KLF10 (NM_001032282) Human Tagged ORF Clone

CAT#: RC202028

KLF10 (Myc-DDK-tagged)-Human Kruppel-like factor 10 (KLF10), transcript variant 2

Product Images

Circular map for RC202028

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLF10
• Synonyms: EGR-alpha; EGRA; TIEG; TIEG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202028 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAAGAATGGAAATGATTTCTGAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAG
  AGAAAAGTGATTTTGAAGCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAA
  ATACGTTGAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGAACA
  CCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGACTTTGAACCCTCTC
  AAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAGTCACTCTCAGATACTGCCAAACC
  TCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGCCCAGTATCTGCCCCCAAACTCCCCAAAGCTCAG
  GCAACAAGTGTGATTCGTCATACAGCTGATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAG
  CCAGCATCCTCAACTATCAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAA
  GAACATACCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTTGAT
  GAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATCTGCAGGTCTCAGC
  CAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCTGCAGTATCTGCAGGGGGAGTGCC
  ACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACCCTGTTGTGACAACAGTCGTTCCC
  AGCACTCCTCCCAGCCAGCCACCAGCCGTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAG
  GCGCTGTCATGTTTGTGGTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGG
  CACCAGACTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAGATT
  GATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATACTTTAAAAGTTCCC
  ATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGCTGTAGCTGGAAAGGTTGTGAAAG
  GAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGGCGAACCCACACGGGTGAGAAGAAATTTGCGTGC
  CCCATGTGTGACCGGCGGTTCATGAGGAGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCA
  AGAAGCTACCAAACTGGCAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCC
  CACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202028 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEENLLPGT
  PDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIAAPFKEEEKSPVSAPKLPKAQ
  ATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHLNVEAARKNIPCAAVSPNRSKCERNTVADVD
  EKASAALYDFSVPSSETVICRSQPAPVSPQQKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVP
  STPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQI
  DSSRIRSHICSHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFAC
  PMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032282
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032282.3
• RefSeq Size: 3273 bp
• RefSeq ORF: 1410 bp
• Locus ID: 7071
• UniProt ID: Q13118
• Cytogenetics: 8q22.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 51.4 kDa
• Gene Summary: This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]