WDR54 (NM_032118) Human Tagged ORF Clone
CAT#: RC208682
WDR54 (Myc-DDK-tagged)-Human WD repeat domain 54 (WDR54)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_032118" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | WDR54 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208682 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGCTGGGAGCGCTCCATTCCCCTGCGAGGCTCGGCCGCCGCCCTGTGCAACAACCTCAGTGTGC TGCAGCTGCCGGCTCGCAACCTCACGTATTTTGGCGTGGTTCATGGACCAAGCGCCCAGCTTCTCAGCGC TGCTCCTGAGGGTGTGCCCTTGGCCCAGCGCCAGCTCCACGCTAAGGAGGGTGCTGGAGTGAGTCCCCCA CTTATCACTCAGGTCCACTGGTGTGTCCTCCCCTTCCGAGTGCTGCTGGTACTCACCTCACATCGAGGAA TACAGATGTACGAGTCCAATGGCTACACCATGGTCTACTGGCATGCACTGGACTCTGGAGATGCCTCCCC AGTACAGGCTGTGTTTGCCCGGGGAATTGCTGCCAGTGGCCACTTCATCTGTGTGGGAACGTGGTCAGGC CGGGTGCTGGTGTTTGACATCCCAGCAAAGGGTCCCAACATTGTACTGAGCGAGGAGCTGGCTGGGCACC AGATGCCAATCACAGACATTGCCACCGAGCCTGCCCAGGGACAGGATTGTGTGGCTGACATGGTGACGGC AGATGACTCAGGCTTGCTGTGTGTCTGGCGGTCAGGGCCAGAATTCACATTATTGACCCGCATTCCAGGA TTTGGAGTTCCGTGCCCCTCTGTGCAGCTGTGGCAGGGGATCATAGCAGCAGGCTATGGGAACGGACAAG TGCATCTATATGAGGCCACTACAGGAAATCTACATGTCCAGATCAATGCCCATGCCCGGGCCATCTGCGC CCTGGACCTGGCTTCTGAGGTGGGCAAGCTACTCTCTGCAGGTGAGGACACCTTTGTGCATATCTGGAAG CTGAGCAGAAACCCAGAGAGTGGCTACATTGAGGTGGAACACTGTCATGGTGAGTGTGTCGCCGACACCC AGCTGTGTGGTGCTCGATTTTGTGATTCCTCAGGCAACTCCTTTGCTGTGACTGGCTATGACCTTGCGGA GATCCGGAGATTCAGCAGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208682 protein sequence
Red=Cloning site Green=Tags(s) MFRWERSIPLRGSAAALCNNLSVLQLPARNLTYFGVVHGPSAQLLSAAPEGVPLAQRQLHAKEGAGVSPP LITQVHWCVLPFRVLLVLTSHRGIQMYESNGYTMVYWHALDSGDASPVQAVFARGIAASGHFICVGTWSG RVLVFDIPAKGPNIVLSEELAGHQMPITDIATEPAQGQDCVADMVTADDSGLLCVWRSGPEFTLLTRIPG FGVPCPSVQLWQGIIAAGYGNGQVHLYEATTGNLHVQINAHARAICALDLASEVGKLLSAGEDTFVHIWK LSRNPESGYIEVEHCHGECVADTQLCGARFCDSSGNSFAVTGYDLAEIRRFSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032118 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032118.4 |
RefSeq Size | 1223 bp |
RefSeq ORF | 1005 bp |
Locus ID | 84058 |
UniProt ID | Q9H977 |
Cytogenetics | 2p13.1 |
Domains | WD40 |
MW | 35.9 kDa |
Gene Summary | When cross-linked to form dimers and trimers, it has a regulatory effect on ERK signaling pathway activity in response to EGF stimulation. Colocalizes with the EGF receptor in WDR54-specific vesicle where it sustains the internalization and controls the degradation of the EGF receptor after EGF stimulation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208682L3 | Lenti ORF clone of Human WD repeat domain 54 (WDR54), Myc-DDK-tagged |
USD 757.00 |
|
RC208682L4 | Lenti ORF clone of Human WD repeat domain 54 (WDR54), mGFP tagged |
USD 757.00 |
|
RG208682 | WDR54 (tGFP-tagged) - Human WD repeat domain 54 (WDR54) |
USD 657.00 |
|
SC106524 | WDR54 (untagged)-Human WD repeat domain 54 (WDR54) |
USD 457.00 |
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