GPAA1 (NM_003801) Human Tagged Lenti ORF Clone
CAT#: RC200271L3
- LentiORF®
Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), Myc-DDK-tagged
"NM_003801" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GPAA1 |
Synonyms | GAA1; GPIBD15; hGAA1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200271).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003801 |
ORF Size | 1863 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003801.3 |
RefSeq Size | 2105 bp |
RefSeq ORF | 1866 bp |
Locus ID | 8733 |
UniProt ID | O43292 |
Cytogenetics | 8q24.3 |
Domains | Gaa1 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
MW | 67.6 kDa |
Gene Summary | Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200271 | GPAA1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1) |
USD 578.00 |
|
RC200271L1 | Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), Myc-DDK-tagged |
USD 878.00 |
|
RC200271L2 | Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), mGFP tagged |
USD 878.00 |
|
RC200271L4 | Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), mGFP tagged |
USD 878.00 |
|
RG200271 | GPAA1 (tGFP-tagged) - Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1) |
USD 778.00 |
|
SC319411 | GPAA1 (untagged)-Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1) |
USD 579.00 |
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