Integrin beta 5 (ITGB5) (NM_002213) Human Tagged ORF Clone
CAT#: RC200731
- TrueORF®
ITGB5 (Myc-DDK-tagged)-Human integrin, beta 5 (ITGB5)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_002213" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Integrin beta 5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGGGCCCCGGCGCCGCTGTACGCCTGCCTCCTGGGGCTCTGCGCGCTCCTGCCCCGGCTCGCAG GTCTCAACATATGCACTAGTGGAAGTGCCACCTCATGTGAAGAATGTCTGCTAATCCACCCAAAATGTGC CTGGTGCTCCAAAGAGGACTTCGGAAGCCCACGGTCCATCACCTCTCGGTGTGATCTGAGGGCAAACCTT GTCAAAAATGGCTGTGGAGGTGAGATAGAGAGCCCAGCCAGCAGCTTCCATGTCCTGAGGAGCCTGCCCC TCAGCAGCAAGGGTTCGGGCTCTGCAGGCTGGGACGTCATTCAGATGACACCACAGGAGATTGCCGTGAA CCTCCGGCCCGGTGACAAGACCACCTTCCAGCTACAGGTTCGCCAGGTGGAGGACTATCCTGTGGACCTG TACTACCTGATGGACCTCTCCCTGTCCATGAAGGATGACTTGGACAATATCCGGAGCCTGGGCACCAAAC TCGCGGAGGAGATGAGGAAGCTCACCAGCAACTTCCGGTTGGGATTTGGGTCTTTTGTTGATAAGGACAT CTCTCCTTTCTCCTACACGGCACCGAGGTACCAGACCAATCCGTGCATTGGTTACAAGTTGTTTCCAAAT TGCGTCCCCTCCTTTGGGTTCCGCCATCTGCTGCCTCTCACAGACAGAGTGGACAGCTTCAATGAGGAAG TTCGGAAACAGAGGGTGTCCCGGAACCGAGATGCCCCTGAGGGGGGCTTTGATGCAGTACTCCAGGCAGC CGTCTGCAAGGAGAAGATTGGCTGGCGAAAGGATGCACTGCATTTGCTGGTGTTCACAACAGATGATGTG CCCCACATCGCATTGGATGGAAAATTGGGAGGCCTGGTGCAGCCACACGATGGCCAGTGCCACCTGAACG AGGCCAACGAGTACACTGCATCCAACCAGATGGACTATCCATCCCTTGCCTTGCTTGGAGAGAAATTGGC AGAGAACAACATCAACCTCATCTTTGCAGTGACAAAAAACCATTATATGCTGTACAAGAATTTTACAGCC CTGATACCTGGAACAACGGTGGAGATTTTAGATGGAGACTCCAAAAATATTATTCAACTGATTATTAATG CATACAATAGTATCCGGTCTAAAGTGGAGTTGTCAGTCTGGGATCAGCCTGAGGATCTTAATCTCTTCTT TACTGCTACCTGCCAAGATGGGGTATCCTATCCTGGTCAGAGGAAGTGTGAGGGTCTGAAGATTGGGGAC ACGGCATCTTTTGAAGTATCATTGGAGGCCCGAAGCTGTCCCAGCAGACACACGGAGCATGTGTTTGCCC TGCGGCCGGTGGGATTCCGGGACAGCCTGGAGGTGGGGGTCACCTACAACTGCACGTGCGGCTGCAGCGT GGGGCTGGAACCCAACAGTGCCAGGTGCAACGGGAGCGGGACCTATGTCTGCGGCCTGTGTGAGTGCAGC CCCGGCTACCTGGGCACCAGGTGCGAGTGCCAGGATGGGGAGAACCAGAGCGTGTACCAGAACCTGTGCC GGGAGGCAGAGGGCAAGCCACTGTGCAGCGGGCGTGGGGACTGCAGCTGCAACCAGTGCTCCTGCTTCGA GAGCGAGTTCGGCAAGATCTATGGGCCTTTCTGTGAGTGCGACAACTTCTCCTGTGCCAGGAACAAGGGA GTCCTCTGCTCAGGCCATGGCGAGTGTCACTGCGGGGAATGCAAGTGCCATGCAGGTTACATCGGGGACA ACTGTAACTGCTCGACAGACATCAGCACATGCCGGGGCAGAGATGGCCAGATCTGCAGCGAGCGTGGGCA CTGTCTCTGTGGGCAGTGCCAATGCACGGAGCCGGGGGCCTTTGGGGAGATGTGTGAGAAGTGCCCCACC TGCCCGGATGCATGCAGCACCAAGAGAGATTGCGTCGAGTGCCTGCTGCTCCACTCTGGGAAACCTGACA ACCAGACCTGCCACAGCCTATGCAGGGATGAGGTGATCACATGGGTGGACACCATCGTGAAAGATGACCA GGAGGCTGTGCTATGTTTCTACAAAACCGCCAAGGACTGCGTCATGATGTTCACCTATGTGGAGCTCCCC AGTGGGAAGTCCAACCTGACCGTCCTCAGGGAGCCAGAGTGTGGAAACACCCCCAACGCCATGACCATCC TCCTGGCTGTGGTCGGTAGCATCCTCCTTGTTGGGCTTGCACTCCTGGCTATCTGGAAGCTGCTTGTCAC CATCCACGACCGGAGGGAGTTTGCAAAGTTTCAGAGCGAGCGATCCAGGGCCCGCTATGAAATGGCTTCA AATCCATTATACAGAAAGCCTATCTCCACGCACACTGTGGACTTCACCTTCAACAAGTTCAACAAATCCT ACAATGGCACTGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200731 protein sequence
Red=Cloning site Green=Tags(s) MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANL VKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDL YYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPN CVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDV PHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTA LIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGD TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECS PGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEFGKIYGPFCECDNFSCARNKG VLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQICSERGHCLCGQCQCTEPGAFGEMCEKCPT CPDACSTKRDCVECLLLHSGKPDNQTCHSLCRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELP SGKSNLTVLREPECGNTPNAMTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMAS NPLYRKPISTHTVDFTFNKFNKSYNGTVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002213 |
ORF Size | 2397 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002213.3, NP_002204.2 |
RefSeq Size | 3392 bp |
RefSeq ORF | 2400 bp |
Locus ID | 3693 |
UniProt ID | P18084 |
Cytogenetics | 3q21.2 |
Domains | VWA, INB, PSI, EGF |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton |
MW | 88.1 kDa |
Gene Summary | This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin. [provided by RefSeq, Aug 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200731L1 | Lenti ORF clone of Human integrin, beta 5 (ITGB5), Myc-DDK-tagged |
USD 1,397.00 |
|
RC200731L2 | Lenti ORF clone of Human integrin, beta 5 (ITGB5), mGFP tagged |
USD 1,397.00 |
|
RC200731L3 | Lenti ORF clone of Human integrin, beta 5 (ITGB5), Myc-DDK-tagged |
USD 1,397.00 |
|
RC200731L4 | Lenti ORF clone of Human integrin, beta 5 (ITGB5), mGFP tagged |
USD 1,397.00 |
|
RG200731 | ITGB5 (tGFP-tagged) - Human integrin, beta 5 (ITGB5) |
USD 1,297.00 |
|
SC118751 | ITGB5 (untagged)-Human integrin, beta 5 (ITGB5) |
USD 1,098.00 |
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