Synaptotagmin 4 (SYT4) (NM_020783) Human Tagged ORF Clone

CAT#: RC206304

SYT4 (Myc-DDK-tagged)-Human synaptotagmin IV (SYT4)

Product Images

Circular map for RC206304

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SYT4 (Cat# RC206304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYT4(Cat# TA505862). Positive lysates LY412338 (100ug) and LC412338 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412338) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206304 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SYT4 protein (Cat# TP306304). The protein was produced from HEK293T cells transfected with SYT4 cDNA clone (Cat# RC206304) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synaptotagmin 4
• Synonyms: HsT1192
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206304 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCGATCACCACCAGCCGGGAAGAATTTGATGAAATCCCCACAGTGGTGGGGATCTTCAGTGCAT
  TTGGCCTGGTCTTCACAGTCTCTCTCTTTGCATGGATCTGCTGTCAGAGAAAATCATCCAAGTCTAACAA
  GACTCCTCCATACAAGTTTGTGCATGTGCTTAAGGGAGTTGATATTTACCCTGAAAACCTAAATAGCAAA
  AAGAAGTTTGGAGCAGATGATAAAAATGAAGTAAAGAATAAGCCAGCTGTGCCAAAGAATTCATTGCATC
  TGGATCTTGAAAAGAGAGATCTCAATGGCAATTTTCCCAAAACCAACCTCAAACCTGGCAGTCCTTCTGA
  TCTGGAGAATGCAACCCCGAAGCTCTTTTTAGAAGGGGAAAAAGAGTCAGTTTCCCCTGAGAGTTTAAAG
  TCCAGCACTTCCCTTACTTCAGAAGAGAAACAAGAGAAGCTGGGAACTCTCTTCTTCTCCTTAGAATACA
  ACTTCGAGAGAAAAGCATTTGTGGTCAATATCAAGGAAGCCCGTGGCTTGCCAGCCATGGATGAGCAGTC
  GATGACCTCTGACCCATATATCAAAATGACGATCCTCCCAGAGAAGAAGCATAAAGTGAAAACTAGAGTG
  CTGAGAAAAACCTTGGATCCAGCTTTTGATGAGACCTTTACATTCTATGGGATACCCTACACACAAATCC
  AAGAATTGGCCTTGCACTTCACAATTTTGAGTTTTGACAGGTTTTCAAGAGATGATATCATTGGGGAAGT
  TCTAATTCCTCTCTCGGGAATTGAATTATCTGAAGGAAAAATGTTAATGAATAGAGAGATCATCAAGAGA
  AATGTTAGGAAGTCTTCAGGACGGGGTGAGTTACTGATCTCTCTCTGCTATCAGTCCACCACAAACACTC
  TAACTGTGGTTGTCTTAAAAGCTCGACATCTGCCTAAATCTGATGTGTCCGGACTTTCAGATCCCTATGT
  CAAAGTGAACCTGTACCATGCCAAAAAGAGAATCTCCAAGAAGAAGACTCATGTGAAGAAATGCACCCCC
  AATGCAGTGTTCAATGAGCTGTTTGTCTTTGATATTCCTTGTGAGGGCCTTGAAGATATAAGTGTTGAAT
  TTTTGGTTTTGGATTCTGAAAGGGGGTCCCGAAATGAGGTAATCGGGCAGTTAGTCTTGGGTGCAGCAGC
  AGAAGGAACTGGTGGAGAGCACTGGAAAGAGATCTGTGACTACCCCAGGAGACAAATTGCCAAGTGGCAC
  GTGCTCTGTGATGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206304 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPITTSREEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSK
  KKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLK
  SSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRV
  LRKTLDPAFDETFTFYGIPYTQIQELALHFTILSFDRFSRDDIIGEVLIPLSGIELSEGKMLMNREIIKR
  NVRKSSGRGELLISLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTP
  NAVFNELFVFDIPCEGLEDISVEFLVLDSERGSRNEVIGQLVLGAAAEGTGGEHWKEICDYPRRQIAKWH
  VLCDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020783
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020783.2, NP_065834.1
• RefSeq Size: 4111 bp
• RefSeq ORF: 1278 bp
• Locus ID: 6860
• UniProt ID: Q9H2B2
• Cytogenetics: 18q12.3
• Domains: C2
• Protein Families: Secreted Protein, Transmembrane
• MW: 48 kDa
• Gene Summary: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003).[UniProtKB/Swiss-Prot Function]