Cleavage stimulation factor 2 (CSTF2) (NM_001325) Human Tagged ORF Clone
CAT#: RC204450
CSTF2 (Myc-DDK-tagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_001325" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cleavage stimulation factor 2 |
Synonyms | CstF-64 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204450 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTGGGGAACATTC CTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGACCTGTTGTTAGTTTCAGATT GGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGCTTCTGTGAATACCAAGACCAAGAGACAGCA CTTAGTGCCATGCGGAACCTGAATGGGCGCGAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCA GTGAAAAGAACAAAGAAGAGCTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGA GACCATCAGTCCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAACATGTTACTTC AGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGAATTGTGGATCCGGAAATTGC CCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTGATTGCAGGCAACCCTCAGCCAGTCCATGGT GCTGGGCCTGGCTCAGGATCCAATGTGTCAATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTT TGGGTGGAATGCATGTCAATGGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACC AGGGCAAATGCCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAAGACCCCAGAG CAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGCTTGTTAGGAGATGCTCCGAA TGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAGGTAGAGCCTAGAGGTTACTTGGGACCACCT CATCAGGGTCCACCCATGCACCATGTCCCTGGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGG GAGGGCCATTACCCGAGCCCAGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCC ACCCTTGGATGGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATGGAAGCTCGTG CAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATGGAGGCCAGAGGCATGGATAC CAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGTGGAATGCAGGGTCCCAGTCCAATTAACATG GGGGCGGTTGTCCCCCAGGGATCCAGACAGGTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTA TACAGGGTGGAAGCCAGCCTGGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAA GGCTGCTTTGATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204450 protein sequence
Red=Cloning site Green=Tags(s) MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETA LSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQM FELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHG AGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQA QVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPP HQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAM EARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINM GAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQR QSILILKEQIQKSTGAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001325 |
ORF Size | 1731 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001325.3 |
RefSeq Size | 2027 bp |
RefSeq ORF | 1734 bp |
Locus ID | 1478 |
UniProt ID | P33240 |
Cytogenetics | Xq22.1 |
Domains | RRM |
MW | 61 kDa |
Gene Summary | This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204450L1 | Lenti ORF clone of Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), Myc-DDK-tagged |
USD 837.00 |
|
RC204450L2 | Lenti ORF clone of Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), mGFP tagged |
USD 837.00 |
|
RC204450L3 | Lenti ORF clone of Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), Myc-DDK-tagged |
USD 837.00 |
|
RC204450L4 | Lenti ORF clone of Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), mGFP tagged |
USD 837.00 |
|
RG204450 | CSTF2 (tGFP-tagged) - Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2) |
USD 737.00 |
|
SC119306 | CSTF2 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2) |
USD 538.00 |
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