CRSP9 (MED7) (NM_004270) Human Tagged Lenti ORF Clone
CAT#: RC202696L4
- LentiORF®
Lenti ORF clone of Human mediator complex subunit 7 (MED7), transcript variant 2, mGFP tagged
"NM_004270" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CRSP9 |
Synonyms | ARC34; CRSP9; CRSP33 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC202696).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004270 |
ORF Size | 699 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004270.3 |
RefSeq Size | 1066 bp |
RefSeq ORF | 702 bp |
Locus ID | 9443 |
UniProt ID | O43513 |
Cytogenetics | 5q33.3 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 27.1 kDa |
Gene Summary | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202696 | MED7 (Myc-DDK-tagged)-Human mediator complex subunit 7 (MED7), transcript variant 2 |
USD 300.00 |
|
RC202696L3 | Lenti ORF clone of Human mediator complex subunit 7 (MED7), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RG202696 | MED7 (tGFP-tagged) - Human mediator complex subunit 7 (MED7), transcript variant 2 |
USD 500.00 |
|
SC112668 | MED7 (untagged)-Human mediator complex subunit 7 (MED7), transcript variant 2 |
USD 300.00 |
|
SC320839 | MED7 (untagged)-Human mediator complex subunit 7 (MED7), transcript variant 2 |
USD 300.00 |
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