C2orf18 (SLC35F6) (NM_017877) Human Tagged ORF Clone

CAT#: RC206986

SLC35F6 (Myc-DDK-tagged)-Human chromosome 2 open reading frame 18 (C2orf18)

Product Images

Circular map for RC206986

• 

  Western blot validation of overexpression lysate (Cat# LY413498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206986 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C2orf18
• Synonyms: ANT2BP; C2orf18; TANGO9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206986 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGACCAAGTACCAGCTGTTCCTGGCCGGGCTCATGCTTGTTACCGGCTCCATCAACACGCTCT
  CGGCAAAATGGGCGGACAATTTCATGGCCGAGGGCTGTGGAGGGAGCAAGGAGCACAGCTTCCAGCATCC
  CTTCCTCCAGGCAGTGGGCATGTTCCTGGGAGAATTCTCCTGCCTGGCTGCCTTCTACCTCCTCCGATGC
  AGAGCTGCAGGGCAATCAGACTCCAGCGTAGACCCCCAGCAGCCCTTCAACCCTCTTCTTTTCCTGCCCC
  CAGCGCTCTGTGACATGACAGGGACCAGCCTCATGTATGTGGCTCTGAACATGACCAGTGCCTCCAGCTT
  CCAGATGCTGCGGGGTGCAGTGATCATATTCACTGGCCTGTTCTCGGTGGCCTTCCTGGGCCGGAGGCTG
  GTGCTGAGCCAGTGGCTGGGCATCCTAGCCACCATCGCGGGGCTGGTGGTCGTGGGCCTGGCTGACCTCC
  TGAGCAAGCACGACAGTCAGCACAAGCTCAGCGAAGTGATCACAGGGGACCTGTTGATCATCATGGCCCA
  GATCATCGTTGCCATCCAGATGGTGCTAGAGGAGAAGTTCGTCTACAAACACAATGTGCACCCACTGCGG
  GCAGTTGGCACTGAGGGCCTCTTTGGCTTTGTGATCCTCTCCCTGCTGCTGGTGCCCATGTACTACATCC
  CCGCCGGCTCCTTCAGCGGAAACCCTCGTGGGACACTGGAGGATGCATTGGACGCCTTCTGCCAGGTGGG
  CCAGCAGCCGCTCATTGCCGTGGCACTGCTGGGCAACATCAGCAGCATTGCCTTCTTCAACTTCGCAGGC
  ATCAGCGTCACCAAGGAACTGAGCGCCACCACCCGCATGGTGTTGGACAGCTTGCGCACCGTTGTCATCT
  GGGCACTGAGCCTGGCACTGGGCTGGGAGGCCTTCCATGCACTGCAGATCCTTGGCTTCCTCATACTCCT
  TATAGGCACTGCCCTCTACAATGGGCTACACCGTCCGCTGCTGGGCCGCCTGTCCAGGGGCCGGCCCCTG
  GCAGAGGAGAGCGAGCAGGAGAGACTGCTGGGTGGCACCCGCACTCCCATCAATGATGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206986 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAWTKYQLFLAGLMLVTGSINTLSAKWADNFMAEGCGGSKEHSFQHPFLQAVGMFLGEFSCLAAFYLLRC
  RAAGQSDSSVDPQQPFNPLLFLPPALCDMTGTSLMYVALNMTSASSFQMLRGAVIIFTGLFSVAFLGRRL
  VLSQWLGILATIAGLVVVGLADLLSKHDSQHKLSEVITGDLLIIMAQIIVAIQMVLEEKFVYKHNVHPLR
  AVGTEGLFGFVILSLLLVPMYYIPAGSFSGNPRGTLEDALDAFCQVGQQPLIAVALLGNISSIAFFNFAG
  ISVTKELSATTRMVLDSLRTVVIWALSLALGWEAFHALQILGFLILLIGTALYNGLHRPLLGRLSRGRPL
  AEESEQERLLGGTRTPINDAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017877
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017877.4
• RefSeq Size: 3912 bp
• RefSeq ORF: 1116 bp
• Locus ID: 54978
• UniProt ID: Q8N357
• Cytogenetics: 2p23.3
• Protein Families: Transmembrane
• MW: 40.2 kDa
• Gene Summary: Involved in the maintenance of mitochondrial membrane potential in pancreatic ductal adenocarcinoma (PDAC) cells. Promotes pancreatic ductal adenocarcinoma (PDAC) cell growth. May play a role as a nucleotide-sugar transporter.[UniProtKB/Swiss-Prot Function]