Mel18 (PCGF2) (NM_007144) Human Tagged ORF Clone

CAT#: RC202490

PCGF2 (Myc-DDK-tagged)-Human polycomb group ring finger 2 (PCGF2)

Product Images

Circular map for RC202490

• 

  Western blot validation of overexpression lysate (Cat# LY402096) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202490 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mel18
• Synonyms: MEL-18; RNF110; TPFS; ZNF144
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202490 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCGGACTACACGGATCAAAATCACAGAGCTGAACCCCCACCTCATGTGTGCCCTCTGCGGGGGGT
  ACTTCATCGACGCCACCACTATCGTGGAGTGCCTGCATTCCTTCTGCAAAACCTGCATCGTGCGCTACCT
  GGAGACCAACAAATACTGCCCCATGTGTGACGTGCAGGTCCATAAAACCCGGCCGCTGCTGAGCATCAGG
  TCTGACAAAACACTTCAAGACATTGTCTACAAATTGGTCCCTGGGCTTTTTAAAGATGAGATGAAACGGC
  GGCGGGATTTCTATGCAGCGTACCCCCTGACGGAGGTCCCCAACGGCTCCAATGAGGACCGCGGCGAGGT
  CTTGGAGCAGGAGAAGGGGGCTCTGAGTGATGATGAGATTGTCAGCCTCTCCATCGAATTCTACGAAGGT
  GCCAGGGACCGGGACGAGAAGAAGGGCCCCCTGGAGAATGGGGATGGGGACAAAGAGAAAACAGGGGTGC
  GCTTCCTGCGATGCCCAGCAGCCATGACCGTCATGCATCTTGCCAAGTTTCTCCGCAACAAGATGGATGT
  GCCCAGCAAGTACAAGGTGGAGGTTCTGTACGAGGACGAGCCACTGAAGGAATACTACACCCTCATGGAC
  ATCGCCTACATCTACCCCTGGCGGCGGAACGGGCCTCTCCCCCTCAAGTACCGTGTCCAGCCAGCCTGCA
  AGCGGCTCACCCTAGCCACGGTGCCCACCCCCTCCGAGGGCACCAACACCAGCGGGGCGTCCGAGTGTGA
  GTCAGTCAGCGACAAGGCTCCCAGCCCTGCCACCCTGCCAGCCACCTCCTCCTCCCTGCCCAGCCCAGCC
  ACCCCATCCCATGGCTCTCCCAGTTCCCATGGGCCTCCAGCCACCCACCCTACCTCCCCCACTCCCCCTT
  CGACAGCCAGTGGGGCCACCACAGCTGCCAACGGGGGTAGCTTGAACTGCCTGCAGACACCATCCTCCAC
  CAGCAGGGGGCGCAAGATGACTGTCAACGGCGCTCCCGTGCCCCCCTTAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202490 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIR
  SDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPLTEVPNGSNEDRGEVLEQEKGALSDDEIVSLSIEFYEG
  ARDRDEKKGPLENGDGDKEKTGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEVLYEDEPLKEYYTLMD
  IAYIYPWRRNGPLPLKYRVQPACKRLTLATVPTPSEGTNTSGASECESVSDKAPSPATLPATSSSLPSPA
  TPSHGSPSSHGPPATHPTSPTPPSTASGATTAANGGSLNCLQTPSSTSRGRKMTVNGAPVPPLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007144
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007144.3
• RefSeq Size: 2622 bp
• RefSeq ORF: 1035 bp
• Locus ID: 7703
• UniProt ID: P35227
• Cytogenetics: 17q12
• Domains: RING
• Protein Families: Transcription Factors
• MW: 37.8 kDa
• Gene Summary: The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]