FNDC4 (NM_022823) Human Tagged ORF Clone

CAT#: RC207471

FNDC4 (Myc-DDK-tagged)-Human fibronectin type III domain containing 4 (FNDC4)

Product Images

Circular map for RC207471

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FNDC4 (Cat# RC207471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FNDC4(Cat# TA505462). Positive lysates LY411537 (100ug) and LC411537 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY411537) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207471 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FNDC4 protein (Cat# TP307471). The protein was produced from HEK293T cells transfected with FNDC4 cDNA clone (Cat# RC207471) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNDC4
• Synonyms: FRCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207471 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGCGGATGCCACAGTTCCCCCCCCAGCGGACTCCGTGGGGACATGGCTTCGCTGGTGCCCCTTT
  CCCCATATCTAAGCCCCACGGTCCTCCTGCTGGTCAGCTGTGACCTGGGCTTCGTGCGAGCAGACCGGCC
  TCCCTCTCCTGTGAATGTGACGGTCACTCACCTCAGAGCCAACTCGGCCACTGTGTCCTGGGACGTCCCA
  GAAGGCAACATCGTCATTGGCTACTCCATTTCCCAGCAACGGCAGAATGGCCCCGGGCAGCGTGTGATTC
  GGGAGGTGAACACCACCACCCGGGCCTGTGCCCTCTGGGGCCTGGCTGAAGACAGTGACTACACAGTGCA
  GGTCAGGAGCATCGGCCTTCGGGGAGAGAGTCCCCCAGGGCCCCGGGTGCACTTCCGAACTCTCAAGGGT
  TCTGACCGGCTACCTTCAAACAGTTCAAGCCCAGGTGACATCACAGTGGAAGGTCTGGATGGAGAGCGGC
  CACTGCAGACTGGGGAAGTGGTCATCATTGTGGTGGTGTTGCTCATGTGGGCTGCTGTAATTGGGCTGTT
  CTGCCGTCAGTATGACATCATCAAGGACAATGACTCCAACAACAATCCCAAGGAGAAGGGAAAGGGGCCG
  GAACAGAGTCCTCAGGGAAGGCCAGTGGGGACAAGACAGAAAAAGTCACCATCTATCAACACCATCGACG
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207471 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVP
  EGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKG
  SDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGP
  EQSPQGRPVGTRQKKSPSINTIDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022823
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022823.3
• RefSeq Size: 1697 bp
• RefSeq ORF: 705 bp
• Locus ID: 64838
• UniProt ID: Q9H6D8
• Cytogenetics: 2p23.3
• Domains: FN3
• Protein Families: Transmembrane
• MW: 25.2 kDa
• Gene Summary: Acts as an anti-inflammatory factor in the intestine and colon. Binds to and acts on macrophages to downregulate pro-inflammatory gene expression. Affects key macrophage functions, including phagocytosis, by downregulating many key pathways for macrophage activation, partly via by STAT3 activation and signaling. May be required to dampen the immunological response in colitis.[UniProtKB/Swiss-Prot Function]