PIP4P2 (NM_018710) Human Tagged ORF Clone

CAT#: RC206084

TMEM55A (Myc-DDK-tagged)-Human transmembrane protein 55A (TMEM55A)

Product Images

Circular map for RC206084

• 

  Western blot validation of overexpression lysate (Cat# LY402708) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206084 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIP4P2
• Synonyms: TMEM55A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206084 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGATGGGGTGGACGAACGCTCGCCTCTGCTGTCAGCATCCCACTCCGGAAATGTCACTCCCA
  CCGCCCCACCGTACTTGCAAGAAAGCAGCCCCAGAGCGGAGCTCCCACCTCCATATACAGCCATTGCCAG
  TCCAGACGCCAGTGGTATTCCAGTAATAAACTGCCGTGTGTGCCAATCACTAATCAATTTGGATGGCAAG
  CTTCACCAGCATGTGGTTAAGTGCACAGTTTGCAATGAAGCTACGCCAATCAAAAACCCCCCAACAGGCA
  AGAAATATGTTAGATGCCCTTGTAATTGTCTTCTCATTTGTAAGGACACATCTCGGCGAATAGGATGCCC
  AAGACCCAACTGTAGACGGATAATTAACCTTGGCCCAGTAATGCTTATTTCTGAAGAACAACCAGCTCAG
  CCTGCATTGCCAATCCAACCAGAAGGTACAAGGGTCGTGTGTGGGCACTGTGGAAACACATTCCTGTGGA
  TGGAACTGAGGTTCAACACTCTGGCAAAATGCCCACACTGCAAAAAAATCTCCTCAGTGGGTAGTGCACT
  TCCACGAAGACGCTGCTGTGCATATATTACCATTGGAATGATATGTATTTTCATTGGAGTTGGGTTAACT
  GTTGGCACCCCAGATTTTGCAAGGCGATTTCGAGCAACCTATGTTTCTTGGGCAATTGCTTATCTCCTAG
  GATTGATCTGCCTTATCCGAGCTTGTTATTGGGGAGCCATAAGAGTCAGTTATCCAGAACACAGTTTTGC
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206084 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAADGVDERSPLLSASHSGNVTPTAPPYLQESSPRAELPPPYTAIASPDASGIPVINCRVCQSLINLDGK
  LHQHVVKCTVCNEATPIKNPPTGKKYVRCPCNCLLICKDTSRRIGCPRPNCRRIINLGPVMLISEEQPAQ
  PALPIQPEGTRVVCGHCGNTFLWMELRFNTLAKCPHCKKISSVGSALPRRRCCAYITIGMICIFIGVGLT
  VGTPDFARRFRATYVSWAIAYLLGLICLIRACYWGAIRVSYPEHSFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018710
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018710.3
• RefSeq Size: 2420 bp
• RefSeq ORF: 774 bp
• Locus ID: 55529
• UniProt ID: Q8N4L2
• Cytogenetics: 8q21.3
• Protein Families: Transmembrane
• MW: 28.1 kDa
• Gene Summary: TMEM55A catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate (Ungewickell et al., 2005 [PubMed 16365287]).[supplied by OMIM, Mar 2008]