MFSD4B (NM_153369) Human Tagged ORF Clone

CAT#: RC206353

KIAA1919 (Myc-DDK-tagged)-Human KIAA1919 (KIAA1919)

Product Images

Circular map for RC206353

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MFSD4B
• Synonyms: KIAA1919; NaGLT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206353 representing NM_153369
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGTGCCTCCTTCCTGGGGCTGGGATTGAGTGTTGCTATAGTGGGACCCACGTTTCAAGATTTGG
  CAACAAACGTGAACCGAAATATCAGTAGTCTGTCTTTCATTTTTGTGGGTCGTGCCTTGGGATATTTGAG
  TGGCTCTGTGATTGGTGGATTTCTTGTCGATGTCATGAATTATTTTTTACTTTTGGGAATCTCAATGTCG
  GCTACCACCGTTGGTCTTTATCTTGTTCCTTTTTGCAAGACAGCAATATTACTCACTGTCATGATGTCTA
  TCTTCGGTGTTTCAATTGGCATTCTGGATACAGGTGGTAACGTCCTTATCTTGGCTATTTGGGGGGACAA
  AGGAGCCCCACATATGCAGGCCTTACACTTCTCTTTTGCCTTGGGTGCCTTTTTGGCTCCACTGCTAGCT
  AAACTGGCTTTGGGTCCGACAGCGTCTGCTGAAAACCACACAGAGTCTGACTTCCATCCTGCACTCAACC
  AATCATCTGATGCTGACTCAGAAGCTCTGTTTGGAGTACCTAATGATAAGAATTTACTGTGGGCTTATGC
  TGTTATCGGTACTTACATGTTCTTAGTTTCTGTCATTTTTTTTTGTCTGTTTTTAAAGAATAGCTCAAAG
  CAAGAAAAAGCAAGAGCATCTGCTGAGACATTTCGAAGAGCAAAATATCACAACGCCCTTCTTTGTCTCC
  TTTTTCTGTTCTTCTTTTTTTATGTTGGAGCTGAGGTAACATATGGCTCTTATGTTTTCTCATTTGCAAC
  CACCCATGCTGGCATGAAAGAAAGTGAAGCTGCTGGGTTGAACTCCATCTTCTGGGGGACATTTGCAGCC
  TGCAGGGGCCTGGCAATCTTTTTTGCTACCTGTTTACAGCCTGGAACCATGATTGTGTTGAGCAACATTG
  GCAGCCTGACTTCATCTTTATTTCTGGTGCTTTTTGACAAGAACCCAATTTGTCTCTGGATAGCAACTTC
  AGTGTATGGGGCTTCAATGGCAACCACATTTCCGAGTGGTGTTTCTTGGATTGAGCAGTACACGACCATC
  CATGGGAAATCTGCAGCATTTTTTGTAATTGGTGCTTCCCTGGGAGAAATGGCTATTCCTGCAGTCATTG
  GAATTCTTCAAGGAAAATACCCTGATTTGCCTGTAGTTCTGTATACCTCTTTGGGAGCATCAATAGCTAC
  TGGTATTTTATTTCCTGTGCTATATAAATTAGCCACTTCACCTCTTGATCGCCAGCGAAAAGAAGACAGA
  AAGAGTGAGGACCAGAAAGCTCTGCTCTCTAGCTCCGGGCTAAATGAATATGAGGAAGAGAATGAAGAGG
  AGGATGCAGAAAAATGGAATGAAATGGATTTTGAAATGATTGAAACGAATGATACAATGAGGCATTCTAT
  AATAGAGACATCTAGAAGTAGTCTGACGGAGCCCACAGCTGAAGTCTATAATCAATACCCATCAAATGCA
  CTGGTGTTTGAGTCTTCTCCTTTTAATACTGGCAGTGCCCATGTGAAGCACTTGCCAGAAACCAGGACAA
  AAGGGACTAACGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206353 representing NM_153369
  Red=Cloning site Green=Tags(s)
  
  MLCASFLGLGLSVAIVGPTFQDLATNVNRNISSLSFIFVGRALGYLSGSVIGGFLVDVMNYFLLLGISMS
  ATTVGLYLVPFCKTAILLTVMMSIFGVSIGILDTGGNVLILAIWGDKGAPHMQALHFSFALGAFLAPLLA
  KLALGPTASAENHTESDFHPALNQSSDADSEALFGVPNDKNLLWAYAVIGTYMFLVSVIFFCLFLKNSSK
  QEKARASAETFRRAKYHNALLCLLFLFFFFYVGAEVTYGSYVFSFATTHAGMKESEAAGLNSIFWGTFAA
  CRGLAIFFATCLQPGTMIVLSNIGSLTSSLFLVLFDKNPICLWIATSVYGASMATTFPSGVSWIEQYTTI
  HGKSAAFFVIGASLGEMAIPAVIGILQGKYPDLPVVLYTSLGASIATGILFPVLYKLATSPLDRQRKEDR
  KSEDQKALLSSSGLNEYEEENEEEDAEKWNEMDFEMIETNDTMRHSIIETSRSSLTEPTAEVYNQYPSNA
  LVFESSPFNTGSAHVKHLPETRTKGTNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153369
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153369.4
• RefSeq Size: 3930 bp
• RefSeq ORF: 1557 bp
• Locus ID: 91749
• UniProt ID: Q5TF39
• Cytogenetics: 6q21
• Protein Families: Transmembrane
• MW: 56.2 kDa
• Gene Summary: May function as a sodium-dependent glucose transporter. Potential channels for urea in the inner medulla of kidney.[UniProtKB/Swiss-Prot Function]