APBB2 (NM_173075) Human Tagged ORF Clone

CAT#: RC206803

APBB2 (Myc-DDK-tagged)-Human amyloid beta (A4) precursor protein-binding, family B, member 2 (APBB2), transcript variant 3

Product Images

Circular map for RC206803

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APBB2
• Synonyms: FE65L; FE65L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206803 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAAGTACTTCCAGCTGACTCAGGTGTTGACACCTTGGCAGTGTTTATGGCCAGCAGCGGAACTA
  CAGACGTCACAAATCGGAACAGCCCAGCCACACCACCAAACACCCTTAACCTCCGATCCTCCCACAATGA
  ACTGTTGAACGCTGAAATAAAACACACAGAAACCAAGAACAGCACACCTCCCAAATGCAGGAAAAAATAT
  GCACTAACTAACATCCAGGCGGCCATGGGCCTCTCGGATCCAGCTGCACAGCCCCTGCTGGGAAATGGCT
  CTGCCAACATCAAGCTGGTGAAAAATGGGGAGAACCAGCTCCGTAAGGCTGCAGAGCAAGGGCAGCAGGA
  CCCCAACAAAAACCTGAGCCCCACTGCAGTCATCAACATAACTTCTGAGAAGTTAGAGGGTAAAGAGCCC
  CACCCACAGGATTCCTCGAGCTGTGAGATTTTACCCTCCCAGCCCAGGAGAACTAAGAGCTTCCTAAATT
  ACTATGCAGATCTGGAAACCTCAGCCAGAGAACTAGAGCAGAACCAAGGCAATCACCATGGGACTGCGGA
  AGAGAAATCCCAGCCAGTCCAGGGCCAGGCCTCCACCATCATTGGGAATGGTGATTTGCTGCTGCAGAAA
  CCAAACAGACCCCAGTCCAGCCCTGAAGACGGCCAAGTAGCCACAGTGTCATCCAGCCCAGAAACCAAGA
  AGGATCATCCGAAAACAGGGGCCAAAACCGACTGTGCACTGCACCGGATCCAGAACCTGGCACCGAGCGA
  TGAGGAGTCCAGCTGGACAACGTTGTCCCAAGACAGTGCCTCACCCAGCTCCCCGGATGAAACAGATATA
  TGGAGTGATCACTCATTTCAGACTGATCCAGATTTGCCGCCTGGCTGGAAAAGAGTCAGTGACATTGCCG
  GGACCTATTATTGGCACATCCCAACAGGAACGACTCAGTGGGAACGGCCCGTCTCCATCCCAGCAGATCT
  CCAGGGTTCTAGGAAAGGGTCACTTAGTTCTGTAACGCCATCTCCCACCCCAGAGAACGAGGATTTGCAT
  GCAGCCACTGTTAACCCGGACCCCAGTTTAAAAGAGTTTGAAGGAGCAACCCTACGCTATGCATCTTTGA
  AACTCAGAAATGCCCCACACCCTGATGATGATGATTCTTGTAGTATCAACAGTGACCCAGAAGCCAAGTG
  TTTTGCTGTGCGTTCTCTGGGATGGGTAGAGATGGCAGAAGAGGACCTCGCCCCCGGTAAAAGTAGTGTT
  GCGGTCAACAACTGCATCAGGCAACTTTCCTACTGCAAAAATGACATCCGAGACACAGTCGGGATTTGGG
  GAGAGGGGAAAGACATGTACCTGATCCTGGAGAATGACATGCTCAGCCTGGTGGACCCCATGGACCGCAG
  CGTGCTGCACTCGCAGCCCATCGTCAGCATCCGCGTGTGGGGCGTGGGCCGCGACAATGGCCGGGATTTT
  GCTTATGTAGCAAGAGATAAAGATACAAGAATTTTGAAATGTCATGTATTTCGATGTGACACACCAGCAA
  AAGCCATTGCCACAAGTCTCCACGAGATCTGCTCCAAGATTATGGCTGAACGGAAGAATGCCAAAGCGCT
  GGCCTGCAGCTCCTTACAGGAAAGGGCCAATGTGAACCTCGATGTCCCTTTGCAAGATTTTCCAACACCA
  AAGACTGAGCTGGTCCAGAAGTTCCACGTGCAGTACTTGGGCATGTTACCTGTAGACAAACCAGTCGGAA
  TGGATATTTTGAACAGTGCCATAGAAAATCTTATGACCTCATCCAACAAGGAGGACTGGCTGTCAGTGAA
  CATGAACGTGGCTGATGCCACTGTGACTGTCATCAGTGAAAAGAATGAAGAGGAAGTCTTAGTGGAATGT
  CGTGTGCGATTCCTGTCCTTCATGGGTGTTGGGAAGGACGTCCACACATTTGCCTTCATCATGGACACGG
  GGAACCAGCGCTTTGAGTGCCACGTTTTCTGGTGCGAGCCTAATGCTGGTAACGTGTCTGAGGCGGTGCA
  GGCCGCCTGCATGTTACGATATCAGAAGTGCTTGGTAGCCAGGCCGCCTTCTCAGAAAGTTCGACCACCT
  CCACCGCCAGCAGATTCAGTAACCAGAAGAGTCACAACCAATGTAAAACGAGGGGTCTTATCCCTCATTG
  ACACTTTGAAACAGAAACGCCCTGTCACCGAAATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206803 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEVLPADSGVDTLAVFMASSGTTDVTNRNSPATPPNTLNLRSSHNELLNAEIKHTETKNSTPPKCRKKY
  ALTNIQAAMGLSDPAAQPLLGNGSANIKLVKNGENQLRKAAEQGQQDPNKNLSPTAVINITSEKLEGKEP
  HPQDSSSCEILPSQPRRTKSFLNYYADLETSARELEQNQGNHHGTAEEKSQPVQGQASTIIGNGDLLLQK
  PNRPQSSPEDGQVATVSSSPETKKDHPKTGAKTDCALHRIQNLAPSDEESSWTTLSQDSASPSSPDETDI
  WSDHSFQTDPDLPPGWKRVSDIAGTYYWHIPTGTTQWERPVSIPADLQGSRKGSLSSVTPSPTPENEDLH
  AATVNPDPSLKEFEGATLRYASLKLRNAPHPDDDDSCSINSDPEAKCFAVRSLGWVEMAEEDLAPGKSSV
  AVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLILENDMLSLVDPMDRSVLHSQPIVSIRVWGVGRDNGRDF
  AYVARDKDTRILKCHVFRCDTPAKAIATSLHEICSKIMAERKNAKALACSSLQERANVNLDVPLQDFPTP
  KTELVQKFHVQYLGMLPVDKPVGMDILNSAIENLMTSSNKEDWLSVNMNVADATVTVISEKNEEEVLVEC
  RVRFLSFMGVGKDVHTFAFIMDTGNQRFECHVFWCEPNAGNVSEAVQAACMLRYQKCLVARPPSQKVRPP
  PPPADSVTRRVTTNVKRGVLSLIDTLKQKRPVTEMP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173075
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173075.5
• RefSeq Size: 8935 bp
• RefSeq ORF: 2211 bp
• Locus ID: 323
• UniProt ID: Q92870
• Cytogenetics: 4p14-p13
• Protein Families: Transcription Factors
• MW: 80.9 kDa
• Gene Summary: The protein encoded by this gene interacts with the cytoplasmic domains of amyloid beta (A4) precursor protein and amyloid beta (A4) precursor-like protein 2. This protein contains two phosphotyrosine binding (PTB) domains, which are thought to function in signal transduction. Polymorphisms in this gene have been associated with Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]