CCDC90A (MCUR1) (NM_001031713) Human Tagged ORF Clone

CAT#: RC203684

CCDC90A (Myc-DDK-tagged)-Human coiled-coil domain containing 90A (CCDC90A)

Product Images

Circular map for RC203684

• 

  Western blot validation of overexpression lysate (Cat# LY422164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203684 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC90A
• Synonyms: C6orf79; CCDC90A; FMP32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203684 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGCGGCTCGGTCGGCGGCCAGAGGACCCAGCGCCTGCCCGGCCGCCAGCGGCTTCTCTTCTTGC
  CCGTGGGCCTCAGCGGAAGACCGGGCGGCAGCGAAACCTCAGCACGCCGCTGCCTCTCTGCGCTCTCCGA
  CGGTCTGGGGGCGCTGCGCCCTCGCGCCCCGGCGGCCCGCGGCGGCGTGTCACGTGCCTCACCGCTGCTC
  CTCCTCCTGCTAGTGCCCTCCCCGCGCTTGGCCGCCGCAGCCCCGCGCCGGCAGCTCGGGGACTGGGAGC
  GCTCGCGCCTCGGGTATGCCGCACCCCCGGCCGGGCGCAGCAGCGCGTGGAGGTGCTCACCGGGCGTCGC
  CGCTGCCGCCGGCGCCCTTCCCCAGTACCACGGCCCGGCGCCTGCACTCGTTTCCTGCAGGAGAGAGCTA
  AGCTTGTCTGCTGGATCCCTGCAGCTGGAGCGCAAAAGGAGAGATTTCACCTCTTCTGGGAGCAGGAAAC
  TCTACTTCGACACTCATGCCTTAGTGTGCTTACTGGAAGACAATGGGTTTGCTACTCAACAAGCAGAAAT
  CATTGTGTCTGCATTGGTCAAGATCCTGGAGGCCAACATGGACATCGTCTACAAAGATATGGTCACCAAG
  ATGCAGCAGGAAATCACTTTTCAGCAAGTAATGTCTCAGATTGCGAATGTGAAAAAGGATATGATTATTT
  TGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGAAAATGAGAAAATAAAACTCGAACTACATCAGTTAAA
  ACAACAAGTAATGGATGAAGTGATCAAAGTCCGAACAGATACCAAATTAGACTTCAACCTAGAAAAGAGC
  AGAGTAAAAGAATTGTATTCATTGAACGAAAAGAAGCTGCTGGAATTGAGAACAGAAATAGTGGCATTGC
  ATGCCCAGCAAGATCGGGCCCTTACCCAGACAGACAGGAAGATCGAAACTGAGGTTGCTGGCCTCAAAAC
  CATGCTTGAGTCACACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTA
  GCTCTGGGATTTTATCGCCTGTGGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203684 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDCGSVGGQRTQRLPGRQRLLFLPVGLSGRPGGSETSARRCLSALSDGLGALRPRAPAARGGVSRASPLL
  LLLLVPSPRLAAAAPRRQLGDWERSRLGYAAPPAGRSSAWRCSPGVAAAAGALPQYHGPAPALVSCRREL
  SLSAGSLQLERKRRDFTSSGSRKLYFDTHALVCLLEDNGFATQQAEIIVSALVKILEANMDIVYKDMVTK
  MQQEITFQQVMSQIANVKKDMIILEKSEFSALRAENEKIKLELHQLKQQVMDEVIKVRTDTKLDFNLEKS
  RVKELYSLNEKKLLELRTEIVALHAQQDRALTQTDRKIETEVAGLKTMLESHKLDNIKYLAGSIFTCLTV
  ALGFYRLWI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031713
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031713.3, NP_001026883.1
• RefSeq Size: 5472 bp
• RefSeq ORF: 1080 bp
• Locus ID: 63933
• UniProt ID: Q96AQ8
• Cytogenetics: 6p23
• Protein Families: Transmembrane
• MW: 39.7 kDa
• Gene Summary: Key regulator of mitochondrial calcium uniporter (MCU) required for calcium entry into mitochondrion (PubMed:23178883, PubMed:26445506, PubMed:27184846, PubMed:26976564). Plays a direct role in uniporter-mediated calcium uptake via a direct interaction with MCU (PubMed:23178883). Probably involved in the assembly of the membrane components of the uniporter complex (uniplex) (PubMed:27184846).[UniProtKB/Swiss-Prot Function]