Map4k4 (NM_001252200) Mouse Tagged ORF Clone

CAT#: MR231754

Map4k4 (myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1

Product Images

Circular map for MR231754

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map4k4
• Synonyms: 9430080K19Rik; AU043147; AU045934; AW046177; HGK; Nik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231754 representing NM_001252200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACGACTCTCCCGCGAAGAGCCTGGTGGACATTGACCTGTCGTCCCTGCGGGACCCTGCTGGGA
  TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC
  GGGTCAGCTGGCCGCCATCAAGGTTATGGACGTCACCGAGGATGAAGAGGAAGAAATCAAACTGGAGATA
  AATATGCTGAAGAAGTATTCTCATCATCGAAATATTGCCACGTACTATGGTGCTTTCATTAAGAAGAGCC
  CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTTTGTGGGGCTGGGTCCATCACAGACCTTGT
  GAAGAACACCAAAGGGAACACTCTCAAAGAAGACTGGATTGCTTACATCTCCAGGGAAATCCTCAGGGGA
  TTGGCACATCTCCATATTCACCACGTTATTCACCGAGATATCAAGGGCCAAAATGTGCTGCTGACCGAGA
  ATGCTGAGGTGAAACTTGTTGATTTTGGTGTAAGCGCTCAGCTGGACAGGACGGTTGGACGGAGAAATAC
  GTTCATAGGCACACCCTACTGGATGGCTCCAGAGGTCATCGCCTGTGATGAGAACCCAGACGCCACTTAC
  GACTACAGAAGTGACCTCTGGTCCTGTGGCATCACAGCCATCGAGATGGCTGAAGGGGCCCCCCCTCTCT
  GTGACATGCATCCAATGAGAGCGCTGTTTCTCATCCCCAGAAACCCTCCTCCCAGGCTGAAGTCAAAAAA
  ATGGTCAAAGAAATTTTTCAGCTTTATAGAAGGCTGTCTGGTGAAGAATTACATGCAGCGGCCCTCTACA
  GAGCAACTTTTAAAACACCCTTTCATAAGGGATCAGCCCAATGAAAGGCAGGTTCGAATCCAGCTTAAGG
  ATCACATAGACCGGACCAGAAAGAAGAGAGGCGAGAAAGATGAGACGGAGTACGAGTACAGCGGGAGCGA
  GGAGGAGGAGGAGGAAGTGCCTGAGCAGGAGGGAGAGCCAAGTTCCATCGTCAATGTGCCTGGAGAGTCA
  ACTCTGCGACGTGATTTCCTGAGACTGCAGCAGGAGAACAAGGAGCGGTCTGAGGCTCTGCGGAGACAGC
  AGCTTCTGCAGGAGCAGCAGCTCCGGGAGCAGGAGGAGTATAAGAGGCAGCTGCTGGCTGAGAGGCAGAA
  GCGGATTGAACAGCAGAAAGAACAGAGGAGGCGGCTGGAAGAGCAACAAAGAAGAGAACGGGAAGCCAGG
  AGGCAGCAGGAGCGTGAGCAGCGGCGGCGTGAACAAGAGGAGAAGAGGCGTCTCGAGGAACTGGAAAGGC
  GGCGTAAAGAAGAGGAAGAGAGGAGACGGGCAGAAGAGGAGAAGAGGAGAGTGGAGAGGGAACAGGAGTA
  CATCAGGCGGCAGCTAGAGGAGGAGCAGCGGCACCTGGAGATCCTGCAGCAGCAGCTGCTCCAGGAGCAG
  GCCATGTTACTGCACGACCACAGGAGGCCGCACGCACAGCAGCAGCCGCCGCCCCCGCAGCAGCAGGACA
  GGAGCAAACCGAGCTTTCATGCTCCAGAGCCCAAGCCTCACTATGACCCTGCTGACAGAGCTCGGGAGGT
  GGAAGATAGATTTAGAAAGACTAACCACAGCTCCCCTGAGGCCCAGGCTAAGCAAACGGGCAGAGGACTG
  GAGCCACCCGTGCCTTCCCGGTCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCACCCTGCCCTGC
  AGAGACCAGCGGAGCCACAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGTCTCCCCTGTCTC
  GAGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTCGCACACCACCATCTCCGCTCTCAGGACCCATGT
  CCACCTTCCCGCAGTGAGGGGCTCAGTCAGAGCTCTGACTCTAAGTCGGAGGTGCCCGAGCCCACCCAAA
  AGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCCGTGAGAACGACCTCTCGTTCCCC
  TGTCCTCTCCCGTCGGGATTCCCCACTGCAGGGCGGTGGACAGCAGAATAGCCAAGCAGGACAGAGAAAT
  TCCACCAGCAGTATTGAGCCCCGGCTTCTTTGGGAGAGAGTGGAAAAGCTGGTCCCCAGGCCAGGCAGTG
  GCAGCTCTTCAGGGTCCAGCAACTCAGGATCCCAGCCTGGCTCCCATCCTGGGTCTCAGAGCGGCTCCGG
  TGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGTTCTCCATCGCCACGCCAGGAAAGTGCAGCC
  AAAAAGCCTGACGATAAGAAAGAAGTATTCCGGCCTCTCAAGCCTGCGGGAGAAGTGGACTTGACTGCGT
  TGGCCAAAGAGCTTCGAGCAGTGGAAGATGTTCGGCCACCTCACAAAGTAACAGACTACTCATCCTCCAG
  TGAGGAGTCTGGGACCACAGATGAGGAGGAGGAGGACGTGGAACAGGAGGGGGCTGATGATTCTACCTCG
