Thyroid Peroxidase (TPO) (NM_000547) Human Tagged Lenti ORF Clone
CAT#: RC210659L4
- LentiORF®
Lenti ORF clone of Human thyroid peroxidase (TPO), transcript variant 1, mGFP tagged
"NM_000547" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | Thyroid Peroxidase |
Synonyms | MSA; TDH2A; TPX |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210659).
|
Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000547 |
ORF Size | 2799 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000547.3 |
RefSeq Size | 3152 bp |
RefSeq ORF | 2802 bp |
Locus ID | 7173 |
UniProt ID | P07202 |
Cytogenetics | 2p25.3 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Metabolic pathways, Tyrosine metabolism |
MW | 102.9 kDa |
Gene Summary | This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210659 | TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 1 |
USD 854.00 |
|
RC210659L1 | Lenti ORF clone of Human thyroid peroxidase (TPO), transcript variant 1, Myc-DDK-tagged |
USD 1,154.00 |
|
RC210659L2 | Lenti ORF clone of Human thyroid peroxidase (TPO), transcript variant 1, mGFP tagged |
USD 1,154.00 |
|
RC210659L3 | Lenti ORF clone of Human thyroid peroxidase (TPO), transcript variant 1, Myc-DDK-tagged |
USD 1,154.00 |
|
RG210659 | TPO (tGFP-tagged) - Human thyroid peroxidase (TPO), transcript variant 1 |
USD 1,054.00 |
|
SC300094 | TPO (untagged)-Human thyroid peroxidase (TPO), transcript variant 1 |
USD 855.00 |
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