ZNF416 (NM_017879) Human Tagged ORF Clone

CAT#: RC201632

ZNF416 (Myc-DDK-tagged)-Human zinc finger protein 416 (ZNF416). Note: ORF is codon optimized

Product Images

Circular map for RC201632

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF416
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201632 ORF sequence, codon optimized.
  Due to the complexity of NM_017879, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCCGTTCTGCGGGACAGCACTAGTGTTCCCGTCACAGCTGAGGCAAAGCTGATGGGCTTTA
  CTCAGGGCTGTGTCACGTTCGAAGACGTAGCCATCTATTTTAGTCAGGAGGAATGGGGACTGCTGGATGA
  GGCCCAAAGACTCCTTTATCGAGACGTGATGCTGGAGAACTTCGCACTTATCACAGCCCTGGTATGTTGG
  CACGGAATGGAGGACGAAGAGACTCCGGAACAGAGCGTAAGCGTCGAAGGGGTCCCGCAAGTACGAACCC
  CCGAAGCTTCCCCCAGCACACAAAAGATCCAGAGTTGCGACATGTGCGTGCCATTTCTCACAGATATACT
  GCACCTGACTGATCTGCCCGGCCAGGAGCTTTACTTGACGGGCGCATGTGCCGTGTTCCACCAGGACCAG
  AAGCACCACTCCGCTGAAAAGCCCTTGGAGTCCGATATGGACAAGGCTAGCTTTGTTCAATGTTGCCTTT
  TTCATGAGTCCGGTATGCCTTTTACAAGTTCTGAGGTCGGAAAGGACTTTCTGGCTCCCCTTGGCATCCT
  TCAGCCTCAGGCGATAGCCAATTACGAAAAACCAAATAAGATATCTAAATGTGAGGAAGCTTTTCACGTG
  GGGATTAGCCACTACAAATGGTCCCAATGCAGAAGAGAGAGTTCCCATAAACATACTTTCTTTCACCCTA
  GAGTTTGCACGGGCAAACGGCTGTACGAATCTAGCAAATGCGGAAAGGCATGCTGCTGCGAGTGTTCCCT
  TGTGCAACTGCAGCGAGTGCACCCCGGAGAAAGGCCCTATGAATGCTCAGAGTGCGGCAAGAGCTTTTCT
  CAGACATCCCACCTGAACGACCACCGGAGAATCCACACAGGGGAAAGACCGTATGTGTGCGGACAATGCG
  GTAAATCATTCTCCCAGCGCGCAACCCTCATCAAGCACCATAGAGTGCACACAGGAGAGCGGCCGTACGA
  ATGTGGAGAATGCGGGAAGAGTTTCTCCCAGAGTTCCAACCTGATCGAGCACTGTAGGATCCACACCGGG
  GAGCGCCCATACGAGTGCGACGAGTGCGGCAAAGCTTTCGGCTCCAAGAGCACCCTCGTGAGACATCAAA
  GAACCCACACGGGTGAGAAGCCCTACGAATGCGGAGAATGCGGCAAACTGTTTCGGCAGTCTTTCAGCCT
  GGTGGTGCACCAGAGGATCCACACAACCGCTCGGCCTTACGAATGTGGGCAATGCGGTAAGTCTTTCTCT
  CTGAAATGCGGGTTGATCCAGCACCAGCTTATTCACTCCGGCGCCCGACCATTTGAGTGCGATGAGTGCG
  GTAAGTCTTTTTCTCAACGCACTACACTCAACAAACATCACAAGGTTCACACAGCGGAGCGACCCTATGT
  GTGCGGAGAGTGCGGCAAAGCTTTCATGTTCAAGTCCAAACTGGTCAGACATCAACGGACTCATACAGGC
  GAACGCCCCTTCGAGTGCAGCGAATGCGGCAAGTTTTTCCGCCAAAGTTACACCTTGGTGGAGCATCAGA
  AAATTCACACTGGTCTGCGACCCTACGACTGCGGTCAGTGCGGAAAGTCTTTCATTCAGAAGAGTTCACT
  CATCCAGCATCAAGTGGTTCACACGGGAGAACGCCCTTATGAATGTGGAAAGTGCGGCAAGAGCTTCACG
  CAGCATTCCGGACTTATCCTCCATCGGAAGAGTCACACAGTGGAAAGGCCCCGAGATTCAAGCAAATGTG
  GGAAGCCCTACAGTCCCAGATCTAATATTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201632 representing NM_017879
  Red=Cloning site Green=Tags(s)
  
  MAAAVLRDSTSVPVTAEAKLMGFTQGCVTFEDVAIYFSQEEWGLLDEAQRLLYRDVMLENFALITALVCW
  HGMEDEETPEQSVSVEGVPQVRTPEASPSTQKIQSCDMCVPFLTDILHLTDLPGQELYLTGACAVFHQDQ
  KHHSAEKPLESDMDKASFVQCCLFHESGMPFTSSEVGKDFLAPLGILQPQAIANYEKPNKISKCEEAFHV
  GISHYKWSQCRRESSHKHTFFHPRVCTGKRLYESSKCGKACCCECSLVQLQRVHPGERPYECSECGKSFS
  QTSHLNDHRRIHTGERPYVCGQCGKSFSQRATLIKHHRVHTGERPYECGECGKSFSQSSNLIEHCRIHTG
  ERPYECDECGKAFGSKSTLVRHQRTHTGEKPYECGECGKLFRQSFSLVVHQRIHTTARPYECGQCGKSFS
  LKCGLIQHQLIHSGARPFECDECGKSFSQRTTLNKHHKVHTAERPYVCGECGKAFMFKSKLVRHQRTHTG
  ERPFECSECGKFFRQSYTLVEHQKIHTGLRPYDCGQCGKSFIQKSSLIQHQVVHTGERPYECGKCGKSFT
  QHSGLILHRKSHTVERPRDSSKCGKPYSPRSNIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017879
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017879.1, NP_060349.1
• RefSeq Size: 2545 bp
• RefSeq ORF: 1785 bp
• Locus ID: 55659
• UniProt ID: Q9BWM5
• Cytogenetics: 19q13.43
• MW: 67.2 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]