MAGEE1 (NM_020932) Human Tagged ORF Clone

CAT#: RC208544

MAGEE1 (Myc-DDK-tagged)-Human melanoma antigen family E, 1 (MAGEE1)

Product Images

Circular map for RC208544

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGEE1
• Synonyms: DAMAGE; HCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208544 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGGTAAGCCAGAATTCGCGCCGCCGCCGCCGCCGCGTTGCAAAGGCTACTGCGCACAACAGCA
  GCTGGGGCGAAATGCAGGCCCCTAATGCCCCCGGTCTCCCCGCTGATGTGCCAGGCTCAGACGTCCCCCA
  GGGTCCCAGCGATTCCCAGATCCTCCAGGGCCTCTGCGCCTCTGAGGGCCCAAGCACCTCCGTTCTGCCC
  ACCTCCGCTGAGGGCCCCAGCACCTTTGTGCCGCCCACCATCTCTGAGGCCTCAAGCGCCTCCGGGCAGC
  CCACCATCTCTGAGGGACCTGGCACCTCCGTGCTGCCCACCCCCAGTGAGGGCCTAAGCACCTCCGGGCC
  TCCCACCATCTCTAAGGGGCTGTGCACCTCTGTGACGCTTGCCGCCTCTGAGGGCCGGAACACCTCCAGG
  CCGCCCACTTCCTCTGAGGAACCTAGCACCTCCGTGCCGCCCACCGCCTCTGAGGTACCGAGCACCTCCC
  TGCCGCCCACCCCTGGTGAGGGAACGAGCACCTCCGTGCCGCCCACAGCCTATGAGGGACCAAGCACCTC
  CGTGGTGCCCACCCCTGATGAGGGACCAAGCACCTCCGTGCTGCCTACACCTGGTGAGGGACCAGGCACC
  TCCGTGCCGCTCGCCGCCACTGAGGGCCTGAGCACCTCCGTGCAGGCCACTCCTGATGAGGGACCGAGCA
  CCTCCGTGCCGCCCACCGCCACTGAGGGCCTAAGCACCCCCGTGCCACCCACCCGTGATGAGGGACCGAG
  CACCTCCGTGCCGGCCACTCCTGGTGAGGGACCGAGCACCTCCGTGCTGCCCGCCGCCTCTGACGGACAA
  AGCATCTCCTTGGTGCCCACCCGCGGTAAGGGATCAAGCACCTCCGTGCCCCCCACCGCCACCGAGGGCC
  TGAGCACCTCCGTGCAGCCCACTGCTGGTGAGGGATCGAGCACCTCCGTGCCGCCCACCCCTGGTGGGGG
  ACTGAGCACCTCCGTGCCGCCCACCGCCACTGAGGACTTGAGCACCTCCGTGCCGCCCACTCCCGGTGAG
  GGACCAAGCACTTCCGTACTGCCAATCCCCGGTGAGGGACTGAGCACCTCTGTGCCGCCCACCGCCTCTG
  ATGGATCGGACACCTCCGTGCCGCCCACTCCTGGTGAGGGCGCAAGCACCTTAGTGCAGCCCACCGCCCC
  TGACGGACCGGGAAGCTCCGTGCTGCCTAACCCTGGTGAGGGCCCGAGCACATTGTTTAGCTCTAGTGCT
  TCTGTGGACCGGAACCCCTCCAAGTGTTCCCTTGTTTTGCCAAGCCCTAGGGTAACCAAGGCCTCCGTGG
  ACTCAGATTCTGAGGGTCCTAAGGGTGCAGAAGGCCCTATAGAATTCGAGGTCCTGAGAGACTGTGAGAG
  CCCCAACTCCATTAGTATTATGGGCCTCAATACTTCCCGGGTTGCAATTACCCTGAAGCCCCAAGACCCT
  ATGGAACAGAACGTAGCTGAGCTGTTGCAGTTCCTGCTGGTGAAGGATCAGAGCAAGTACCCTATCCGGG
  AGTCTGAAATGCGGGAATATATTGTTAAAGAATATCGCAACCAGTTTCCTGAGATACTCAGGCGAGCAGC
  AGCCCACCTGGAGTGCATTTTTAGGTTTGAATTGAGAGAACTTGACCCTGAGGCACACACCTACATTCTG
  TTAAACAAACTGGGACCTGTGCCCTTTGAAGGGTTAGAAGAGAGCCCAAATGGGCCAAAGATGGGCCTCC
  TGATGATGATTCTAGGCCAAATATTCCTGAATGGCAACCAAGCCAAGGAGGCTGAGATTTGGGAAATGCT
  CTGGAGGATGGGGGTGCAGCGGGAAAGGAGGCTTTCCATTTTTGGGAACCCAAAGAGACTTCTGTCTGTG
  GAGTTTGTATGGCAGCGTTACTTAGACTACAGGCCAGTAACTGACTGTAAACCAGTGGAGTATGAGTTTT
  TCTGGGGCCCAAGATCCCACCTAGAAACCACCAAGATGAAAATTCTGAAGTTCATGGCGAAAATATATAA
  CAAAGATCCTATGGATTGGCCAGAGAAATACAACGAAGCTCTGGAAGAAGATGCTGCCAGAGCCTTTGCT
