTCAGAGGATTCATCCTCCTCTCTCTGTCAGCTGGACATCAGTTCCAACTGCATCAAGCCAGA
  TGGGCTTCTGGAGTTCGCCAAGCGGCTGGAGCGCTGGGGCCGTGGAGCCTTTGGTCACCTGCGCCTCTTC
  CAAAACTGGCTGGACCAGGATGCAGTCACAGCCAGGGAAGCCATCCGGCGGCTCCGGGCTACCTGCCATG
  TGGTTAGCGACTCATGGGACTCATCCCAGGCCTTCGCAGATTATGTTAGCACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210927 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPEAWRARSCWFCEVAAATTMEATSREAAPAKSSASGPNAPPALFELCGRAVSAHMGVLESGVWALPG
  PILQSILPLLNIYYLERIEETALKKGLSTQAIWRRLWDELMKTRPSSLESVTCWRAKFMEAFFSHVLRGT
  IDVSSDRRLCDQRFSPLLHSSRHVRQLTICNMLQGATELVAEPNRRVLETLASSLHTLKFRHLLFSDVAA
  QQSLRQLLHQLIHHGAVSQVSLYSWPVPESALFILILTMSAGFWQPGPGGPPCRLCGEASRGRAPSRDEG
  SLLLGSRRPRRDAAERCAAALMASRRKSEAKQMPRAAPATRVTRRSTQESLTAGGTDLKRELHPPATSHE
  APGTKRSPSAPAATSSASSSTSSYKRAPASSAPQPKPLKRFKRAAGKKGARTRQGPGAESEDLYDFVFIV
  AGEKEDGEEMEIGEVACGALDGSDPSCLGLPALEASQRFRSISTLELFTVPLSTEAALTLCHLLSSWVSL
  ESLTLSYNGLGSNIFRLLDSLRALSGQAGCRLRALHLSDLFSPLPILELTRAIVRALPLLRVLSIRVDHP
  SQRDNPGVPGNAGPPSHIIGDEEIPENCLEQLEMGFPRGAQPAPLLCSILKASGSLQQLSLDSATFASPQ
  DFGLVLQTLKEYNLALKRLSFHDMNLADCQSEVLFLLQNLTLQEITFSFCRLFEKRPAQFLPEMVAAMKG
  NSTLKGLRLPGNRLGNAGLLALADVFSEDSSSSLCQLDISSNCIKPDGLLEFAKRLERWGRGAFGHLRLF
  QNWLDQDAVTAREAIRRLRATCHVVSDSWDSSQAFADYVSTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006369
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006369.4, NP_006360.3
• RefSeq Size: 2960 bp
• RefSeq ORF: 2439 bp
• Locus ID: 10489
• UniProt ID: Q15345
• Cytogenetics: 1p34.1-p33
• Domains: LRR_RI
• MW: 88.7 kDa
• Gene Summary: Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]