C6orf57 (SDHAF4) (NM_145267) Human Tagged ORF Clone

CAT#: RC205542

C6orf57 (Myc-DDK-tagged)-Human chromosome 6 open reading frame 57 (C6orf57)

Product Images

Circular map for RC205542

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  Western blot validation of overexpression lysate (Cat# LY407992) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205542 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SDHAF4 protein (Cat# TP305542). The protein was produced from HEK293T cells transfected with SDHAF4 cDNA clone (Cat# RC205542) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C6orf57
• Synonyms: C6orf57; Sdh8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205542 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCATCGAGGCTTCCCTGGTTGCTTAGCTGGGTCTCGGCCACGGCGTGGAGAGCGGCAAGATCAC
  CCCTTCTGTGTCATTCTCTGAGGAAAACAAGTTCTTCTCAAGGAGGAAAGTCTGAACTTGTCAAACAGTC
  CCTTAAGAAGCCGATGTTACCAGAAGGTCGTTTTGATGCACCAGAGGATTCCCATTTAGAGAAAGAACCA
  CTGGAAAAATTTCCAGATGATGTTAATCCAGTGACCAAAGAAAAAGGTGGACCCAGGGGCCCAGAACCTA
  CCCGATATGGAGATTGGGAACGAAAAGGACGCTGTATTGATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205542 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPSRLPWLLSWVSATAWRAARSPLLCHSLRKTSSSQGGKSELVKQSLKKPMLPEGRFDAPEDSHLEKEP
  LEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCIDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145267
• ORF Size: 324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145267.1
• RefSeq Size: 526 bp
• RefSeq ORF: 327 bp
• Locus ID: 135154
• UniProt ID: Q5VUM1
• Cytogenetics: 6q13
• Protein Families: Secreted Protein
• MW: 12.2 kDa
• Gene Summary: Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol (PubMed:24954416). Binds to the flavoprotein subunit SDHA in its FAD-bound form, blocking the generation of excess reactive oxigen species (ROS) and facilitating its assembly with the iron-sulfur protein subunit SDHB into the SDH catalytic dimer (By similarity).[UniProtKB/Swiss-Prot Function]