hnRNP L (HNRNPL) (NM_001005335) Human Tagged ORF Clone
CAT#: RC209067
- TrueORF®
HNRNPL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001005335" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | hnRNP L |
Synonyms | hnRNP-L; HNRPL; P/OKcl.14 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209067 representing NM_001005335
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTAAAAAGAGACAAGCACTGGTGGAGTTTGAAGATGTGTTGGGGGCTTGCAACGCAGTGAACTACG CAGCCGACAACCAAATATACATTGCTGGTCACCCAGCTTTTGTCAACTACTCTACCAGCCAGAAGATCTC CCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGCTTCTCTTTACCATCCTGAACCCCATT TATTCGATCACCACGGATGTTCTTTACACTATCTGTAATCCTTGTGGCCCTGTCCAGAGAATTGTCATTT TCAGGAAGAATGGAGTTCAGGCGATGGTGGAATTTGACTCAGTTCAAAGTGCCCAGCGGGCCAAGGCCTC TCTCAATGGGGCTGATATCTATTCTGGCTGTTGCACTCTGAAGATCGAATACGCAAAGCCTACACGCTTG AATGTGTTCAAGAATGATCAGGATACTTGGGACTACACAAACCCCAATCTCAGTGGACAAGGTGACCCTG GCAGCAACCCCAACAAACGCCAGAGGCAGCCCCCTCTCCTGGGAGATCACCCCGCAGAATATGGAGGGCC CCACGGTGGGTACCACAGCCATTACCATGATGAGGGCTACGGGCCCCCCCCACCTCACTACGAAGGGAGA AGGATGGGTCCACCAGTGGGGGGTCACCGTCGGGGCCCAAGTCGCTACGGCCCCCAGTATGGGCACCCCC CACCCCCTCCCCCACCACCCGAGTATGGCCCTCACGCCGACAGCCCTGTGCTCATGGTCTATGGCTTGGA TCAATCTAAGATGAACTGTGACCGAGTCTTCAATGTCTTCTGCTTATATGGCAATGTGGAGAAGGTGAAA TTCATGAAAAGCAAGCCGGGGGCCGCCATGGTGGAGATGGCTGATGGCTACGCTGTAGACCGGGCCATTA CCCACCTCAACAACAACTTCATGTTTGGGCAGAAGCTGAATGTCTGTGTCTCCAAGCAGCCAGCCATCAT GCCTGGTCAGTCATACGGGTTGGAAGACGGGTCTTGCAGTTACAAAGACTTCAGTGAATCCCGGAACAAT CGGTTCTCCACCCCAGAGCAGGCAGCCAAGAACCGCATCCAGCACCCCAGCAACGTGCTGCACTTCTTCA ACGCCCCGCTGGAGGTGACCGAGGAGAACTTCTTTGAGATCTGCGATGAGCTGGGAGTGAAGCGGCCATC TTCTGTGAAAGTATTCTCAGGCAAAAGTGAGCGCAGCTCCTCTGGACTGCTGGAGTGGGAATCCAAGAGC GATGCCCTGGAGACTCTGGGCTTCCTGAACCATTACCAGATGAAAAACCCAAATGGTCCATACCCTTACA CTCTGAAGTTGTGTTTCTCCACTGCTCAGCACGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209067 representing NM_001005335
Red=Cloning site Green=Tags(s) MPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPI YSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRL NVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGR RMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVK FMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNN RFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKS DALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001005335 |
ORF Size | 1368 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005335.2 |
RefSeq Size | 1895 bp |
RefSeq ORF | 1371 bp |
Locus ID | 3191 |
UniProt ID | P14866 |
Cytogenetics | 19q13.2 |
MW | 50.6 kDa |
Gene Summary | Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209067L3 | Lenti-ORF clone of HNRNPL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2 |
USD 757.00 |
|
RC209067L4 | Lenti-ORF clone of HNRNPL (mGFP-tagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2 |
USD 757.00 |
|
RG209067 | HNRNPL (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2 |
USD 657.00 |
|
SC321967 | HNRNPL (untagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2 |
USD 457.00 |
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