PIP4P2 (NM_018710) Human Tagged ORF Clone

CAT#: RC206084

TMEM55A (Myc-DDK-tagged)-Human transmembrane protein 55A (TMEM55A)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_018710" in other vectors (6)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


TMEM55A Rabbit polyclonal Antibody
    • 100 ul

USD 365.00

Other products for "PIP4P2"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PIP4P2
Synonyms TMEM55A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206084 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGATGGGGTGGACGAACGCTCGCCTCTGCTGTCAGCATCCCACTCCGGAAATGTCACTCCCA
CCGCCCCACCGTACTTGCAAGAAAGCAGCCCCAGAGCGGAGCTCCCACCTCCATATACAGCCATTGCCAG
TCCAGACGCCAGTGGTATTCCAGTAATAAACTGCCGTGTGTGCCAATCACTAATCAATTTGGATGGCAAG
CTTCACCAGCATGTGGTTAAGTGCACAGTTTGCAATGAAGCTACGCCAATCAAAAACCCCCCAACAGGCA
AGAAATATGTTAGATGCCCTTGTAATTGTCTTCTCATTTGTAAGGACACATCTCGGCGAATAGGATGCCC
AAGACCCAACTGTAGACGGATAATTAACCTTGGCCCAGTAATGCTTATTTCTGAAGAACAACCAGCTCAG
CCTGCATTGCCAATCCAACCAGAAGGTACAAGGGTCGTGTGTGGGCACTGTGGAAACACATTCCTGTGGA
TGGAACTGAGGTTCAACACTCTGGCAAAATGCCCACACTGCAAAAAAATCTCCTCAGTGGGTAGTGCACT
TCCACGAAGACGCTGCTGTGCATATATTACCATTGGAATGATATGTATTTTCATTGGAGTTGGGTTAACT
GTTGGCACCCCAGATTTTGCAAGGCGATTTCGAGCAACCTATGTTTCTTGGGCAATTGCTTATCTCCTAG
GATTGATCTGCCTTATCCGAGCTTGTTATTGGGGAGCCATAAGAGTCAGTTATCCAGAACACAGTTTTGC
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206084 protein sequence
Red=Cloning site Green=Tags(s)

MAADGVDERSPLLSASHSGNVTPTAPPYLQESSPRAELPPPYTAIASPDASGIPVINCRVCQSLINLDGK
LHQHVVKCTVCNEATPIKNPPTGKKYVRCPCNCLLICKDTSRRIGCPRPNCRRIINLGPVMLISEEQPAQ
PALPIQPEGTRVVCGHCGNTFLWMELRFNTLAKCPHCKKISSVGSALPRRRCCAYITIGMICIFIGVGLT
VGTPDFARRFRATYVSWAIAYLLGLICLIRACYWGAIRVSYPEHSFA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018710
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018710.3
RefSeq Size 2420 bp
RefSeq ORF 774 bp
Locus ID 55529
UniProt ID Q8N4L2
Cytogenetics 8q21.3
Protein Families Transmembrane
MW 28.1 kDa
Gene Summary TMEM55A catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate (Ungewickell et al., 2005 [PubMed 16365287]).[supplied by OMIM, Mar 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.