ANGPTL5 (NM_178127) Human Tagged ORF Clone

CAT#: RC208478

ANGPTL5 (Myc-DDK-tagged)-Human angiopoietin-like 5 (ANGPTL5)

Product Images

Circular map for RC208478

• 

  Western blot validation of overexpression lysate (Cat# LY403602) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208478 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANGPTL5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208478 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCTCCATCCCAAGCCTCACTCTTATTCTTAAATGTATGTATTTTTATTTGTGGAGAAGCTGTAC
  AAGGTAACTGTGTACATCATTCTACGGACTCTTCAGTAGTTAACATTGTAGAAGATGGATCTAATGCAAA
  AGATGAAAGTAAAAGTAATGATACTGTTTGTAAGGAAGACTGTGAGGAATCATGTGATGTTAAAACTAAA
  ATTACACGAGAAGAAAAACATTTCATGTGTAGAAATTTGCAAAATTCTATTGTTTCCTACACAAGAAGTA
  CCAAAAAACTACTAAGGAATATGATGGATGAGCAACAAGCTTCCTTGGATTATTTATCTAATCAGGTTAA
  CGAGCTCATGAATAGAGTTCTCCTTTTGACTACAGAAGTTTTTAGAAAACAGCTGGATCCTTTTCCTCAC
  AGACCTGTTCAGTCACATGGTTTAGATTGCACTGATATTAAGGATACCATTGGCTCTGTCACCAAAACAC
  CGAGTGGTTTATACATAATTCACCCAGAAGGATCTAGCTACCCATTTGAGGTAATGTGTGACATGGATTA
  CAGAGGAGGTGGATGGACCGTGATACAGAAAAGAATTGATGGGATAATTGATTTCCAGAGGTTGTGGTGT
  GATTATCTGGATGGATTTGGAGATCTTCTAGGAGAATTTTGGCTAGGACTGAAAAAGATTTTTTATATAG
  TAAATCAGAAAAATACCAGTTTTATGCTGTATGTGGCTTTGGAATCTGAAGATGACACTCTTGCTTATGC
  ATCATATGATAATTTTTGGCTAGAGGATGAAACGAGATTTTTTAAAATGCACTTAGGACGGTATTCAGGA
  AATGCTGGTGATGCATTCCGGGGTCTCAAAAAAGAAGATAATCAAAATGCAATGCCTTTTAGCACATCAG
  ATGTTGATAATGATGGGTGTCGCCCTGCATGCCTGGTCAATGGTCAGTCTGTGAAGAGCTGCAGTCACCT
  CCATAACAAGACCGGCTGGTGGTTTAACGAGTGTGGTCTAGCAAATCTAAATGGCATTCATCACTTCTCT
  GGAAAATTGCTTGCAACTGGAATTCAATGGGGCACGTGGACCAAAAACAACTCACCTGTCAAGATTAAAT
  CTGTTTCAATGAAAATTAGAAGAATGTACAATCCATATTTTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208478 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMSPSQASLLFLNVCIFICGEAVQGNCVHHSTDSSVVNIVEDGSNAKDESKSNDTVCKEDCEESCDVKTK
  ITREEKHFMCRNLQNSIVSYTRSTKKLLRNMMDEQQASLDYLSNQVNELMNRVLLLTTEVFRKQLDPFPH
  RPVQSHGLDCTDIKDTIGSVTKTPSGLYIIHPEGSSYPFEVMCDMDYRGGGWTVIQKRIDGIIDFQRLWC
  DYLDGFGDLLGEFWLGLKKIFYIVNQKNTSFMLYVALESEDDTLAYASYDNFWLEDETRFFKMHLGRYSG
  NAGDAFRGLKKEDNQNAMPFSTSDVDNDGCRPACLVNGQSVKSCSHLHNKTGWWFNECGLANLNGIHHFS
  GKLLATGIQWGTWTKNNSPVKIKSVSMKIRRMYNPYFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178127
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178127.3
• RefSeq Size: 2383 bp
• RefSeq ORF: 1167 bp
• Locus ID: 253935
• UniProt ID: Q86XS5
• Cytogenetics: 11q22.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• MW: 44.2 kDa