Bcl rambo (BCL2L13) (NM_015367) Human Tagged ORF Clone

CAT#: RC200042

BCL2L13 (Myc-DDK-tagged)-Human BCL2-like 13 (apoptosis facilitator) (BCL2L13), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC200042

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  Western blot validation of overexpression lysate (Cat# LY414596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200042 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BCL2L13 protein (Cat# TP300042). The protein was produced from HEK293T cells transfected with BCL2L13 cDNA clone (Cat# RC200042) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcl rambo
• Synonyms: BCL-RAMBO; Bcl2-L-13; MIL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200042 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCTTCTACTGTGCCTCTGGGATTTCACTATGAAACAAAGTATGTTGTTCTCAGCTACTTGG
  GACTCCTCTCTCAAGAGAAGCTGCAAGAGCAACATCTTTCCTCACCCCAAGGGGTTCAACTAGATATAGC
  TTCACAATCTCTGGATCAAGAAATTTTATTAAAAGTTAAAACTGAAATTGAAGAAGAGCTAAAATCTCTG
  GACAAAGAAATTTCTGAAGCCTTCACCAGCACAGGCTTTGACCGTCACACTTCTCCAGTGTTCAGCCCTG
  CCAATCCAGAAAGCTCAATGGAAGACTGCTTGGCCCATCTTGGAGAAAAAGTGTCCCAGGAACTGAAAGA
  GCCTCTCCATAAAGCATTGCAAATGCTCCTGAGCCAGCCAGTGACATATCAGGCATTTCGGGAATGTACA
  CTGGAGACCACAGTTCATGCCAGCGGCTGGAATAAGATTTTGGTGCCTCTGGTTTTGCTACGACAAATGC
  TTTTGGAATTGACAAGACGTGGTCAAGAACCTTTGAGCGCACTGCTGCAGTTTGGCGTGACATACCTGGA
  GGACTATTCGGCAGAGTACATCATTCAGCAAGGTGGCTGGGGCACTGTGTTTAGTCTTGAGTCAGAGGAG
  GAGGAATACCCTGGAATCACTGCAGAAGATAGCAATGACATTTACATCCTGCCCAGCGACAACTCTGGAC
  AAGTCAGTCCCCCAGAGTCTCCAACTGTGACCACTTCCTGGCAGTCTGAGAGCTTACCTGTGTCACTGTC
  AGCTAGCCAGAGTTGGCACACAGAAAGCCTGCCAGTGTCACTAGGCCCTGAGTCCTGGCAGCAGATTGCA
  ATGGATCCTGAAGAAGTGAAAAGCTTAGACAGCAACGGAGCTGGAGAGAAGAGTGAGAACAACTCCTCTA
  ATTCTGACATTGTGCACGTGGAGAAAGAAGAGGTGCCCGAGGGCATGGAAGAGGCTGCTGTGGCTTCTGT
  GGTCTTGCCAGCGCGGGAGCTGCAAGAGGCACTTCCTGAAGCCCCAGCTCCCTTGCTTCCACATATCACT
  GCCACCTCCCTGCTGGGGACAAGGGAATCTGACACAGAAGTGATCACAGTTGAGAAATCCAGCCCTGCTA
  CATCTCTGTTTGTAGAACTTGATGAAGAAGAGGTGAAAGCAGCAACAACTGAACCTACTGAAGTGGAGGA
  GGTGGTCCCCGCACTGGAACCCACAGAAACGCTGCTGAGTGAGAAGGAGATAAACGCAAGGGAAGAGAGC
  CTTGTGGAAGAGCTGTCCCCTGCCAGCGAGAAGAAGCCCGTGCCGCCGTCTGAGGGCAAGTCTAGACTGT
  CCCCCGCCGGTGAGATGAAGCCCATGCCGCTGTCTGAGGGCAAGTCTATACTGCTGTTTGGAGGGGCTGC
  TGCTGTTGCCATCCTGGCAGTGGCCATCGGGGTAGCCCTGGCTCTGAGAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200042 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSSTVPLGFHYETKYVVLSYLGLLSQEKLQEQHLSSPQGVQLDIASQSLDQEILLKVKTEIEEELKSL
  DKEISEAFTSTGFDRHTSPVFSPANPESSMEDCLAHLGEKVSQELKEPLHKALQMLLSQPVTYQAFRECT
  LETTVHASGWNKILVPLVLLRQMLLELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGWGTVFSLESEE
  EEYPGITAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIA
  MDPEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEVPEGMEEAAVASVVLPARELQEALPEAPAPLLPHIT
  ATSLLGTRESDTEVITVEKSSPATSLFVELDEEEVKAATTEPTEVEEVVPALEPTETLLSEKEINAREES
  LVEELSPASEKKPVPPSEGKSRLSPAGEMKPMPLSEGKSILLFGGAAAVAILAVAIGVALALRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015367
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015367.4
• RefSeq Size: 5132 bp
• RefSeq ORF: 1458 bp
• Locus ID: 23786
• UniProt ID: Q9BXK5
• Cytogenetics: 22q11.21
• Domains: Bcl-2
• Protein Families: Druggable Genome, Transmembrane
• MW: 52.7 kDa
• Gene Summary: This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]