WDR91 (NM_014149) Human Tagged ORF Clone

CAT#: RC205719

WDR91 (Myc-DDK-tagged)-Human WD repeat domain 91 (WDR91)

Product Images

Circular map for RC205719

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  Western blot validation of overexpression lysate (Cat# LY415476) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205719 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR91 protein (Cat# TP305719). The protein was produced from HEK293T cells transfected with WDR91 cDNA clone (Cat# RC205719) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR91
• Synonyms: HSPC049; SORF-1; SORF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205719 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCCGTGGAGCGCACTGACGAGCTGGTCCGGGAGTACCTGCTCTTCCGCGGGTTCACGCACA
  CACTGCGGCAGCTGGACGCCGAGATCAAGGCGGACAAGGAGAAGGGGTTCCGGGTGGATAAGATTGTGGA
  CCAGCTGCAGCAGTTAATGCAGGTGTATGACTTGGCTGCCCTTCGGGATTATTGGAGCTACTTGGAGCGT
  CGGCTCTTCAGCCGCTTGGAGGATATATACAGACCCACAATCCACAAGCTGAAAACCAGCCTGTTTCGAT
  TTTATCTTGTCTACACAATCCAGACAAACAGAAATGACAAGGCTCAGGAGTTCTTTGCAAAGCAGGCCAC
  GGAACTCCAGAACCAGGCTGAGTGGAAGGATTGGTTTGTCCTGCCCTTCCTGCCATCCCCGGACACCAAC
  CCCACCTTTGCTACCTACTTTTCTCGACAGTGGGCTGACACCTTCATTGTGTCCCTGCACAACTTCCTGA
  GCGTCCTGTTTCAGTGCATGCCAGTCCCTGTGATCCTGAACTTTGATGCGGAGTGTCAGAGGACTAACCA
  GGTTCAAGAAGAAAATGAAGTTCTGCGTCAGAAGCTTTTTGCATTGCAAGCTGAAATCCACCGACTGAAG
  AAAGAGGAGCAACAGCCAGAAGAGGAAGAGGCCTTGGTCCAACACAAATTGCCTCCTTATGTCTCCAACA
  TGGACCGCCTGGGGGACTCGGAACTTGCCATGGTGTGCAGCCAAAGGAATGCCTCCCTCTCCCAGTCACC
  TCGTGTGGGCTTCCTGTCCTCGCTGCTGCCTCAGAGTAAGAAGAGCCCCTCAAGGTTGTCGCCTGCTCAG
  GGCCCTCCTCAACCTCAGAGCTCGGCCAAGAAAGAGTCCTTCGGTGGTCAGGGCACCAAGGGAAAGGACC
  CGACGTCCGGAGCCAAGGATGGGAAGAGCCTCCTCAGCGGGCTGGCCACTGGGGAGTCCGGTTGGTCACA
  GCACCGGCAGCGGCGCCTGCAGGACCATGGCAAGGAGAGGAAGGAGCTTTTCTCCACAACCACTTCCCAG
  TGTGCAGAGAAGAAACCAGAAGCCAGTGGCCCAGAGGCTGAGCCCTGCCCAGAGCTCCACACGGAGCCAG
  TGGAGCCACTGACTCGGGCATCCTCGGCAGGCCCTGAGGGTGGAGGAGTCCGCCCCGAGCAGCCCTTTAT
  TGTGCTGGGACAGGAGGAGTACGGGGAACACCACTCATCCATCATGCACTGCAGAGTGGACTGCTCTGGG
  AGGAGAGTCGCCAGCTTAGACGTAGATGGGGTCATCAAAGTGTGGTCCTTCAACCCCATCATGCAGACCA
  AAGCATCCTCCATTTCCAAATCACCGCTGCTGTCTTTGGAATGGGCCACCAAACGGGACAGACTGCTCTT
  GCTGGGCAGTGGTGTGGGAACAGTGCGTCTCTATGACACGGAAGCCAAGAAGAATCTCTGTGAAATCAAT
