NEDD8 (NM_006156) Human Tagged Lenti ORF Clone
CAT#: RC210479L2
- LentiORF®
Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), mGFP tagged
"NM_006156" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | NEDD8 |
Synonyms | NEDD-8 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210479).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006156 |
ORF Size | 243 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006156.1 |
RefSeq Size | 625 bp |
RefSeq ORF | 246 bp |
Locus ID | 4738 |
UniProt ID | Q15843 |
Cytogenetics | 14q12 |
Protein Families | Druggable Genome |
MW | 9.1 kDa |
Gene Summary | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210479 | NEDD8 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) |
USD 150.00 |
|
RC210479L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), Myc-DDK-tagged |
USD 450.00 |
|
RC210479L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), Myc-DDK-tagged |
USD 450.00 |
|
RC210479L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), mGFP tagged |
USD 450.00 |
|
RG210479 | NEDD8 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) |
USD 350.00 |
|
SC110960 | NEDD8 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) |
USD 150.00 |
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