  GAGGGTTGGCAGGCTCTCCCTCACTTTAGGAGGCCCTTTTTTGAGGAAGCTGCTGCAGAGGTACCATCCC
  CTGATTCAGAGGTTTCCAGCTATTCCTCAAAATATGCCCCACATTCATGGCCTGAGTCAAGATTGGAGAG
  CAAGGCAAGGAAGCTGGTGCAGTTATTTCTGCTTATGGATTCAACTAAGCTGCCTATACCAAAGAAAGGA
  ATTCTGTACTACATTGGCCGAGAGTGCAGCAAAGTGTTCCCTGACCTCCTGAATCGTGCTGCCCGCACCC
  TGAACCATGTCTATGGGACAGAACTAGTGGTACTTGATCCCAGGAATCACTCCTATACTCTGTACAACCG
  AAGGGAGATGGAAGAAACTGAGGAGATCGTAGACAGTCCAAACAGGCCTGGCAACAACTTTTTGATGCAG
  GTCCTAAGCTTCATCTTTATTATGGGCAACCATGCCAGGGAGTCTGCAGTCTGGGCCTTTCTGCGGGGCT
  TAGGGGTTCAAGCTGGGAGAAAGCATGTGATTACCTGCAGATACTTGAGTCAGCGCTATATAGACAGTTT
  ACGGGTTCCTGACAGTGATCCAGTGCAATATGAGTTTGTATGGGGTCCTAGAGCCCGTTTGGAAACCTCT
  AAGATGAAAGCCTTGCGATATGTGGCCAGAATCCACAGAAAGGAACCACAGGACTGGCCACAGCAGTACA
  GGGAGGCAATGGAAGATGAGGCCAATAGAGCTGATGTTGGGCACAGGCAAATCTTTGTTCACAACTTCAG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208544 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLVSQNSRRRRRRVAKATAHNSSWGEMQAPNAPGLPADVPGSDVPQGPSDSQILQGLCASEGPSTSVLP
  TSAEGPSTFVPPTISEASSASGQPTISEGPGTSVLPTPSEGLSTSGPPTISKGLCTSVTLAASEGRNTSR
  PPTSSEEPSTSVPPTASEVPSTSLPPTPGEGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEGPGT
  SVPLAATEGLSTSVQATPDEGPSTSVPPTATEGLSTPVPPTRDEGPSTSVPATPGEGPSTSVLPAASDGQ
  SISLVPTRGKGSSTSVPPTATEGLSTSVQPTAGEGSSTSVPPTPGGGLSTSVPPTATEDLSTSVPPTPGE
  GPSTSVLPIPGEGLSTSVPPTASDGSDTSVPPTPGEGASTLVQPTAPDGPGSSVLPNPGEGPSTLFSSSA
  SVDRNPSKCSLVLPSPRVTKASVDSDSEGPKGAEGPIEFEVLRDCESPNSISIMGLNTSRVAITLKPQDP
  MEQNVAELLQFLLVKDQSKYPIRESEMREYIVKEYRNQFPEILRRAAAHLECIFRFELRELDPEAHTYIL
  LNKLGPVPFEGLEESPNGPKMGLLMMILGQIFLNGNQAKEAEIWEMLWRMGVQRERRLSIFGNPKRLLSV
  EFVWQRYLDYRPVTDCKPVEYEFFWGPRSHLETTKMKILKFMAKIYNKDPMDWPEKYNEALEEDAARAFA
  EGWQALPHFRRPFFEEAAAEVPSPDSEVSSYSSKYAPHSWPESRLESKARKLVQLFLLMDSTKLPIPKKG
  ILYYIGRECSKVFPDLLNRAARTLNHVYGTELVVLDPRNHSYTLYNRREMEETEEIVDSPNRPGNNFLMQ
  VLSFIFIMGNHARESAVWAFLRGLGVQAGRKHVITCRYLSQRYIDSLRVPDSDPVQYEFVWGPRARLETS
  KMKALRYVARIHRKEPQDWPQQYREAMEDEANRADVGHRQIFVHNFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020932
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020932.3
• RefSeq Size: 3714 bp
• RefSeq ORF: 2874 bp
• Locus ID: 57692
• UniProt ID: Q9HCI5
• Cytogenetics: Xq13.3
• MW: 103.2 kDa
• Gene Summary: This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein, which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus, 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT, and two MAGE domains in the C-terminus. It may play a signaling role in brain, muscle, and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to cognitive disability. [provided by RefSeq, Mar 2010]