MAGEE2 (NM_138703) Human Tagged ORF Clone

CAT#: RC210895

MAGEE2 (Myc-DDK-tagged)-Human melanoma antigen family E, 2 (MAGEE2)

Product Images

Circular map for RC210895

• 

  Western blot validation of overexpression lysate (Cat# LY408553) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210895 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGEE2
• Synonyms: HCA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210895 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGGTAAGCCAGAATGCACGCCACTGTAGCGCAGAGATCACTGCAGATTACGGCGACGGCAGAG
  GTGAAATACAAGCTACTAACGCCTCCGGGTCCCCCACCTCCATGCTAGTCGTTGATGCCCCCCAGTGCCC
  TCAGGCGCCAATCAACTCTCAGTGTGTCAACACTTCCCAGGCCGTTCAGGACCCGAATGACCTGGAGGTC
  CTGATCGACGAGCAGTCCAGACGTTTGGGGGCGCTCAGGGTCCATGACCCTCTAGAAGACAGGTCGATTG
  CTTTGGTGAATTTCATGAGAATGAAAAGCCAGACGGAGGGGTCTATCCAGCAGTCCGAGATGCTGGAGTT
  TCTCAGAGAGTACTCAGATCAGTTCCCTGAGATCCTCAGACGAGCCTCAGCTCACCTGGACCAGGTCTTT
  GGGTTGAACCTGAGAGTTATTGATCCTCAGGCTGACACCTACAATTTAGTCAGCAAACGGGGTTTCCAGA
  TCACCGATAGGATAGCGGAGTCCCTGGACATGCCAAAAGCAAGTCTCCTGGCCCTAGTCCTAGGCCACAT
  CCTCTTGAATGGGAACCGAGCAAGAGAGGCCTCCATTTGGGACTTGCTGCTAAAAGTTGATATGTGGGAT
  AAGCCTCAGAGGATCAACAACCTCTTTGGGAACACAAGGAACCTCCTCACTACTGACTTTGTGTGCATGC
  GATTCTTGGAGTACTGGCCGGTGTATGGCACTAATCCCCTTGAGTTTGAGTTCTTGTGGGGCTCTAGAGC
  CCACAGGGAAATCACAAAGATGGAAGCCCTGAAGTTTGTGTCAGATGCCCATGATGAAGAACCCTGGAGC
  TGGCCAGAAGAATATAATAAGGCCCTGGAAGGTGACAAAACCAAAGAAAGAAGCCTGACTGCTGGCTTAG
  AGTTCTGGTCGGAGGACACTATGAATGATAAGGCAAATGATTTGGTCCAGTTGGCTATTAGTGTCACTGA
  GGAGATGCTGCCTATACATCAGGATGAGCTATTGGCTCACACTGGCAAAGAATTTGAGGATGTGTTCCCA
  AATATCCTCAATAGAGCTACTCTAATTCTTGATATGTTCTATGGGTTGTCTCTGATTGAGGTTGATACCA
  GTGAGCACATCTACCTCCTTGTCCAGCAACCAGAATCAGAGGAAGAGCAAGTGATGCTAGAGAGCCTGGG
  GAGACCCACTCAAGAATATGTAATGCCAATCCTAGGTTTGATCTTCCTGATGGGCAACCGTGTCAAAGAG
  GCCAATGTCTGGAACTTGCTTCGAAGATTTAGTGTGGATGTAGGGAGAAAGCATTCCATCACCCGTAAGC
  TTATGAGACAGCGCTATCTGGAATGCAGGCCACTGTCCTACTCTAATCCAGTTGAATATGAGCTTCTATG
  GGGTCCTCGAGCTCACCATGAAACCATCAAAATGAAAGTCTTGGAGTACATGGCCAGGCTCTACAGAAAG
  CGACCACAGAACTGGCCAGAACAATATAGGGAGGCTGTGGAAGATGAGGAGGCCAGAGCCAAATCTGAGG
  CAACTATCATGTTTTTCCTTGACCCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210895 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLVSQNARHCSAEITADYGDGRGEIQATNASGSPTSMLVVDAPQCPQAPINSQCVNTSQAVQDPNDLEV
  LIDEQSRRLGALRVHDPLEDRSIALVNFMRMKSQTEGSIQQSEMLEFLREYSDQFPEILRRASAHLDQVF
  GLNLRVIDPQADTYNLVSKRGFQITDRIAESLDMPKASLLALVLGHILLNGNRAREASIWDLLLKVDMWD
  KPQRINNLFGNTRNLLTTDFVCMRFLEYWPVYGTNPLEFEFLWGSRAHREITKMEALKFVSDAHDEEPWS
  WPEEYNKALEGDKTKERSLTAGLEFWSEDTMNDKANDLVQLAISVTEEMLPIHQDELLAHTGKEFEDVFP
  NILNRATLILDMFYGLSLIEVDTSEHIYLLVQQPESEEEQVMLESLGRPTQEYVMPILGLIFLMGNRVKE
  ANVWNLLRRFSVDVGRKHSITRKLMRQRYLECRPLSYSNPVEYELLWGPRAHHETIKMKVLEYMARLYRK
  RPQNWPEQYREAVEDEEARAKSEATIMFFLDPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138703
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138703.5
• RefSeq Size: 2257 bp
• RefSeq ORF: 1572 bp
• Locus ID: 139599
• UniProt ID: Q8TD90
• Cytogenetics: Xq13.3
• MW: 60.4 kDa
• Gene Summary: This gene encodes a member of the E subfamily of MAGE (melanoma antigen-encoding gene) gene family. The gene is intronless and the encoded protein has two of the MAGE domains which are characteristic of MAGE family proteins. [provided by RefSeq, Sep 2011]