FAM40A (STRIP1) (NM_033088) Human Tagged ORF Clone

CAT#: RC210537

STRIP1 (Myc-DDK-tagged)-Human family with sequence similarity 40, member A (FAM40A)

Product Images

Circular map for RC210537

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FAM40A (Cat# RC210537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM40A(Cat# TA502310). Positive lysates LY409738 (100ug) and LC409738 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409738) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210537 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STRIP1 protein (Cat# TP310537). The protein was produced from HEK293T cells transfected with STRIP1 cDNA clone (Cat# RC210537) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM40A
• Synonyms: FAM40A; FAR11A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210537 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGCAGTCGGCGGTCCGGGCCCACTGATCGTGAACAACAAACAGCCCCAGCCCCCGCCACCTC
  CGCCGCCGGCAGCCGCACAGCCACCACCCGGGGCACCGCGGGCCGCCGCGGGCCTCCTGCCTGGGGGCAA
  AGCCCGCGAGTTCAACCGCAACCAGCGCAAAGACTCAGAGGGCTATTCGGAGTCACCAGACCTGGAGTTT
  GAGTATGCTGACACAGACAAGTGGGCTGCAGAGCTCTCGGAGCTTTACAGCTACACGGAAGGGCCAGAAT
  TCCTGATGAATCGAAAATGCTTTGAGGAGGACTTCCGGATCCATGTGACAGACAAGAAGTGGACTGAGCT
  GGATACCAACCAGCACCGGACCCATGCCATGAGGCTCCTGGATGGCTTGGAAGTCACTGCCAGGGAGAAG
  AGACTCAAGGTGGCTCGAGCAATTCTCTATGTTGCTCAAGGCACGTTTGGGGAGTGCAGCTCGGAGGCAG
  AGGTGCAGTCCTGGATGCGCTACAACATCTTTCTCCTCCTGGAGGTGGGCACGTTCAATGCTTTGGTGGA
  GCTTCTGAACATGGAAATAGACAACAGTGCCGCCTGCAGCAGTGCTGTGAGGAAGCCTGCCATCTCCCTG
  GCTGACAGCACAGACCTCAGGGTCCTGCTCAACATCATGTACCTGATAGTGGAGACCGTTCATCAGGAGT
  GTGAGGGTGACAAGGCTGAGTGGAGGACCATGCGGCAGACCTTCAGAGCCGAGCTGGGCTCCCCGCTGTA
  CAACAATGAGCCATTTGCCATCATGCTGTTTGGGATGGTGACCAAATTTTGCAGTGGTCACGCCCCTCAC
  TTTCCCATGAAGAAAGTTCTCTTGCTGCTCTGGAAGACAGTATTGTGCACGCTAGGCGGCTTTGAGGAGC
  TGCAGAGCATGAAGGCTGAGAAGCGCAGCATCCTGGGCCTCCCCCCGCTTCCTGAGGACAGCATCAAAGT
  GATTCGCAACATGAGAGCAGCCTCTCCACCAGCATCTGCTTCAGACTTGATTGAGCAGCAGCAGAAACGG
  GGCCGCCGAGAGCACAAGGCTCTGATAAAGCAGGACAACCTAGATGCCTTCAACGAGCGGGATCCCTACA
  AGGCTGATGACTCTCGAGAAGAGGAAGAGGAGAATGATGATGACAACAGTCTGGAGGGGGAGACGTTTCC
  CCTGGAACGGGATGAAGTGATGCCTCCCCCGCTACAGCACCCACAGACTGACAGGCTGACTTGCCCCAAA
  GGGCTCCCGTGGGCTCCCAAGGTCAGAGAGAAAGACATTGAGATGTTCCTTGAGTCCAGCCGCAGCAAAT
  TTATAGGTTACACTCTAGGCAGTGACACGAACACAGTGGTGGGGCTGCCCAGGCCAATCCACGAAAGCAT
  CAAGACTCTGAAACAGCACAAGTACACGTCGATTGCAGAGGTCCAGGCACAGATGGAGGAGGAATACCTC
  CGCTCCCCTCTCTCAGGGGGAGAAGAAGAAGTTGAGCAAGTCCCTGCAGAAACCCTCTACCAAGGCTTGC