  ATCAACGACAACATGCCCAGAATCCTGTCTCTTGCGTGCAGCCCCAACGGGGCCTCTTTCGTCTGTTCGG
  CAGCAGCTCCGAGCCTCACTTCCCAGGTGGACTTCTCAGCACCAGACATCGGCAGCAAGGGCATGAACCA
  GGTTCCTGGCAGGCTGCTGCTGTGGGACACGAAAACCATGAAGCAGCAGCTCCAGTTCTCCCTGGATCCA
  GAACCCATTGCTATCAACTGTACAGCCTTCAATCACAACGGGAACCTGCTGGTCACAGGGGCAGCTGATG
  GCGTCATCCGGCTGTTTGACATGCAGCAGCATGAGTGCGCGATGAGCTGGAGGGCCCACTACGGGGAGGT
  CTACTCTGTGGAGTTCAGCTATGATGAGAACACCGTGTACAGCATCGGCGAGGACGGGAAGTTCATCCAG
  TGGAACATCCACAAGAGTGGCCTCAAGGTATCCGAGTACAGCCTCCCCTCAGATGCCACGGGCCCCTTTG
  TGCTGTCTGGATACAGCGGCTACAAGCAGGTTCAAGTCCCCAGGGGCCGACTCTTCGCTTTTGACTCGGA
  GGGAAATTACATGCTGACATGTTCTGCCACAGGCGGCGTCATCTACAAGCTGGGTGGCGATGAGAAGGTT
  CTGGAGAGCTGCTTGAGCCTAGGTGGCCACCGAGCCCCTGTGGTCACCGTGGACTGGAGCACTGCCATGG
  ACTGTGGGACCTGCCTCACCGCCTCCATGGATGGCAAGATCAAGCTGACCACCCTCCTGGCCCATAAAGC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205719 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAVERTDELVREYLLFRGFTHTLRQLDAEIKADKEKGFRVDKIVDQLQQLMQVYDLAALRDYWSYLER
  RLFSRLEDIYRPTIHKLKTSLFRFYLVYTIQTNRNDKAQEFFAKQATELQNQAEWKDWFVLPFLPSPDTN
  PTFATYFSRQWADTFIVSLHNFLSVLFQCMPVPVILNFDAECQRTNQVQEENEVLRQKLFALQAEIHRLK
  KEEQQPEEEEALVQHKLPPYVSNMDRLGDSELAMVCSQRNASLSQSPRVGFLSSLLPQSKKSPSRLSPAQ
  GPPQPQSSAKKESFGGQGTKGKDPTSGAKDGKSLLSGLATGESGWSQHRQRRLQDHGKERKELFSTTTSQ
  CAEKKPEASGPEAEPCPELHTEPVEPLTRASSAGPEGGGVRPEQPFIVLGQEEYGEHHSSIMHCRVDCSG
  RRVASLDVDGVIKVWSFNPIMQTKASSISKSPLLSLEWATKRDRLLLLGSGVGTVRLYDTEAKKNLCEIN
  INDNMPRILSLACSPNGASFVCSAAAPSLTSQVDFSAPDIGSKGMNQVPGRLLLWDTKTMKQQLQFSLDP
  EPIAINCTAFNHNGNLLVTGAADGVIRLFDMQQHECAMSWRAHYGEVYSVEFSYDENTVYSIGEDGKFIQ
  WNIHKSGLKVSEYSLPSDATGPFVLSGYSGYKQVQVPRGRLFAFDSEGNYMLTCSATGGVIYKLGGDEKV
  LESCLSLGGHRAPVVTVDWSTAMDCGTCLTASMDGKIKLTTLLAHKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014149
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014149.4
• RefSeq Size: 4619 bp
• RefSeq ORF: 2244 bp
• Locus ID: 29062
• UniProt ID: A4D1P6
• Cytogenetics: 7q33
• Domains: WD40
• MW: 83.3 kDa
• Gene Summary: Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport (PubMed:26783301). It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome (PubMed:27126989). May play a role in meiosis (By similarity).[UniProtKB/Swiss-Prot Function]