U1A (SNRPA) (NM_004596) Human Tagged ORF Clone
CAT#: RC201818
SNRPA (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein polypeptide A (SNRPA)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004596" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | U1A |
Synonyms | Mud1; U1-A; U1A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTTCCCGAGACCCGCCCTAACCACACTATTTATATCAACAACCTCAATGAGAAGATCAAGAAGG ATGAGCTAAAAAAGTCCCTGTACGCCATCTTCTCCCAGTTTGGCCAGATCCTGGATATCCTGGTATCACG GAGCCTGAAGATGAGGGGCCAGGCCTTTGTCATCTTCAAGGAGGTCAGCAGCGCCACCAACGCCCTGCGC TCCATGCAGGGTTTCCCTTTCTATGACAAACCTATGCGTATCCAGTATGCCAAGACCGACTCAGATATCA TTGCCAAGATGAAAGGCACCTTCGTGGAGCGGGACCGCAAGCGGGAGAAGAGGAAGCCCAAGAGCCAGGA GACCCCGGCCACCAAGAAGGCTGTGCAAGGCGGGGGAGCCACCCCCGTGGTGGGGGCTGTCCAGGGGCCT GTCCCGGGCATGCCGCCGATGACTCAGGCGCCCCGCATTATGCACCACATGCCGGGCCAGCCGCCCTACA TGCCGCCCCCTGGTATGATCCCCCCGCCAGGCCTTGCACCTGGCCAGATCCCACCAGGGGCCATGCCCCC GCAGCAGCTTATGCCAGGACAGATGCCCCCTGCCCAGCCTCTTTCTGAGAATCCACCGAATCACATCTTG TTCCTCACCAACCTGCCAGAGGAGACCAACGAGCTCATGCTGTCCATGCTTTTCAATCAGTTCCCTGGCT TCAAGGAGGTCCGTCTGGTACCCGGGCGGCATGACATCGCCTTCGTGGAGTTTGACAATGAGGTACAGGC AGGGGCAGCTCGCGATGCCCTGCAGGGCTTTAAGATCACGCAGAACAACGCCATGAAGATCTCCTTTGCC AAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201818 protein sequence
Red=Cloning site Green=Tags(s) MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALR SMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGP VPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHIL FLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFA KK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004596 |
ORF Size | 846 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004596.5 |
RefSeq Size | 1646 bp |
RefSeq ORF | 849 bp |
Locus ID | 6626 |
UniProt ID | P09012 |
Cytogenetics | 19q13.2 |
Domains | RRM |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | Spliceosome |
MW | 31.3 kDa |
Gene Summary | The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201818L3 | Lenti ORF clone of Human small nuclear ribonucleoprotein polypeptide A (SNRPA), Myc-DDK-tagged |
USD 600.00 |
|
RC201818L4 | Lenti ORF clone of Human small nuclear ribonucleoprotein polypeptide A (SNRPA), mGFP tagged |
USD 600.00 |
|
RG201818 | SNRPA (tGFP-tagged) - Human small nuclear ribonucleoprotein polypeptide A (SNRPA) |
USD 500.00 |
|
SC320243 | SNRPA (untagged)-Human small nuclear ribonucleoprotein polypeptide A (SNRPA) |
USD 300.00 |
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