TSGA10 (NM_025244) Human Tagged ORF Clone
CAT#: RC205528
TSGA10 (Myc-DDK-tagged)-Human testis specific, 10 (TSGA10), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_025244" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TSGA10 |
Synonyms | CEP4L; CT79; SPGF26 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205528 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCGAAGTAGGTCTAAAAGTCCAAGACGCCCATCACCAACTGCCCGGGGTGCAAACTGTGATGTAG AACTTTTGAAGACAACAACAAGAGATCGTGAAGAACTTAAATGCATGCTGGAAAAATATGAGCGCCATTT GGCAGAAATTCAGGGTAATGTCAAGGTTCTTAAATCTGAGAGAGACAAGATCTTCCTTCTTTATGAACAG GCACAGGAAGAAATTACCCGACTTCGACGAGAAATGATGAAAAGCTGTAAGAGTCCTAAATCAACAACGG CACATGCTATTCTCCGGCGAGTGGAGACTGAAAGAGATGTAGCCTTTACTGATTTACGAAGAATGACCAC AGAACGAGATAGTCTAAGGGAGAGGCTAAAGATTGCTCAAGAGACAGCATTTAATGAGAAGGCTCACCTG GAACAAAGGATAGAGGAGCTGGAGTGTACAGTTCATAATCTTGATGATGAACGTATGGAGCAAATGTCAA ATATGACTTTGATGAAGGAAACCATAAGCACTGTGGAAAAAGAAATGAAATCACTAGCAAGAAAGGCAAT GGATACCGAAAGTGAACTTGGCAGACAAAAAGCAGAGAATAATTCTTTGAGACTTTTGTATGAAAACACA GAAAAAGATCTTTCTGATACTCAGCGACACCTTGCTAAGAAAAAATATGAGCTACAGCTTACTCAGGAGA AAATTATGTGCTTGGATGAAAAAATTGATAACTTTACAAGGCAAAATATTGCACAGCGAGAAGAAATCAG CATTCTTGGTGGAACCCTCAATGATCTGGCTAAAGAAAAGGAATGCATGCAAGCATGTTTGGATAAAAAA TCTGAGAATATTGCATCCCTTGGAGAGAGTTTGGCAATGAAAGAAAAGACCATTTCAGGCATGAAGAATA TCATTGCTGAGATGGAACAGGCATCAAGACAGTGTACTGAGGCCCTAATTGTGTGTGAACAAGACGTTTC CAGAATGCGTCGGCAATTGGATGAGACAAATGATGAGCTGGCCCAGATCGCCAGGGAAAGAGATATCTTG GCTCATGACAATGACAATCTCCAGGAACAGTTTGCTAAAGCTAAACAAGAAAACCAGGCACTGTCCAAAA AATTGAATGACACTCATAATGAACTTAATGACATAAAACAGAAGGTTCAAGATACTAATTTGGAGGTTAA CAAGCTGAAGAATATATTAAAGTCTGAAGAATCTGAGAACCGGCAAATGATGGAACAACTTCGAAAAGCC AATGAAGATGCTGAAAACTGGGAAAATAAAGCCCGTCAATCAGAGGCAGATAACAATACCCTCAAACTGG AACTTATCACTGCTGAGGCAGAGGGTAACAGATTAAAAGAAAAAGTAGATTCCCTCAACAGAGAGGTTGA GCAACACTTAAATGCAGAAAGGTCTTACAAGTCCCAGATTTCTACCTTACATAAATCTGTTGTAAAAATG GAAGAGGAGCTTCAGAAGGTTCAGTTTGAAAAAGTGTCCGCTCTTGCAGATTTGTCTTCTACTAGGGAAC TCTGTATTAAACTTGACTCAAGCAAAGAACTTCTTAATCGACAGCTGGTTGCTAAAGATCAAGAAATAGA AATGAGGGAGAATGAGTTAGATTCTGCTCATTCTGAAATTGAACTCCTGAGGAGTCAGATGGCAAATGAG AGAATCTCCATGCAGAATCTAGAAGCTTTGCTGGTGGCCAATCGAGACAAAGAATATCAGTCTCAGATAG CACTTCAAGAAAAAGAATCTGAAATTCAGCTTCTTAAAGAACACCTTTGTTTGGCAGAAAATAAAATGGC CATCCAGAGTAGAGATGTGGCCCAGTTCAGAAATGTTGTCACACAATTGGAAGCTGATTTAGACATTACC AAAAGACAACTAGGAACAGAGCGCTTTGAAAGGGAGAGGGCCGTACAAGAACTTCGCCGCCAAAATTATT CAAGTAATGCTTATCATATGAGTTCTACAATGAAGCCAAATACAAAATGTCATTCACCAGAACGTGCTCA CCATCGATCTCCTGACCGAGGCCTAGATCGATCATTAGAAGAGAATCTTTGCTACAGAGATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205528 protein sequence
Red=Cloning site Green=Tags(s) MMRSRSKSPRRPSPTARGANCDVELLKTTTRDREELKCMLEKYERHLAEIQGNVKVLKSERDKIFLLYEQ AQEEITRLRREMMKSCKSPKSTTAHAILRRVETERDVAFTDLRRMTTERDSLRERLKIAQETAFNEKAHL EQRIEELECTVHNLDDERMEQMSNMTLMKETISTVEKEMKSLARKAMDTESELGRQKAENNSLRLLYENT EKDLSDTQRHLAKKKYELQLTQEKIMCLDEKIDNFTRQNIAQREEISILGGTLNDLAKEKECMQACLDKK SENIASLGESLAMKEKTISGMKNIIAEMEQASRQCTEALIVCEQDVSRMRRQLDETNDELAQIARERDIL AHDNDNLQEQFAKAKQENQALSKKLNDTHNELNDIKQKVQDTNLEVNKLKNILKSEESENRQMMEQLRKA NEDAENWENKARQSEADNNTLKLELITAEAEGNRLKEKVDSLNREVEQHLNAERSYKSQISTLHKSVVKM EEELQKVQFEKVSALADLSSTRELCIKLDSSKELLNRQLVAKDQEIEMRENELDSAHSEIELLRSQMANE RISMQNLEALLVANRDKEYQSQIALQEKESEIQLLKEHLCLAENKMAIQSRDVAQFRNVVTQLEADLDIT KRQLGTERFERERAVQELRRQNYSSNAYHMSSTMKPNTKCHSPERAHHRSPDRGLDRSLEENLCYRDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025244 |
ORF Size | 2094 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025244.4 |
RefSeq Size | 3685 bp |
RefSeq ORF | 2097 bp |
Locus ID | 80705 |
UniProt ID | Q9BZW7 |
Cytogenetics | 2q11.2 |
MW | 81.4 kDa |
Gene Summary | Plays a role in spermatogenesis (PubMed:28905369). When overexpressed, prevents nuclear localization of HIF1A (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205528L3 | Lenti ORF clone of Human testis specific, 10 (TSGA10), transcript variant 1, Myc-DDK-tagged |
USD 939.00 |
|
RC205528L4 | Lenti ORF clone of Human testis specific, 10 (TSGA10), transcript variant 1, mGFP tagged |
USD 939.00 |
|
RG205528 | TSGA10 (tGFP-tagged) - Human testis specific, 10 (TSGA10), transcript variant 1 |
USD 839.00 |
|
SC305250 | TSGA10 (untagged)-Human testis specific, 10 (TSGA10), transcript variant 1 |
USD 640.00 |
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