CABP (CABP1) (NM_001033677) Human Tagged ORF Clone

CAT#: RC206637

CABP1 (Myc-DDK-tagged)-Human calcium binding protein 1 (CABP1), transcript variant 3

Product Images

Circular map for RC206637

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CABP
• Synonyms: CALBRAIN; HCALB_BR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206637 representing NM_001033677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCGGCGACGGGGCCGCATTTAAGCGGCCGGGGGACGGCGCCCGCCTCCAGCGCGTCCTCGGGC
  TTGGCTCCCGCCGGGAGCCCCGTTCTCTGCCCGCCGGGGGCCCCGCGCCGCGCCGCACCGCGCCGCCCCC
  GCCGGGCCATGCGAGCGCGGGCCCCGCCGCGATGAGCTCGCACATCGCCAAAAGCGAGTCCAAGACGTCG
  CTGCTGAAGGCGGCGGCGGCGGCGGCGAGCGGGGGCAGCCGGGCTCCCCGCCACGGCCCTGCCCGGGACC
  CGGGGCTGCCTAGCCGCCGGCTACCCGGCTCCTGCCCGGCGACGCCGCAGTCGTCCGGGGACCCCAGTTC
  GCGGAGGCCCCTGTGCCGGCCGGCGCCGCGAGAGGAGGGCGCGCGGGGGAGCCAGCGTGTGCTCCCCCGG
  GCGCACTGCAGGCCCCGGGAGGCGCTGCCGGCCGCGGCGTCCCGACCTTCGCCGTCGTCGCCGCTGCCGC
  CGGCCCGCGGGCGGGATGGGGAGGAACGGGGACTGTCCCCGGCGCTCGGCCTCCGGGGCTCTCTGCGAGC
  CCGGGGCCGCGGGGACTCCGTTCCAGCCGCCGCGTCCGAGGCGGACCCGTTCCTCCACCGGCTGCGCCCC
  ATGCTCAGCTCCGCCTTTGGCCAGGATAGATCACTGCGACCAGAGGAAATTGAAGAGCTCCGAGAGGCCT
  TCAGAGAATTCGACAAGGACAAGGATGGCTACATCAACTGCCGGGATCTGGGCAACTGCATGCGCACCAT
  GGGCTACATGCCCACCGAGATGGAGCTCATCGAACTGTCCCAGCAGATCAACATGAACCTGGGTGGCCAT
  GTAGATTTTGATGACTTCGTGGAGCTAATGGGGCCTAAACTCCTGGCAGAGACAGCAGATATGATTGGTG
  TAAAGGAACTGCGAGATGCTTTCCGAGAGTTTGACACCAATGGTGATGGGGAAATAAGCACCAGTGAGCT
  GCGAGAGGCTATGAGGAAGCTCCTGGGTCATCAGGTGGGACACCGAGACATAGAGGAAATTATCCGAGAT
  GTGGACCTCAATGGGGATGGACGAGTGGACTTTGAAGAGTTTGTCCGGATGATGTCCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206637 representing NM_001033677
  Red=Cloning site Green=Tags(s)
  
  MGGGDGAAFKRPGDGARLQRVLGLGSRREPRSLPAGGPAPRRTAPPPPGHASAGPAAMSSHIAKSESKTS
  LLKAAAAAASGGSRAPRHGPARDPGLPSRRLPGSCPATPQSSGDPSSRRPLCRPAPREEGARGSQRVLPR
  AHCRPREALPAAASRPSPSSPLPPARGRDGEERGLSPALGLRGSLRARGRGDSVPAAASEADPFLHRLRP
  MLSSAFGQDRSLRPEEIEELREAFREFDKDKDGYINCRDLGNCMRTMGYMPTEMELIELSQQINMNLGGH
  VDFDDFVELMGPKLLAETADMIGVKELRDAFREFDTNGDGEISTSELREAMRKLLGHQVGHRDIEEIIRD
  VDLNGDGRVDFEEFVRMMSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033677
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033677.1, NP_001028849.1
• RefSeq Size: 1627 bp
• RefSeq ORF: 1113 bp
• Locus ID: 9478
• UniProt ID: Q9NZU7
• Cytogenetics: 12q24.31
• MW: 39.7 kDa
• Gene Summary: Calcium binding proteins are an important component of calcium mediated cellular signal transduction. This gene encodes a protein that belongs to a subfamily of calcium binding proteins which share similarity to calmodulin. The protein encoded by this gene regulates the gating of voltage-gated calcium ion channels. This protein inhibits calcium-dependent inactivation and supports calcium-dependent facilitation of ion channels containing voltage-dependent L-type calcium channel subunit alpha-1C. This protein also regulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors, P/Q-type voltage-gated calcium channels, and transient receptor potential channel TRPC5. This gene is predominantly expressed in retina and brain. Alternative splicing results in multiple transcript variants encoding disinct isoforms. [provided by RefSeq, Jul 2012]