Centaurin gamma 1 (AGAP2) (NM_014770) Human Tagged ORF Clone
CAT#: RC206993
- TrueORF®
AGAP2 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_014770" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Centaurin gamma 1 |
Synonyms | CENTG1; GGAP2; PIKE |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGCCCAGAGGCAGTTCGTTGTAGCTGCAGTGAGAGCAGAAGTCAGACGACATGAGGTGGCCAAGC AGGCTCTAAACCGCCTCAGGAAGCTGGCAGAGAGGGTGGACGACCCCGAACTCCAGGACAGCATCCAGGC CTCATTGGACAGCATTCGAGAGGCTGTGATCAATAGCCAGGAATGGACTTTGAGCCGCTCCATTCCTGAA CTGCGCCTGGGTGTGCTGGGCGATGCCAGGAGTGGGAAGTCATCGCTCATCCACCGATTCCTGACTGGCT CATACCAGGTGCTGGAGAAGACAGAGAGTGAGCAGTACAAGAAAGAAATGTTGGTGGATGGACAGACACA TCTGGTGCTAATCCGAGAGGAAGCTGGGGCACCTGATGCCAAGTTCTCAGGCTGGGCAGATGCTGTGATC TTCGTCTTCAGCCTGGAGGATGAGAACAGTTTCCAGGCTGTGAGCCGTCTCCATGGGCAGCTGAGTTCCC TTCGCGGGGAGGGACGAGGAGGCCTGGCCTTGGCACTGGTGGGGACACAAGACAGGATCAGTGCTTCCTC CCCTCGGGTGGTGGGAGATGCTCGTGCCAGAGCTCTGTGCGCGGACATGAAACGCTGCAGCTACTATGAG ACTTGTGCAACCTATGGGCTCAATGTGGATCGGGTCTTCCAGGAGGTGGCCCAGAAGGTGGTGACCTTGC GCAAGCAGCAACAGCTTCTGGCTGCCTGCAAGTCCCTTCCCAGCTCCCCAAGCCACTCAGCTGCATCCAC TCCGGTAGCTGGCCAGGCTAGTAACGGGGGCCACACTAGCGACTACTCTTCTTCCCTCCCGTCCTCACCG AATGTTGGTCACCGGGAGCTCCGAGCCGAGGCAGCTGCAGTGGCTGGATTGAGCACCCCAGGGTCCCTGC ACCGGGCAGCCAAGCGCAGGACCAGCCTTTTTGCGAATCGTCGGGGTAGTGACTCCGAGAAACGAAGCTT GGATAGTCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCTACTAAAACGA AGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTAACCCTGTCCAGTAATGGCTTTCTACTCT ACCACCCCAGTATTAACGATTACATCCACAGTACCCACGGCAAGGAGATGGACTTGCTGCGAACAACAGT CAAAGTCCCGGGCAAGCGGCCCCCGAGGGCCATCTCTGCCTTTGGCCCCTCAGCCAGCATTAACGGGCTC GTCAAGGACATGAGCACTGTCCAGATGGGTGAAGGCCTGGAAGCCACTACTCCCATGCCAAGCCCTAGCC CCAGCCCCAGTTCCCTGCAGCCACCACCAGATCAGACATCCAAACACCTGCTGAAGCCAGACCGGAATTT GGCCCGAGCCCTCAGCACGGACTGTACCCCATCTGGAGACCTGAGCCCCCTGAGTCGGGAACCCCCTCCT TCTCCCATGGTGAAGAAGCAGAGGAGGAAAAAATTGACAACACCATCCAAGACTGAAGGCTCGGCTGGGC AGGCTGAAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACGGGTCAGACGTGGCACTTTGAGGCAGC CAGTTTTGAGGAGCGGGATGCCTGGGTCCAGGCCATCGAGAGTCAGATCCTAGCCAGTCTGCAATGCTGT GAGAGCAGCAAGGTCAAGCTGCGCACAGACAGCCAAAGCGAGGCCGTGGCCATCCAGGCGATCCGGAACG CCAAGGGGAATTCAATCTGCGTGGACTGCGGGGCCCCCAACCCCACGTGGGCCAGCTTGAACCTGGGCGC CCTCATCTGCATCGAGTGTTCTGGCATCCACCGCAACCTGGGCACACACCTGTCCCGCGTTCGCTCGCTG GACTTGGACGACTGGCCACGGGAGCTGACCCTGGTGCTGACGGCTATTGGCAACGACACGGCCAACCGCG TGTGGGAAAGCGACACGCGAGGCCGTGCCAAGCCCTCGCGGGACTCTTCGCGGGAGGAGCGCGAGTCGTG GATTCGCGCCAAGTACGAGCAGCTACTGTTCCTGGCGCCGCTGAGCACCTCGGAGGAGCCGCTGGGCCGC CAGCTGTGGGCCGCCGTGCAGGCCCAGGACGTGGCTACCGTTCTCCTGCTTTTGGCCCATGCGCGACACG GGCCGCTCGACACCAGCGTAGAGGACCCACAGCTGCGCTCCCCACTCCACCTGGCGGCCGAGCTCGCCCA CGTCGTCATCACGCAACTGCTGCTGTGGTACGGCGCGGACGTGGCGGCCCGTGACGCCCAGGGCCGCACG GCGCTGTTCTACGCCCGCCAGGCTGGAAGCCAGCTGTGCGCCGACATCCTTCTCCAGCACGGCTGCCCGG GTGAGGGCGGCAGCGCGGCCACCACGCCCAGCGCGGCCACCACGCCCAGCATCACCGCCACGCCCAGCCC CCGCCGCCGGAGCAGCGCCGCTAGCGTGGGCCGCGCCGACGCCCCGGTTGCGCTGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206993 protein sequence
Red=Cloning site Green=Tags(s) MHAQRQFVVAAVRAEVRRHEVAKQALNRLRKLAERVDDPELQDSIQASLDSIREAVINSQEWTLSRSIPE LRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVI FVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYE TCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSP NVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKR SGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGL VKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPP SPMVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCC ESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSL DLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGR QLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRT ALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014770 |
ORF Size | 2508 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014770.4 |
RefSeq Size | 4860 bp |
RefSeq ORF | 2511 bp |
Locus ID | 116986 |
UniProt ID | Q99490 |
Cytogenetics | 12q14.1 |
Domains | ArfGap, PH, ANK, RAS, RAB |
Protein Pathways | Endocytosis |
MW | 90.5 kDa |
Gene Summary | The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206993L3 | Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, Myc-DDK-tagged |
USD 1,448.00 |
|
RC206993L4 | Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mGFP tagged |
USD 1,448.00 |
|
RG206993 | AGAP2 (tGFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2 |
USD 1,348.00 |
|
SC114863 | AGAP2 (untagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2 |
USD 1,149.00 |
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