  TCCCCAGCCTGCCTCAGTATATGATTGCCCTCCTGAAGATCCTGTTGGCTGCAGCACCCACCTCAAAAGC
  CAAAACAGACTCAATCAACATCCTAGCGGACGTCTTGCCTGAGGAGATGCCCACCACAGTGTTGCAGAGC
  ATGAAGCTGGGGGTGGATGTAAACCGCCACAAAGAGGTCATTGTTAAGGCCATTTCTGCTGTCCTGCTGC
  TGCTGCTCAAGCACTTTAAGTTGAACCATGTCTACCAGTTTGAATACATGGCCCAGCACCTGGTGTTTGC
  CAACTGCATTCCTTTGATCCTAAAGTTCTTCAATCAAAACATCATGTCCTACATCACTGCCAAGAACAGC
  ATTTCTGTCCTGGATTACCCTCACTGCGTGGTGCATGAGCTGCCAGAGCTGACGGCGGAGAGTTTGGAAG
  CAGGTGACAGTAACCAATTTTGCTGGAGGAACCTCTTTTCTTGTATCAATCTGCTTCGGATCTTGAACAA
  GCTGACAAAGTGGAAGCATTCAAGGACAATGATGCTGGTGGTGTTCAAGTCAGCCCCCATCTTGAAGCGG
  GCCCTAAAGGTGAAACAAGCCATGATGCAGCTCTATGTGCTGAAGCTGCTCAAGGTACAGACCAAATACT
  TGGGGCGGCAGTGGCGAAAGAGCAACATGAAGACCATGTCTGCCATCTACCAGAAGGTGCGGCATCGGCT
  GAACGACGACTGGGCATACGGCAATGATCTTGATGCCCGGCCTTGGGACTTCCAGGCAGAGGAGTGTGCC
  CTTCGTGCCAACATTGAACGCTTCAACGCCCGGCGCTATGACCGGGCCCACAGCAACCCTGACTTCCTGC
  CAGTGGACAACTGCCTGCAGAGTGTCCTGGGCCAACGGGTGGACCTCCCTGAGGACTTTCAGATGAACTA
  TGACCTCTGGTTAGAAAGGGAGGTCTTCTCCAAGCCCATTTCCTGGGAAGAGCTGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210537 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPAVGGPGPLIVNNKQPQPPPPPPPAAAQPPPGAPRAAAGLLPGGKAREFNRNQRKDSEGYSESPDLEF
  EYADTDKWAAELSELYSYTEGPEFLMNRKCFEEDFRIHVTDKKWTELDTNQHRTHAMRLLDGLEVTAREK
  RLKVARAILYVAQGTFGECSSEAEVQSWMRYNIFLLLEVGTFNALVELLNMEIDNSAACSSAVRKPAISL
  ADSTDLRVLLNIMYLIVETVHQECEGDKAEWRTMRQTFRAELGSPLYNNEPFAIMLFGMVTKFCSGHAPH
  FPMKKVLLLLWKTVLCTLGGFEELQSMKAEKRSILGLPPLPEDSIKVIRNMRAASPPASASDLIEQQQKR
  GRREHKALIKQDNLDAFNERDPYKADDSREEEEENDDDNSLEGETFPLERDEVMPPPLQHPQTDRLTCPK
  GLPWAPKVREKDIEMFLESSRSKFIGYTLGSDTNTVVGLPRPIHESIKTLKQHKYTSIAEVQAQMEEEYL
  RSPLSGGEEEVEQVPAETLYQGLLPSLPQYMIALLKILLAAAPTSKAKTDSINILADVLPEEMPTTVLQS
  MKLGVDVNRHKEVIVKAISAVLLLLLKHFKLNHVYQFEYMAQHLVFANCIPLILKFFNQNIMSYITAKNS
  ISVLDYPHCVVHELPELTAESLEAGDSNQFCWRNLFSCINLLRILNKLTKWKHSRTMMLVVFKSAPILKR
  ALKVKQAMMQLYVLKLLKVQTKYLGRQWRKSNMKTMSAIYQKVRHRLNDDWAYGNDLDARPWDFQAEECA
  LRANIERFNARRYDRAHSNPDFLPVDNCLQSVLGQRVDLPEDFQMNYDLWLEREVFSKPISWEELLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033088
• ORF Size: 2511 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033088.4
• RefSeq Size: 3291 bp
• RefSeq ORF: 2514 bp
• Locus ID: 85369
• UniProt ID: Q5VSL9
• Cytogenetics: 1p13.3
• MW: 95.6 kDa
• Gene Summary: This gene encodes a member of the striatin-interacting phosphatase and kinase complex, which is involved in localization of the Golgi body. The encoded protein participates in cytosketelal organization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]