MOSC1 (MARC1) (NM_022746) Human Tagged ORF Clone

CAT#: RC203674

MOSC1 (Myc-DDK-tagged)-Human MOCO sulphurase C-terminal domain containing 1 (MOSC1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC203674

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  Western blot validation of overexpression lysate (Cat# LY411585) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203674 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MARC1 protein (Cat# TP303674). The protein was produced from HEK293T cells transfected with MARC1 cDNA clone (Cat# RC203674) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOSC1
• Synonyms: MARC1; MOSC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203674 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCGCCGGCTCCTCCGCGCTGGCGCGCTTTGTCCTCCTCGCGCAATCCCGGCCCGGGTGGCTCG
  GGGTTGCCGCGCTGGGCCTGACCGCGGTGGCGCTGGGGGCTGTCGCCTGGCGCCGCGCATGGCCCACGCG
  GCGCCGGCGGCTGCTGCAGCAGGTGGGCACAGTGGCGCAGCTCTGGATCTACCCTGTGAAATCCTGCAAG
  GGGGTGCCGGTGAGCGAGGCGGAGTGCACGGCCATGGGGCTGCGCAGCGGCAACCTGCGGGACAGGTTTT
  GGCTTGTGATCAACCAGGAGGGAAACATGGTTACTGCTCGCCAGGAACCTCGCCTGGTCCTGATTTCCCT
  GACCTGCGATGGTGACACCCTGACTCTCAGTGCAGCCTACACAAAGGACCTACTACTGCCTATCAAAACG
  CCCACCACAAATGCAGTGCACAAGTGCAGAGTGCACGGCCTGGAGATAGAGGGCAGGGACTGTGGCGAGG
  CCGCCGCCCAGTGGATAACCAGCTTCCTGAAGTCACAGCCCTACCGCCTGGTGCACTTCGAGCCTCACAA
  GCGACCGAGACGTCCTCATCAAATAGCAGACTTGTTCCGACCCAAGGACCAGATTGCTTACTCAGACACC
  AGCCCATTCTTGATCCTTTCTGAGGCGTCGCTGGCGGATCTCAACTCCAGGCTAGAGAAGAAAGTTAAAG
  CAACCAACTTCAGGCCCAATATTGTAATTTCAGGATGCGATGTCTATGCAGAGGATTCTTGGGATGAGCT
  TCTTATTGGTGACGTGGAACTGAAAAGGGTGATGGCTTGTTCCAGATGCATTTTAACCACAGTGGACCCA
  GACACCGGTGTCATGAGCAGGAAGGAACCGCTGGAAACACTGAAGAGTTATCGCCAGTGTGACCCTTCAG
  AACGAAAGTTATATGGAAAATCACCACTCTTTGGGCAGTATTTTGTGCTGGAAAACCCAGGGACCATCAA
  AGTGGGAGACCCTGTGTACCTGCTGGGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203674 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAAGSSALARFVLLAQSRPGWLGVAALGLTAVALGAVAWRRAWPTRRRRLLQQVGTVAQLWIYPVKSCK
  GVPVSEAECTAMGLRSGNLRDRFWLVINQEGNMVTARQEPRLVLISLTCDGDTLTLSAAYTKDLLLPIKT
  PTTNAVHKCRVHGLEIEGRDCGEAAAQWITSFLKSQPYRLVHFEPHKRPRRPHQIADLFRPKDQIAYSDT
  SPFLILSEASLADLNSRLEKKVKATNFRPNIVISGCDVYAEDSWDELLIGDVELKRVMACSRCILTTVDP
  DTGVMSRKEPLETLKSYRQCDPSERKLYGKSPLFGQYFVLENPGTIKVGDPVYLLGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022746
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022746.2, NP_073583.2
• RefSeq Size: 2258 bp
• RefSeq ORF: 1014 bp
• Locus ID: 64757
• UniProt ID: Q5VT66
• Cytogenetics: 1q41
• Domains: MOSC_N
• Protein Families: Transmembrane
• MW: 37.5 kDa
• Gene Summary: As a component of an N-hydroxylated prodrug-converting complex required to reduce N-hydroxylated prodrugs, such as benzamidoxime. Also able to reduce N(omega)-hydroxy-L-arginine (NOHA) and N(omega)-hydroxy-N(delta)-methyl-L-arginine (NHAM) into L-arginine and N(delta)-methyl-L-arginine, respectively.[UniProtKB/Swiss-Prot Function]