NKIRAS1 (NM_020345) Human Tagged ORF Clone
CAT#: RC204248
NKIRAS1 (Myc-DDK-tagged)-Human NFKB inhibitor interacting Ras-like 1 (NKIRAS1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_020345" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NKIRAS1 |
Synonyms | kappaB-Ras1; KBRAS1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204248 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGGGCTGCAAGGTTGTGGTTTGTGGATTGTTATCTGTGGGGAAAACTGCAATTTTGGAGCAGC TCCTTTATGGAAATCATACTATTGGAATGGAAGATTGCGAAACAATGGAAGATGTATACATGGCTTCAGT AGAAACAGACCGAGGAGTAAAAGAACAGTTACATCTTTATGACACCAGAGGTCTACAGGAAGGCGTGGAG CTGCCAAAGCATTATTTTTCATTTGCTGATGGCTTCGTTCTTGTGTACAGTGTGAATAACCTTGAATCCT TTCAAAGAGTGGAGCTTCTGAAGAAAGAAATCGATAAGTTCAAAGACAAAAAAGAGGTAGCAATTGTGGT ATTAGGAAACAAAATCGACCTTTCTGAGCAGAGACAAGTGGACGCTGAAGTGGCACAGCAGTGGGCAAAA AGTGAGAAAGTAAGACTGTGGGAGGTGACTGTTACAGATCGGAAAACTCTGATTGAACCATTCACTTTAT TAGCCAGTAAACTTTCTCAACCCCAGAGCAAATCAAGCTTTCCTTTGCCTGGGAGGAAAAACAAAGGGAA CTCTAATTCTGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204248 protein sequence
Red=Cloning site Green=Tags(s) MGKGCKVVVCGLLSVGKTAILEQLLYGNHTIGMEDCETMEDVYMASVETDRGVKEQLHLYDTRGLQEGVE LPKHYFSFADGFVLVYSVNNLESFQRVELLKKEIDKFKDKKEVAIVVLGNKIDLSEQRQVDAEVAQQWAK SEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLPGRKNKGNSNSEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020345 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020345.4 |
RefSeq Size | 2013 bp |
RefSeq ORF | 579 bp |
Locus ID | 28512 |
UniProt ID | Q9NYS0 |
Cytogenetics | 3p24.2 |
Domains | ras, RAS, RAB |
MW | 21.6 kDa |
Gene Summary | Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204248L3 | Lenti ORF clone of Human NFKB inhibitor interacting Ras-like 1 (NKIRAS1), Myc-DDK-tagged |
USD 600.00 |
|
RC204248L4 | Lenti ORF clone of Human NFKB inhibitor interacting Ras-like 1 (NKIRAS1), mGFP tagged |
USD 600.00 |
|
RG204248 | NKIRAS1 (tGFP-tagged) - Human NFKB inhibitor interacting Ras-like 1 (NKIRAS1) |
USD 500.00 |
|
SC108736 | NKIRAS1 (untagged)-Human NFKB inhibitor interacting Ras-like 1 (NKIRAS1) |
USD 300.00 |
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