  GGACCAGAAGACACCAGAGCAGCGTCATCTCCGAACCTGAGCAACGGTGAAACAGAATCTGTGAAAACAA
  TGATCGTTCATGATGATGTAGAAAGTGAGCCAGCCATGACCCCGTCCAAGGAGGGCACCCTCATCGTCCG
  CCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGAC
  CCCAGGTTACTACAGATCTCTCCATCTAGTGGGACGACAGTGACTTCCGTGGTGGGATTTTCCTGTGATG
  GGCTGAGACCAGAAGCCATAAGGCAAGATCCTACTCGGAAAGGCTCAGTGGTCAATGTGAACCCCACGAA
  CACTAGGCCACAGAGTGATACCCCCGAGATTCGTAAATACAAGAAGAGATTTAACTCAGAGATCCTGTGT
  GCTGCCTTATGGGGAGTGAACTTGCTGGTTGGTACAGAGAGCGGTCTGATGCTCTTGGACAGAAGTGGCC
  AAGGGAAAGTATACCCTCTGATCAGCCGAAGACGGTTCCAGCAAATGGATGTGCTCGAAGGCCTAAATGT
  CCTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTATCCTGGCTAAGAAATAAGATT
  CTTCATAATGATCCAGAGGTTGAGAAGAAGCAGGGGTGGACCACTGTGGGCGACTTGGAAGGGTGCGTGC
  ACTATAAAGTCGTAAAATATGAAAGAATCAAGTTTCTGGTAATTGCTTTGAAGAGTTCTGTGGAAGTCTA
  TGCATGGGCACCGAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAACTGCTACATAAGCCA
  TTACTGGTGGATCTCACTGTGGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCGGGATTCC
  ATGCTGTTGATGTGGATTCAGGCTCAGTCTATGACATTTATCTACCAACACATATTCAGTGTAGCATCAA
  ACCCCATGCAATCATTATCCTCCCCAACACGGATGGGATGGAGCTGCTGGTGTGCTACGAAGACGAGGGG
  GTTTACGTTAACACGTACGGAAGGATCACCAAGGACGTGGTTCTGCAGTGGGGAGAGATGCCGACATCTG
  TAGCATATATCCGGTCGAATCAGACAATGGGCTGGGGAGAGAAGGCAATAGAGATACGATCTGTGGAAAC
  TGGTCACTTGGATGGTGTATTTATGCACAAAAGGGCTCAGAGACTAAAGTTCCTGTGTGAACGCAATGAC
  AAGGTGTTCTTTGCCTCCGTCCGCTCTGGTGGCAGCAGCCAGGTCTATTTCATGACCTTAGGCAGGACTT
  CTCTTCTGAGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231754 representing NM_001252200
  Red=Cloning site Green=Tags(s)
  
  MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEI
  NMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRG
  LAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY
  DYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPST
  EQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGES
  TLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREAR
  RQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQ
  AMLLHDHRRPHAQQQPPPPQQQDRSKPSFHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGL
  EPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPC
  PPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGGGQQNSQAGQRN
  STSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSPRQESAA
  KKPDDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTS
  GPEDTRAASSPNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFID
  PRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILC
  AALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKI
  LHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELLHKP
  LLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEG
  VYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERND
  KVFFASVRSGGSSQVYFMTLGRTSLLSW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252200
• ORF Size: 3864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252200.1, NP_001239129.1
• RefSeq Size: 5782 bp
• RefSeq ORF: 3867 bp
• Locus ID: 26921
• UniProt ID: P97820
• Cytogenetics: 1 B
• MW: 147.2 kDa
• Gene Summary: Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322 (By similarity).[UniProtKB/Swiss-Prot Function]