R3HDM1 (NM_015361) Human Tagged ORF Clone

CAT#: RC207750

R3HDM1 (Myc-DDK-tagged)-Human R3H domain containing 1 (R3HDM1)

Product Images

Circular map for RC207750

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: R3HDM1
• Synonyms: R3HDM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207750 representing NM_015361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATGTCTGATACTGTTACTGTAAAAGATGAAACTGCAACAATGAAGGATTTGGAGGCAGAAGTGA
  AAGATACAACCAGAGTTGAAAATCTTATCAAATCAGAAAACTATGGGAAGATTTTGGTAGAGAAGAATGA
  ACATTGTATTGAGAACAATATAGATTTGCAGCGGCCATTGCAGTCATTTGGACAGACAGGAAAAAGGTCT
  AAGTCAAGCTCAAAGTTAAAGCTAGTTCGGAGCCTTGCAGTGTGTGAAGAATCTCCACCACCCCCTGCAC
  CAGAGATATCACAGGAGAACCAGGAGAAAATTCAGATCCAGTTAACACAATCATTTGAGAAAGAAGAGAA
  GCCCTCAAAAGATGAAGCAGAAAAAGAAAAGGCCAGTGATAAGTTGCCCAGAAAAATGTTATCAAGAGAT
  TCCAGTCAAGAATACACTGATTCAACTGGCATAGATCTACATGAATTTTTAGTAAATACATTAAAAAACA
  ATCCCAGGGACAGAATGATGCTGCTGAAATTGGAACAAGAAATTTTAGATTTCATTGGTAATAATGAGTC
  TCCACGTAAAAAATTCCCCCCAATGACATCTTACCATAGGATGCTATTACACAGAGTAGCCGCTTACTTT
  GGATTAGACCACAATGTTGATCAGAGTGGGAAGTCTGTCATAGTAAACAAAACTAGCAATACAAGAATAC
  CTGATCAGAAATTTAATGAACATATTAAGGATGATAAAGGTGAAGACTTTCAGAAACGTTATATCCTCAA
  GAGAGATAACTCTAGCTTTGACAAAGATGATAACCAGATGAGAATACGTTTGAAAGATGACAGAAGAAGC
  AAATCTATAGAAGAAAGAGAAGAAGAGTACCAGAGAGCCAGAGACCGAATATTTTCCCAAGATTCCCTGT
  GTTCCCAAGAGAATTACATTATTGACAAAAGACTCCAAGACGAGGATGCCAGTAGTACCCAGCAGAGGCG
  CCAGATATTTAGAGTTAATAAAGATGCTTCAGGGAGATCTACAAATAGCCATCAAAGCAGCACTGAGAAT
  GAGTTGAAGTACTCGGAACCACGACCCTGGAGCAGCACAGATTCAGACAGCTCTCTTCGAAACCTGAAAC
  CTGCTGTAACCAAAGCCAGCAGCTTCAGTGGAATCTCAGTCCTGACAAGAGGTGATAGTTCTGGAAGCAG
  CAAAAGCATAGGCAGGCTTTCAAAAACAGGTTCTGAGTCTTCTGGTAGTGTAGGGTCATCTACAGGCTCT
  CTTTCTCACATCCAGCAGCCTCTTCCAGGTACAGCTCTCAGCCAGTCTTCTCATGGCGCACCTGTCGTCT
  ATCCAACTGTCAGCACTCATAGTTCTCTTTCCTTTGATGGTGGCCTAAATGGGCAAGTCGCATCTCCTAG
  CACTAGCTTCTTTTTGCTTCCCTTGGAAGCGGCAGGCATACCACCTGGCAGTATTCTGATCAACCCACAA
  ACAGGTCAGCCCTTCATAAACCCAGATGGGAGTCCAGTTGTGTATAATCCTCCTATGACTCAACAACCAG
  TTAGATCCCAAGTGCCTGGACCTCCACAGCCACCTCTGCCAGCCCCACCTCAACAACCAGCAGCTAATCA
  CATTTTCTCACAGGATAACCTAGGGTCTCAGTTTAGCCACATGAGTCTTGCTCGCCAGCCATCTGCTGAT
  GGTTCTGACCCTCATGCCGCCATGTTCCAGTCCACTGTGGTTCTTCAGTCTCCACAGCAGTCTGGTTATA
  TCATGACAGCAGCCCCTCCACCACATCCTCCTCCACCGCCACCACCACCACCTCCTCCTCCTCCCCTACC
  ACCTGGGCAGCCAGTCCCTACTGCTGGATATCCTGCCTCTGGTCATCCTGTCAGCCAGCCTGTGCTCCAG
  CAGCAGGGATATATTCAGCAGCCATCACCACAGATGCCAGCCTGTTATTGCGCTCCAGGCCACTATCACT
  CCAGCCAACCTCAGTATCGCCCAGTCCCTTCTGTTCATTACAATTCACATCTAAACCAACCACTGCCACA
  ACCTGCGCAGCAGACAGGTTATCAAGTTATACCCAACCAGCAGCAAAACTACCAAGGAATAGTTGGAGTT
  CAGCAACCCCAGAGTCAGAGCCTAGTCAGTGGCCAACCCAACAGCATTGGAAATCAGATTCAAGGAGTGG
  TCATCCCCTATACTTCAGTGCCAACATATCAGGTTTCACTGCCTCAAGGTTCTCAAGGAATTCCCCATCA
  GACTTATCAACAGCCTGTTATGTTCCCTAATCAGTCTAATCAAGGATCTATGCCCACAACAGGAATGCCT
  GTTTACTATAGTGTCATTCCACCTGGTCAACAAAACAATTTAAGCTCTTCAGTAGGTTACCTGCAACATC
  CAGGATCAGAACAAGTACAATTTCCTCGAACCACTTCACCATGCAGTTCCCAGCAGCTTCAAGGCCACCA
  ATGTACAGCTGGACCACCACCGCCACCTGGTGGGGGGATGGTGATGATGCAGCTCAGTGTACCAAACAAT
  CCACAATCTTGTGCCCACTCACCCCCGCAGTGGAAACAAAACAAATATTACTGTGATCACCAGAGAGGAC
  AGAAGTGTGTAGAATTTAGCAGTGTAGACAATATTGTCCAGCACAGCCCTCAACTCAGTAGCCCCATTAT
  TTCACCAGCTCAGTCGCCAGCACCAGCTCAGCTGTCCACCCTGAAAACTGTACGTCCCTCTGGACCACCA
  CTTTCCATCATGCCCCAATTTTCTAGACCTTTTGTCCCCGGGCAAGGAGATTCCAGGTATCCATTACTTG
  GCCAGCCACTGCAGTACAATCCTCCTGCTGTTCTGCACGGACACATTCCAAACCAACAGGGTCAGCCTGG
  CAGCAGGCATGGAAACCGAGGAAGGAGACAAGCTAAAAAAGCTGCATCCACAGACCTTGGAGCAGGAGAA
  ACAGTTGTTGGGAAGGTCTTGGAAATTACTGAACTACCAGATGGAATAACTCGCATGGAAGCTGAAAAGC
  TTTTTGGGGAACTCTTTAAAATTGGCGCCAAGATCCGGTGGCTCCGGGACCCCCAGTCCCAACCACGTCG
  TCACCCCCTCTGCTGTGGCAGTGGGGACAACACTGCCAACCCTGAACGCTCTAAACCCAGTGACTTGGCC
  TCCACCTACACCGTCTTAGCCACATTCCCCTCCATTTCAGCTGCACAGAATGCACTGAAGAAACAAATTA
  ACTCAGTTAACAAGTTTAAGCTGAGAACAAGCAAGAAGCACTATGACTTTCACATTTTGGAAAGGGCAAG
  TTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207750 representing NM_015361
  Red=Cloning site Green=Tags(s)
  
