TOX4 (NM_014828) Human Tagged ORF Clone

CAT#: RC202171

TOX4 (Myc-DDK-tagged)-Human TOX high mobility group box family member 4 (TOX4)

Product Images

Circular map for RC202171

• 

  Western blot validation of overexpression lysate (Cat# LY415032) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TOX4
• Synonyms: C14orf92; KIAA0737; LCP1; MIG7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202171 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTCCCGGAGGAAATGACAATTACCTGACGATCACAGGGCCTTCGCACCCCTTCCTGTCAGGGG
  CCGAGACATTCCATACACCAAGCTTGGGTGATGAGGAATTTGAAATCCCACCTATCTCCTTGGATTCTGA
  TCCCTCATTGGCTGTCTCAGATGTGGTTGGCCACTTTGATGACCTGGCAGACCCTTCCTCTTCACAGGAT
  GGCAGTTTTTCAGCCCAGTATGGGGTCCAGACATTGGACATGCCTGTGGGCATGACCCATGGCTTGATGG
  AGCAGGGCGGGGGGCTCCTGAGTGGGGGCTTGACCATGGACTTGGACCACTCTATAGGAACTCAGTATAG
  TGCCAACCCACCTGTTACAATTGATGTACCAATGACAGACATGACATCTGGCTTGATGGGGCATAGCCAG
  TTGACCACCATTGATCAGTCAGAACTGAGTTCCCAGCTGGGTTTGAGCCTAGGGGGTGGCACCATCCTGC
  CACCTGCCCAGTCACCTGAAGATCGTCTTTCAACCACCCCTTCACCTACTAGTTCACTTCACGAGGATGG
  TGTTGAGGATTTCCGGAGGCAACTTCCCAGCCAGAAGACAGTCGTGGTGGAAGCAGGGAAAAAGCAGAAG
  GCCCCAAAGAAGAGAAAAAAGAAAGATCCTAATGAACCTCAGAAACCAGTTTCAGCATATGCTTTATTCT
  TTCGTGATACACAGGCTGCCATCAAGGGACAGAATCCTAATGCCACTTTTGGTGAGGTTTCAAAAATTGT
  GGCCTCCATGTGGGATAGTCTTGGAGAGGAGCAAAAACAGGTATATAAGAGGAAAACTGAGGCTGCCAAG
  AAAGAGTATCTGAAGGCACTGGCTGCTTACAAAGACAACCAGGAGTGTCAGGCCACTGTGGAAACAGTGG
  AATTGGATCCAGCACCACCATCACAAACTCCTTCTCCACCTCCTATGGCTACTGTTGACCCAGCATCTCC
  AGCACCAGCTTCAATAGAGCCCCCTGCCCTGTCCCCATCCATTGTTGTTAACTCCACCCTCTCATCCTAT
  GTGGCAAACCAGGCATCTTCTGGAGCTGGGGGTCAGCCCAATATCACCAAGTTGATTATTACCAAACAAA
  TGTTGCCCTCTTCTATTACTATGTCTCAAGGAGGGATGGTTACTGTTATCCCAGCCACAGTGGTGACCTC
  CCGAGGGCTCCAACTAGGCCAAACCAGTACAGCTACTATCCAGCCCAGTCAACAAGCCCAGATTGTCACT
  CGGTCAGTGTTGCAGGCAGCAGCAGCTGCTGCTGCTGCTGCTTCTATGCAACTGCCTCCACCCCGACTAC
  AGCCCCCTCCATTACAACAGATGCCACAGCCCCCGACTCAGCAGCAAGTTACCATTCTGCAGCAGCCTCC
  TCCACTCCAGGCCATGCAACAGCCTCCACCTCAGAAAGTTCGAATCAATTTACAGCAACAGCCTCCTCCT
  CTGCAGATCAAGAGTGTGCCTCTACCCACTTTGAAAATGCAGACTACCTTAGTCCCACCAACTGTGGAAA
  GTAGTCCTGAGCGGCCTATGAACAACAGCCCTGAGGCCCATACAGTGGAGGCACCTTCTCCTGAGACTAT
  CTGTGAGATGATCACAGATGTAGTTCCTGAGGTTGAGTCTCCTTCTCAGATGGATGTTGAATTGGTGAGT
  GGGTCTCCTGTGGCACTCTCACCCCAGCCTCGATGTGTGAGGTCTGGTTGTGAGAACCCTCCCATTGTGA
  GTAAGGACTGGGACAATGAATACTGCAGCAATGAGTGTGTGGTGAAGCACTGCAGGGATGTATTCTTGGC
  CTGGGTAGCCTCTAGAAATTCAAACACAGTGGTGTTTGTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202171 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEFPGGNDNYLTITGPSHPFLSGAETFHTPSLGDEEFEIPPISLDSDPSLAVSDVVGHFDDLADPSSSQD
  GSFSAQYGVQTLDMPVGMTHGLMEQGGGLLSGGLTMDLDHSIGTQYSANPPVTIDVPMTDMTSGLMGHSQ
  LTTIDQSELSSQLGLSLGGGTILPPAQSPEDRLSTTPSPTSSLHEDGVEDFRRQLPSQKTVVVEAGKKQK
  APKKRKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPNATFGEVSKIVASMWDSLGEEQKQVYKRKTEAAK
  KEYLKALAAYKDNQECQATVETVELDPAPPSQTPSPPPMATVDPASPAPASIEPPALSPSIVVNSTLSSY
  VANQASSGAGGQPNITKLIITKQMLPSSITMSQGGMVTVIPATVVTSRGLQLGQTSTATIQPSQQAQIVT
  RSVLQAAAAAAAAASMQLPPPRLQPPPLQQMPQPPTQQQVTILQQPPPLQAMQQPPPQKVRINLQQQPPP
  LQIKSVPLPTLKMQTTLVPPTVESSPERPMNNSPEAHTVEAPSPETICEMITDVVPEVESPSQMDVELVS
  GSPVALSPQPRCVRSGCENPPIVSKDWDNEYCSNECVVKHCRDVFLAWVASRNSNTVVFVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014828
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014828.1, NP_055643.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 1866 bp
• Locus ID: 9878
• UniProt ID: O94842
• Cytogenetics: 14q11.2
• Domains: HMG
• Protein Families: Transcription Factors
• MW: 66.2 kDa
• Gene Summary: Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.[UniProtKB/Swiss-Prot Function]