DR1 (NM_001938) Human Tagged ORF Clone

CAT#: RC205733

DR1 (Myc-DDK-tagged)-Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1)

Product Images

Circular map for RC205733

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  Western blot validation of overexpression lysate (Cat# LY419640) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205733 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DR1 protein (Cat# TP305733). The protein was produced from HEK293T cells transfected with DR1 cDNA clone (Cat# RC205733) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DR1
• Synonyms: NC2; NC2-BETA; NC2B; NCB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205733 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCTCGTCTGGCAACGATGATGATCTCACTATCCCCAGAGCTGCTATCAATAAAATGATCAAAG
  AGACTCTTCCTAATGTCCGGGTGGCCAACGATGCTCGAGAGCTGGTGGTGAACTGCTGCACTGAATTCAT
  TCACCTTATATCTTCTGAAGCCAATGAGATTTGTAACAAATCGGAAAAGAAGACCATCTCACCAGAGCAT
  GTCATACAAGCACTAGAAAGTTTGGGATTTGGCTCTTACATCAGTGAAGTAAAAGAAGTCTTGCAAGAGT
  GTAAAACAGTAGCATTAAAAAGAAGAAAGGCCAGTTCTCGTTTGGAAAACCTTGGCATTCCTGAAGAAGA
  GTTATTGAGACAGCAACAAGAATTATTTGCAAAAGCTAGACAGCAACAAGCAGAATTGGCCCAACAGGAA
  TGGCTTCAAATGCAGCAAGCTGCCCAACAAGCCCAGCTTGCTGCTGCCTCAGCCAGTGCATCTAATCAGG
  CGGGATCTTCTCAGGATGAAGAAGATGATGATGATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205733 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEH
  VIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQE
  WLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001938
• ORF Size: 528 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001938.3
• RefSeq Size: 3222 bp
• RefSeq ORF: 531 bp
• Locus ID: 1810
• UniProt ID: Q01658
• Cytogenetics: 1p22.1
• Domains: CBFD_NFYB_HMF
• Protein Families: Transcription Factors
• MW: 19.4 kDa
• Gene Summary: This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq, Jul 2008]