  MRMSDTVTVKDETATMKDLEAEVKDTTRVENLIKSENYGKILVEKNEHCIENNIDLQRPLQSFGQTGKRS
  KSSSKLKLVRSLAVCEESPPPPAPEISQENQEKIQIQLTQSFEKEEKPSKDEAEKEKASDKLPRKMLSRD
  SSQEYTDSTGIDLHEFLVNTLKNNPRDRMMLLKLEQEILDFIGNNESPRKKFPPMTSYHRMLLHRVAAYF
  GLDHNVDQSGKSVIVNKTSNTRIPDQKFNEHIKDDKGEDFQKRYILKRDNSSFDKDDNQMRIRLKDDRRS
  KSIEEREEEYQRARDRIFSQDSLCSQENYIIDKRLQDEDASSTQQRRQIFRVNKDASGRSTNSHQSSTEN
  ELKYSEPRPWSSTDSDSSLRNLKPAVTKASSFSGISVLTRGDSSGSSKSIGRLSKTGSESSGSVGSSTGS
  LSHIQQPLPGTALSQSSHGAPVVYPTVSTHSSLSFDGGLNGQVASPSTSFFLLPLEAAGIPPGSILINPQ
  TGQPFINPDGSPVVYNPPMTQQPVRSQVPGPPQPPLPAPPQQPAANHIFSQDNLGSQFSHMSLARQPSAD
  GSDPHAAMFQSTVVLQSPQQSGYIMTAAPPPHPPPPPPPPPPPPPLPPGQPVPTAGYPASGHPVSQPVLQ
  QQGYIQQPSPQMPACYCAPGHYHSSQPQYRPVPSVHYNSHLNQPLPQPAQQTGYQVIPNQQQNYQGIVGV
  QQPQSQSLVSGQPNSIGNQIQGVVIPYTSVPTYQVSLPQGSQGIPHQTYQQPVMFPNQSNQGSMPTTGMP
  VYYSVIPPGQQNNLSSSVGYLQHPGSEQVQFPRTTSPCSSQQLQGHQCTAGPPPPPGGGMVMMQLSVPNN
  PQSCAHSPPQWKQNKYYCDHQRGQKCVEFSSVDNIVQHSPQLSSPIISPAQSPAPAQLSTLKTVRPSGPP
  LSIMPQFSRPFVPGQGDSRYPLLGQPLQYNPPAVLHGHIPNQQGQPGSRHGNRGRRQAKKAASTDLGAGE
  TVVGKVLEITELPDGITRMEAEKLFGELFKIGAKIRWLRDPQSQPRRHPLCCGSGDNTANPERSKPSDLA
  STYTVLATFPSISAAQNALKKQINSVNKFKLRTSKKHYDFHILERASSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015361
• ORF Size: 3297 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015361.4
• RefSeq Size: 4666 bp
• RefSeq ORF: 3300 bp
• Locus ID: 23518
• UniProt ID: Q15032
• Cytogenetics: 2q21.3
• Domains: R3H
• MW: 120.5 kDa