ZNRF4 (NM_181710) Human Tagged ORF Clone

CAT#: RC205571

ZNRF4 (Myc-DDK-tagged)-Human zinc and ring finger 4 (ZNRF4)

Product Images

Circular map for RC205571

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNRF4
• Synonyms: RNF204; SPERIZIN; spzn; Ssrzf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205571 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTCTGCCGTCCGGAGCACTTAATGCCTAGAGCCAGCAGGGTCCCAGTGGCCGCGTCACTGCCTC
  TGAGCCACGCGGTCATTCCAACTCAACTGCCCTCGCGTTCTGGCCACAGGCCCCCTGGGAGACCCCGGAG
  ATGCCCAAAGGCCTCATGCCTGCCGCCTCCAGTGGGACCTAGCAGCACACAGACAGCGAAGCGGGTGACC
  ATGGGGTGGCCACGGCCGGGCCAAGCCCTCGTGGCAGTCAAAGCCTTGCTGGTCTTGTCGCTGCTCCAGG
  TGCCCGCGCAGGCGGTGGTACGGGCCGTGCTGGAAGACAACTCGAGCTCGGTGGACTTTGCGGATCTGCC
  GGCGCTGTTCGGCGTCCCCCTGGCCCCCGAGGGCATACGGGGCTACCTGATGGAGGTCAAGCCAGCCAAC
  GCGTGCCATCCCATCGAGGCCCCGCGACTGGGCAACCGCTCTCTGGGCTCCATCGCGCTGATCCGCCACT
  ACGACTGCACCTTCGACCTCAAGGTGCTGAACGCCCAGCGCGCCGGCTTCGAGGCGGCCATCGTGCACAA
  CGTCCACTCCGACGACCTCGTGAGCATGACCCACGTCTACGAGGACTTGAGGGGCCAGATCGCCATCCCC
  TCAGTGTTCGTGAGCGAGGCCGCCTCGCAGGACCTGCGGGTCATCCTGGGCTGCAACAAGTCGGCCCACG
  CGCTGCTCCTGCCCGACGACCCACCGTGCCACGACCTGGGCTGTCACCCCGTGCTGACCGTGTCCTGGGT
  GCTGGGCTGTACCCTGGCCCTGGTCGTATCAGCCTTCTTTGTCCTGAACCACCTGTGGCTCTGGGCCCAG
  GCCTGCTGCAGCCACAGACGGCCGGTGAAGACGTCTACCTGCCAGAAGGCCCAGGTCCGCACCTTCACGT
  GGCACAACGACCTGTGTGCCATCTGCCTGGATGAGTACGAGGAGGGCGACCAACTCAAGATCCTGCCCTG
  CTCCCACACCTACCACTGCAAATGCATTGACCCCTGGTTCTCCCAAGCCCCCCGGCGCTCCTGCCCCGTG
  TGCAAACAGTCGGTGGCCGCCACAGAAGACAGCTTTGACTCCACCACCTACAGCTTCAGGGACGAGGACC
  CCTCCCTACCGGGCCACCGGCCCCCCATCTGGGCCATTCAAGTCCAGCTACGCTCCCGGAGGCTGGAGCT
  GCTGGGCCGCGCCAGTCCCCACTGCCACTGCAGCACCACGTCCCTGGAGGCAGAGTATACCACTGTCTCC
  TCAGCCCCTCCTGAGGCCCCTGGTCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205571 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLCRPEHLMPRASRVPVAASLPLSHAVIPTQLPSRSGHRPPGRPRRCPKASCLPPPVGPSSTQTAKRVT
  MGWPRPGQALVAVKALLVLSLLQVPAQAVVRAVLEDNSSSVDFADLPALFGVPLAPEGIRGYLMEVKPAN
  ACHPIEAPRLGNRSLGSIALIRHYDCTFDLKVLNAQRAGFEAAIVHNVHSDDLVSMTHVYEDLRGQIAIP
  SVFVSEAASQDLRVILGCNKSAHALLLPDDPPCHDLGCHPVLTVSWVLGCTLALVVSAFFVLNHLWLWAQ
  ACCSHRRPVKTSTCQKAQVRTFTWHNDLCAICLDEYEEGDQLKILPCSHTYHCKCIDPWFSQAPRRSCPV
  CKQSVAATEDSFDSTTYSFRDEDPSLPGHRPPIWAIQVQLRSRRLELLGRASPHCHCSTTSLEAEYTTVS
  SAPPEAPGQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181710
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181710.2, NP_859061.2
• RefSeq Size: 1442 bp
• RefSeq ORF: 1290 bp
• Locus ID: 148066
• UniProt ID: Q8WWF5
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 46.9 kDa
• Gene Summary: E3 ubiquitin-protein ligase which specifically induces ubiquitination and proteasomal degradation of CANX within the endoplasmic reticulum (PubMed:21205830). Could have a role in spermatogenesis (By similarity).[UniProtKB/Swiss-Prot Function]