TRMT61B (NM_017910) Human Tagged ORF Clone

CAT#: RC201499

TRMT61B (Myc-DDK-tagged)-Human tRNA methyltransferase 61 homolog B (S. cerevisiae) (TRMT61B)

Product Images

Circular map for RC201499

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  Western blot validation of overexpression lysate (Cat# LY413456) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201499 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRMT61B protein (Cat# TP301499). The protein was produced from HEK293T cells transfected with TRMT61B cDNA clone (Cat# RC201499) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRMT61B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201499 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAATGGCATGGTGCCGCGGTCCTGTCTTGCTGTGCCTGCGGCAGGGGCTCGGAACCAATTCATTCC
  TGCACGGCCTGGGGCAGGAGCCCTTCGAGGGAGCTCGGTCACTGTGTTGCAGGTCCTCGCCTAGAGACCT
  GCGAGATGGAGAAAGAGAGCACGAGGCGGCACAAAGGAAAGCCCCAGGAGCAGAGTCTTGCCCATCTCTC
  CCTCTGAGCATCTCGGACATTGGGACTGGATGTCTTTCGTCACTGGAAAACCTCAGACTGCCGACGCTGC
  GGGAAGAGTCATCACCTCGAGAGCTCGAGGACTCGAGCGGAGACCAGGGCCGGTGCGGTCCCACACACCA
  GGGATCCGAGGATCCTTCGATGCTCTCGCAGGCCCAGTCCGCTATCGAGGTCGAAGAGCGTCACGTCTCC
  CCTTCTTGTTCAACTTCCAGAGAGAGACCCTTTCAGGCTGGGGAGCTGATTTTAGCTGAGACTGGGGAGG
  GAGAAACAAAATTTAAGAAATTATTTAGGTTGAACAACTTCGGACTCTTAAATAGTAACTGGGGGGCAGT
  CCCGTTCGGCAAGATCGTGGGGAAGTTCCCCGGCCAGATACTGAGGAGTTCCTTCGGTAAGCAGTACATG
  CTGAGGAGGCCAGCCTTGGAAGACTATGTAGTATTGATGAAAAGAGGGACTGCCATAACATTCCCAAAGG
  ATATTAATATGATTCTCTCAATGATGGATATCAACCCAGGTGATACTGTTTTGGAAGCTGGCTCAGGCTC
  TGGTGGAATGAGCTTATTTTTATCCAAAGCAGTTGGATCACAAGGACGAGTCATAAGTTTTGAGGTACGA
  AAAGACCACCATGATCTGGCTAAGAAGAATTACAAACACTGGCGTGATTCATGGAAATTAAGTCATGTAG
  AAGAGTGGCCAGACAATGTGGATTTTATTCATAAGGACATTTCAGGAGCAACCGAAGACATAAAATCTTT
  AACATTTGACGCAGTAGCTTTGGATATGTTAAATCCTCATGTTACTTTGCCTGTTTTTTACCCACATCTT
  AAGCATGGTGGTGTATGTGCTGTATATGTAGTAAACATCACACAGGTTATTGAACTTTTAGATGGAATTC
  GCACCTGTGAACTTGCTCTTTCATGTGAAAAGATAAGCGAGGTCATTGTCAGAGATTGGTTGGTTTGCCT
  TGCAAAACAGAAAAATGGAATTTTAGCTCAAAAAGTAGAATCTAAAATCAACACAGATGTACAACTAGAT
  TCTCAAGAGAAAATTGGAGTTAAAGGTGAGCTGTTTCAAGAGGATGACCATGAAGAATCGCATTCTGATT
  TTCCATATGGATCATTTCCCTATGTTGCTAGACCAGTACACTGGCAACCTGGTCATACAGCTTTTCTTGT
  CAAGTTGAGGAAGGTCAAACCACAACTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201499 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLMAWCRGPVLLCLRQGLGTNSFLHGLGQEPFEGARSLCCRSSPRDLRDGEREHEAAQRKAPGAESCPSL
  PLSISDIGTGCLSSLENLRLPTLREESSPRELEDSSGDQGRCGPTHQGSEDPSMLSQAQSAIEVEERHVS
  PSCSTSRERPFQAGELILAETGEGETKFKKLFRLNNFGLLNSNWGAVPFGKIVGKFPGQILRSSFGKQYM
  LRRPALEDYVVLMKRGTAITFPKDINMILSMMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVR
  KDHHDLAKKNYKHWRDSWKLSHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHL
  KHGGVCAVYVVNITQVIELLDGIRTCELALSCEKISEVIVRDWLVCLAKQKNGILAQKVESKINTDVQLD
  SQEKIGVKGELFQEDDHEESHSDFPYGSFPYVARPVHWQPGHTAFLVKLRKVKPQLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017910
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017910.2, NP_060380.2
• RefSeq Size: 1856 bp
• RefSeq ORF: 1434 bp
• Locus ID: 55006
• UniProt ID: Q9BVS5
• Cytogenetics: 2p23.2
• Protein Families: Druggable Genome
• MW: 53 kDa
• Gene Summary: Methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (PubMed:23097428). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function (PubMed:27631568). In addition to tRNA N(1)-methyltransferase activity, also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA, leading to interfere with mitochondrial translation (PubMed:29107537).[UniProtKB/Swiss-Prot Function]