Peregrin (BRPF1) (NM_001003694) Human Tagged ORF Clone

CAT#: RC208775

BRPF1 (Myc-DDK-tagged)-Human bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 1

Product Images

Circular map for RC208775

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Peregrin
• Synonyms: BR140; IDDDFP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208775 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCATACGAGTGCC
  CGGTGGAGACCTGCCGAAAGGTCTACAAGAGTTACAGTGGTATTGAGTACCACCTGTACCACTATGACCA
  CGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAGCACAAGAAAAAGGGGCGCCAGTCACGCCCA
  GCCAACAAGCAGTCACCCAGCCCCTCAGAGGTCTCACAGTCACCAGGCCGTGAGGTGATGAGCTATGCAC
  AGGCCCAGCGCATGGTGGAGGTGGACTTGCATGGCCGCGTCCACCGCATCAGCATCTTTGACAACCTGGA
  TGTGGTGTCAGAGGATGAGGAAGCCCCCGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAGAACACTGAG
  ACACCAGCTGCTACTCCCAAGTCAGGCAAACATAAGAACAAGGAGAAGCGCAAGGACTCCAACCATCACC
  ACCACCACAATGTTTCTGCGAGCACCACTCCCAAGCTGCCAGAGGTGGTCTATCGGGAGCTGGAACAGGA
  CACCCCTGATGCCCCACCCCGGCCAACTTCCTATTACCGGTACATCGAGAAGTCTGCAGAGGAGCTGGAC
  GAGGAAGTAGAGTATGACATGGACGAGGAGGACTACATCTGGCTGGATATCATGAATGAGCGTCGGAAGA
  CAGAGGGTGTAAGTCCCATCCCGCAGGAGATCTTTGAGTACCTAATGGACCGACTGGAGAAGGAGTCGTA
  CTTTGAGAGTCATAATAAAGGCGACCCTAATGCGCTAGTGGACGAGGATGCTGTTTGCTGTATCTGCAAT
  GATGGTGAGTGCCAGAACAGCAATGTCATCCTCTTCTGTGACATGTGCAACCTGGCCGTGCACCAGGAGT
  GCTACGGTGTCCCCTATATCCCTGAGGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCCTCTCGTGC
  TGTGGATTGTGCCCTGTGCCCCAACAAGGGCGGTGCCTTCAAGCAGACAGATGACGGGCGCTGGGCCCAT
  GTGGTGTGTGCCTTGTGGATCCCTGAGGTCTGCTTCGCCAACACGGTCTTCCTAGAGCCTATTGACAGCA
  TTGAGCACATCCCACCAGCTCGCTGGAAGCTCACCTGCTACATTTGCAAACAACGGGGCTCAGGGGCCTG
  CATCCAGTGCCACAAGGCCAACTGTTACACAGCTTTCCATGTGACATGCGCCCAGCAGGCTGGCCTTTAC
  ATGAAGATGGAGCCTGTGCGGGAGACAGGCGCCAACGGCACCTCTTTCAGTGTCCGCAAGACAGCCTACT
  GCGACATCCACACGCCTCCAGGTTCAGCACGCCGACTGCCTGCCCTGTCCCACAGCGAGGGTGAGGAGGA
  TGAAGATGAGGAGGAGGATGAGGGTAAGGGCTGGAGCTCAGAGAAAGTCAAGAAGGCCAAGGCCAAGTCC
  CGGATCAAAATGAAGAAGGCACGGAAGATCCTGGCAGAGAAGCGGGCAGCAGCACCTGTGGTGTCAGTGC
  CCTGCATCCCACCACACAGGCTTAGTAAAATCACCAACCGCCTGACCATCCAAAGGAAGAGCCAGTTCAT
  GCAGAGGCTGCACAGCTACTGGACACTGAAGCGGCAGTCACGGAATGGGGTCCCATTGCTACGTCGCCTG
  CAGACACACCTGCAATCTCAGAGGAACTGTGACCAAGTTGGGAGAGATTCTGAGGATAAGAACTGGGCCC
  TTAAAGAACAGCTCAAGTCCTGGCAGCGGCTCCGGCATGACTTGGAGCGAGCTCGGCTGCTCGTGGAATT
  GATCCGCAAGCGGGAAAAACTCAAAAGGGAGACGATCAAGGTTCAGCAGATTGCCATGGAGATGCAGCTG
  ACTCCTTTCCTCATCCTCCTTCGCAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCA
  GCGAGCCGGTCCCTCTGTCTGAGGTAACCGAATTGGACGAAGTACCTGACTACCTAGACCACATCAAAAA
  GCCCATGGACTTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACCTGAATTTTGATGATTTTGAG
  GAGGACTTCAACCTCATCGTCAGCAACTGCCTCAAGTATAACGCCAAGGACACCATCTTCTACCGGGCAG
  CAGTGCGGCTTCGTGAGCAGGGTGGTGCTGTGCTCCGCCAGGCCCGGCGCCAGGCAGAAAAAATGGGCAT
  TGACTTTGAGACGGGCATGCATATCCCCCACAGCCTGGCTGGAGATGAGGCCACACACCACACTGAAGAT
  GCAGCCGAGGAAGAGCGGCTGGTCTTGCTGGAGAACCAGAAGCACCTGCCAGTGGAAGAACAGCTAAAGC
  TGCTTCTGGAGCGGCTGGACGAAGTGAATGCCAGCAAGCAGAGTGTGGGCCGCTCACGGCGTGCAAAGAT
  GATCAAGAAAGAGATGACGGCACTGCGGCGGAAGCTTGCCCATCAGCGAGAGACGGGACGTGATGGCCCT
  GAGCGGCATGGCCCCTCGAGCCGGGGTAGTCTGACACCCCACCCGGCAGCCTGTGACAAGGATGGGCAGA
  CAGATAGTGCGGCAGAGGAGAGCAGCAGCCAGGAGACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAAC
  CCCCGCACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCG
  CCCAAGAGGCCGGGCCGGCCCCCCAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTG
  TGGGGCCCCCCCAGCTCCCCATCATGAGTTCCCTGCGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAG
  TTCGAGCTCAGACAGCGACAGTGATAAGTCCACAGAAGACCCCCCAATGGACTTACCAGCCAATGGCTTC
  AGCGGTGGAAACCAACCAGTGAAGAAGAGTTTCTTGGTATACCGTAATGACTGCAGCCTTCCCCGGAGCA
  GCTCAGACTCTGAGTCCAGCAGCAGTAGCAGTAGCAGCGCTGCTTCAGACCGGACCAGCACAACGCCCTC
  AAAACAAGGCCGGGGCAAACCCTCCTTCTCTCGGGGCACTTTCCCAGAGGACAGCAGTGAGGATACCTCA
  GGCACTGAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCATGGTAAGGAAGAGTC
  TGGGCCGGGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGCTGGATGCTCTGGACCTCGTGTGGGC
  CAAATGCCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTC
  CACCATGGGGTTCCCATCCCTGTGCCCCCACTGGAGGTGCTGAAACTTGGGGAGCAGATGACCCAGGAAG
  CCCGAGAGCATCTCTACCTCGTCCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCAGGACCAA
  GCTGGTTCCTCTGGGTGTGAACCAGGACCTAGACAAGGAGAAGATGCTGGAGGGCCGCAAGTCCAACATC
  CGCAAGTCAGTACAGATCGCCTACCACAGGGCTCTGCAGCACCGCAGCAAGGTGCAAGGCGAGCAGAGCA
  GTGAGACCAGCGATAGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208775 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQSRP
  ANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTE
  TPAATPKSGKHKNKEKRKDSNHHHHHNVSASTTPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELD
  EEVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICN
  DGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAH
  VVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLY
  MKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEDEDEEEDEGKGWSSEKVKKAKAKS
  RIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRL
  QTHLQSQRNCDQVGRDSEDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQIAMEMQL
  TPFLILLRKTLEQLQEKDTGNIFSEPVPLSEVTELDEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFE
  EDFNLIVSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHSLAGDEATHHTED
  AAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGP
  ERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGP
  PKRPGRPPKNRESQMTPSHGGSPVGPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGF
  SGGNQPVKKSFLVYRNDCSLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTS
  GTENEAYSVGTGRGVGHSMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYPALIIDPKMPREGMF
  HHGVPIPVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGVNQDLDKEKMLEGRKSNI
  RKSVQIAYHRALQHRSKVQGEQSSETSDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003694
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003694.2
• RefSeq Size: 4728 bp
• RefSeq ORF: 3663 bp
• Locus ID: 7862
• UniProt ID: P55201
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 138.2 kDa
• Gene Summary: This gene encodes a bromodomain, PHD finger and chromo/Tudor-related Pro-Trp-Trp-Pro (PWWP) domain containing protein. The encoded protein is a component of the MOZ/MORF histone acetyltransferase complexes which function as a transcriptional regulators. This protein binds to the catalytic MYST domains of the MOZ and MORF proteins and may play a role in stimulating acetyltransferase and transcriptional activity of the complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]