ACTRT2 (NM_080431) Human Tagged ORF Clone

CAT#: RC206098

ACTRT2 (Myc-DDK-tagged)-Human actin-related protein T2 (ACTRT2)

Product Images

Circular map for RC206098

• 

  Western blot validation of overexpression lysate (Cat# LY409174) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206098 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ACTRT2 protein (Cat# TP306098). The protein was produced from HEK293T cells transfected with ACTRT2 cDNA clone (Cat# RC206098) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTRT2
• Synonyms: Arp-T2; ARPM2; ARPT2; HARPM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206098 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAATCCGCATGCTTTAGACTCCCCGGCTGTGATTTTTGACAATGGCTCGGGGTTCTGCAAAGCGG
  GCCTGTCTGGGGAGTTTGGACCCCGGCACATGGTCAGCTCCATCGTGGGGCACCTGAAATTCCAGGCTCC
  CTCAGCAGAGGCCAACCAGAAGTACTTTGTGGGGGAGGAGGCCCTGTACAAGCAGGAGGCCCTGCAGCTG
  CACTCCCCTTTCGAGCGTGGCCTGATCACAGGGTGGGATGACGTGGAGAGACTCTGGAAGCACCTCTTTG
  AGTGGGAGCTAGGCGTGAAACCCAGCGACCAGCCCCTGCTTGCAACGGAGCCCTCCCTGAACCCCAGGGA
  GAACCGTGAGAAGATGGCAGAAGTCATGTTCGAGAACTTCGGCGTGCCCGCTTTCTACCTGTCGGACCAG
  GCGGTGCTGGCTCTCTACGCCTCGGCCTGTGTCACGGGCCTGGTGGTGGACAGCGGGGATGCGGTCACCT
  GCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCCCACGCAGTCACCAAGCTCCACGTGGCGGGCAGGGA
  CATCACGGAGCTCCTCATGCAGCTGCTCCTGGCCAGCGGCCACACCTTCCCCTGCCAGCTGGACAAGGGT
  CTCGTGGACGACATCAAAAAGAAGCTGTGCTACGTGGCCTTGGAGCCCGAGAAGGAGCTTTCCCGGAGGC
  CGGAGGAGGTCCTGAGGGAGTACAAGCTGCCCGACGGGAACATCATCAGCCTCGGGGACCCGCTGCACCA
  GGCGCCCGAGGCCCTGTTCGTGCCCCAGCAGCTGGGCAGCCAGAGCCCCGGGCTCTCGAATATGGTCTCC
  AGCAGCATCACCAAGTGTGATACCGACATCCAGAAGATCCTCTTTGGGGAGATTGTGCTGTCGGGGGGCA
  CTACCCTGTTCCACGGGCTGGATGACCGGCTTCTCAAGGAGCTGGAGCAGCTGGCCTCCAAGGACACCCC
  CATCAAGATCACGGCTCCCCCCGACCGGTGGTTCTCCACCTGGATTGGAGCCTCCATCGTCACCTCTCTG
  AGTAGCTTCAAGCAGATGTGGGTCACCGCCGCAGACTTCAAGGAGTTTGGGACCTCCGTGGTGCAGAGAA
  GATGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206098 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFNPHALDSPAVIFDNGSGFCKAGLSGEFGPRHMVSSIVGHLKFQAPSAEANQKYFVGEEALYKQEALQL
  HSPFERGLITGWDDVERLWKHLFEWELGVKPSDQPLLATEPSLNPRENREKMAEVMFENFGVPAFYLSDQ
  AVLALYASACVTGLVVDSGDAVTCTVPIFEGYSLPHAVTKLHVAGRDITELLMQLLLASGHTFPCQLDKG
  LVDDIKKKLCYVALEPEKELSRRPEEVLREYKLPDGNIISLGDPLHQAPEALFVPQQLGSQSPGLSNMVS
  SSITKCDTDIQKILFGEIVLSGGTTLFHGLDDRLLKELEQLASKDTPIKITAPPDRWFSTWIGASIVTSL
  SSFKQMWVTAADFKEFGTSVVQRRCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080431
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080431.5
• RefSeq Size: 1437 bp
• RefSeq ORF: 1134 bp
• Locus ID: 140625
• UniProt ID: Q8TDY3
• Cytogenetics: 1p36.32
• MW: 41.6 kDa
• Gene Summary: The protein encoded by this intronless gene belongs to the actin family. Studies have shown that this protein may be involved in cytoskeletal organization similar to other cytoplasmic actin-related protein (ARP) subfamily members. Antibody raised against the human protein has been used to detect the protein by immunoblotting and immunofluorescence microscopy, demonstrating its specific synthesis in the testis, late in spermatid differentiation, and its localization in the calyx. [provided by RefSeq, Jul 2008]