TRIP (TRAIP) (NM_005879) Human Tagged ORF Clone

CAT#: RC201252

TRAIP (Myc-DDK-tagged)-Human TRAF interacting protein (TRAIP)

Product Images

Circular map for RC201252

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRAIP (Cat# RC201252, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAIP(Cat# TA800086). Positive lysates LY416999 (100ug) and LC416999 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416999) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201252 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIP
• Synonyms: RNF206; SCKL9; TRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201252 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTATCCGTGCTCTGTGCACTATCTGCTCCGACTTCTTCGATCACTCCCGCGACGTGGCCGCCATCC
  ACTGCGGCCACACCTTCCACTTGCAGTGCCTAATTCAGTGGTTTGAGACAGCACCAAGTCGGACCTGCCC
  ACAGTGCCGAATCCAGGTTGGCAAAAGAACCATTATCAATAAGCTCTTCTTTGATCTTGCCCAGGAGGAG
  GAGAATGTCTTGGATGCAGAATTCTTAAAGAATGAACTGGACAATGTCAGAGCCCAGCTTTCCCAGAAAG
  ACAAGGAGAAACGAGACAGCCAGGTCATCATCGACACTCTGCGGGATACGCTGGAAGAACGCAATGCTAC
  TGTGGTATCTCTGCAGCAGGCCTTGGGCAAGGCCGAGATGCTGTGCTCCACACTGAAAAAGCAGATGAAG
  TACTTAGAGCAGCAGCAGGATGAGACCAAACAAGCACAAGAGGAGGCCCGCCGGCTCAGGAGCAAGATGA
  AGACCATGGAGCAGATTGAGCTTCTACTCCAGAGCCAGCGCCCTGAGGTGGAGGAGATGATCCGAGACAT
  GGGTGTGGGACAGTCAGCGGTGGAACAGCTGGCTGTGTACTGTGTGTCTCTCAAGAAAGAGTACGAGAAT
  CTAAAAGAGGCACGGAAGGCCTCAGGGGAGGTGGCTGACAAGCTGAGGAAGGATTTGTTTTCCTCCAGAA
  GCAAGTTGCAGACAGTCTACTCTGAATTGGATCAGGCCAAGTTAGAACTGAAGTCAGCCCAGAAGGACTT
  ACAGAGTGCTGACAAGGAAATCATGAGCCTGAAAAAGAAGCTAACGATGCTGCAGGAAACCTTGAACCTG
  CCACCAGTGGCCAGTGAGACTGTCGACCGCCTGGTTTTAGAGAGCCCAGCCCCTGTGGAGGTGAATCTGA
  AGCTCCGCCGGCCATCCTTCCGTGATGATATTGATCTCAATGCTACCTTTGATGTGGATACTCCCCCAGC
  CCGGCCCTCCAGCTCCCAGCATGGTTACTACGAAAAACTTTGCCTAGAGAAGTCACACTCCCCAATTCAG
  GATGTCCCCAAGAAGATATGCAAAGGCCCCAGGAAGGAGTCCCAGCTCTCACTGGGTGGCCAGAGCTGTG
  CAGGAGAGCCAGATGAGGAACTGGTTGGTGCCTTCCCTATTTTTGTCCGGAATGCCATCCTAGGCCAGAA
  ACAGCCCAAGAGGCCCAGGTCAGAGTCCTCTTGCAGCAAAGATGTGGTAAGGACAGGCTTCGATGGGCTC
  GGTGGCCGGACAAAATTCATCCAGCCTACTGACACAGTCATGATCCGCCCATTGCCTGTTAAGCCCAAGA
  CCAAGGTTAAGCAGAGGGTGAGGGTGAAGACAGTGCCTTCTCTCTTCCAGGCCAAGCTGGACACCTTCCT
  GTGGTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201252 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEE
  ENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMK
  YLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYEN
  LKEARKASGEVADKLRKDLFSSRSKLQTVYSELDQAKLELKSAQKDLQSADKEIMSLKKKLTMLQETLNL
  PPVASETVDRLVLESPAPVEVNLKLRRPSFRDDIDLNATFDVDTPPARPSSSQHGYYEKLCLEKSHSPIQ
  DVPKKICKGPRKESQLSLGGQSCAGEPDEELVGAFPIFVRNAILGQKQPKRPRSESSCSKDVVRTGFDGL
  GGRTKFIQPTDTVMIRPLPVKPKTKVKQRVRVKTVPSLFQAKLDTFLWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005879
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005879.3
• RefSeq Size: 2042 bp
• RefSeq ORF: 1410 bp
• Locus ID: 10293
• UniProt ID: Q9BWF2
• Cytogenetics: 3p21.31
• Domains: RING
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 53.3 kDa
• Gene Summary: This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis. [provided by RefSeq, Jul 2008]