Hipk1 (NM_001301304) Mouse Tagged ORF Clone

CAT#: MR231688

Hipk1 (myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 1 (Hipk1), transcript variant 2

Product Images

Circular map for MR231688

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hipk1
• Synonyms: 1110062K04Rik; Myak
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231688 representing NM_001301304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACAGCTGCAGGTGTTTTCGCCCCCATCAGTGTCGTCGAGTGCCTTCTGCAGTGCAAAGAAAC
  TGAAAATAGAGCCCTCTGGCTGGGATGTTTCAGGACAGAGCAGCAACGACAAATACTATACCCACAGCAA
  AACCCTCCCAGCTACACAAGGGCAAGCCAGCTCCTCTCACCAGGTAGCAAATTTCAATCTTCCTGCTTAC
  GACCAGGGCCTCCTTCTCCCAGCTCCTGCCGTGGAGCATATTGTGGTAACAGCTGCTGATAGCTCAGGCA
  GCGCCGCTACAGCAACCTTCCAAAGCAGCCAGACCCTGACTCACAGGAGCAACGTTTCTTTGCTTGAGCC
  ATATCAAAAATGTGGATTGAAGAGAAAGAGTGAGGAAGTGGAGAGCAACGGTAGCGTGCAGATCATAGAA
  GAACACCCCCCTCTCATGCTGCAGAACAGAACCGTGGTGGGTGCTGCTGCCACGACCACCACTGTGACCA
  CCAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGAC
  CAACAGCTATGAAGTCCTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCAAAGTGCTGGAAGCGG
  AGCACCAAGGAAATTGTGGCCATTAAGATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTG
  AAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAACTTTGTCCGTTCTTATGAGTG
  TTTTCAGCACAAGAATCATACCTGCCTTGTGTTTGAGATGTTGGAGCAGAACTTGTACGATTTTCTAAAG
  CAGAACAAGTTTAGCCCACTGCCACTCAAGTACATAAGACCAATCTTGCAGCAGGTGGCCACAGCCCTGA
  TGAAGCTGAAGAGTCTTGGTCTGATTCATGCTGACCTTAAACCTGAAAACATAATGCTAGTCGATCCAGT
  TCGCCAACCCTACCGAGTGAAGGTCATTGACTTTGGTTCTGCTAGTCATGTTTCCAAAGCCGTGTGTTCA
  ACCTACCTGCAATCACGCTACTACAGAGCTCCTGAAATTATCCTTGGATTACCATTCTGTGAAGCTATTG
  ACATGTGGTCACTGGGCTGTGTAATAGCTGAGCTGTTCCTGGGATGGCCTCTTTATCCTGGTGCTTCAGA
  ATACGATCAGATTCGCTATATTTCACAAACACAAGGCCTGCCAGCTGAGTATCTTCTCAGTGCCGGAACA
  AAAACAACCAGGTTTTTTAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCTGAAG
  AACATGAATTGGAAACTGGAATAAAGTCAAAAGAAGCTCGGAAGTACATTTTTAACTGTTTAGATGACAT
  GGCTCAGGTAAATATGTCTACAGACTTAGAGGGGACAGATATGTTAGCAGAGAAAGCAGATCGGAGAGAG
  TATATTGATCTTCTAAAGAAAATGCTGACGATTGATGCAGATAAGAGAATCACGCCTCTGAAGACTCTTA
  ACCACCAATTTGTGACGATGAGTCACCTCCTGGACTTTCCTCACAGCAGCCACGTTAAGTCCTGTTTCCA
  GAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGACACAGTGAGTCAGATCAAGAGTCCCTTCACT
  ACACATGTCGCTCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAACCAGCTCAACACAGTGCACA
  ATCAGGCCAGTGTTCTAGCTTCCAGCTCTACTGCAGCAGCAGCTACCCTTTCTCTGGCTAATTCAGATGT
  CTCGCTGCTAAACTACCAATCGGCTTTGTACCCATCGTCGGCAGCGCCAGTTCCTGGAGTTGCCCAGCAG
  GGTGTTTCCTTACAACCTGGAACCACCCAGATCTGCACTCAGACAGATCCATTCCAGCAAACATTTATAG
  TATGCCCACCTGCTTTTCAGACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGA
  TAATGCTGTGCCAATTGTACCCCAGGCGCCTGCTGCTCAGCCGCTGCAGATCCAGTCAGGAGTACTCACA
  CAGGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACGCCGCAGTATG
  CGGTGCCCTTTACCCTGAGCTGCGCAGCAGGCCGGCCGGCGCTGGTTGAACAGACTGCTGCTGTACTGCA
  AGCCTGGCCTGGAGGAACCCAACAAATTCTCCTGCCTTCAGCCTGGCAGCAGCTGCCCGGGGTAGCTCTG
  CACAACTCTGTCCAGCCTGCTGCAGTGATTCCAGAGGCCATGGGGAGCAGCCAACAGCTAGCTGACTGGA
  GGAATGCCCACTCTCATGGCAACCAGTACAGCACTATTATGCAGCAGCCATCTTTGCTGACCAACCATGT
  GACCTTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAGTCTAGTTCC
  CTCCCTTCAAAGAAGAATAAGCAGTCTGCTCCAGTTTCATCCAAATCCTCTCTGGAAGTCCTGCCTTCTC
  AAGTTTATTCTCTGGTTGGGAGTAGTCCTCTTCGTACCACATCTTCTTATAATTCCCTAGTTCCTGTCCA
  AGACCAGCATCAGCCAATCATCATTCCAGATACCCCCAGCCCTCCTGTGAGTGTCATCACTATCCGTAGT
  GACACTGATGAAGAAGAGGACAACAAATACAAGCCCAATAGCTCGAGCCTGAAGGCGAGGTCTAATGTCA
  TCAGTTATGTCACTGTCAATGATTCTCCAGACTCTGACTCCTCCCTGAGCAGCCCACATCCCACAGACAC
  TCTGAGTGCTCTGCGGGGCAACAGTGGGACCCTTCTGGAGGGACCTGGCAGACCTGCAGCAGATGGCATT
  GGCACCCGTACTATCATTGTGCCTCCTTTGAAAACACAGCTTGGCGACTGCACTGTAGCAACACAGGCCT
  CAGGTCTCCTTAGCAGTAAGACCAAGCCAGTGGCCTCAGTGAGTGGGCAGTCATCTGGATGCTGTATCAC
  TCCCACGGGGTACCGGGCTCAGCGAGGGGGAGCCAGCGCGGTGCAGCCACTCAACCTTAGCCAGAACCAG
  CAGTCATCGTCAGCTTCAACCTCGCAGGAAAGAAGCAGCAACCCTGCTCCCCGCAGACAGCAGGCATTTG
  TGGCCCCGCTCTCCCAAGCCCCCTACGCCTTCCAGCATGGCAGCCCACTGCACTCGACGGGGCACCCACA
  CTTGGCCCCAGCCCCTGCTCACCTGCCAAGCCAGCCTCACCTGTATACGTACGCTGCCCCCACTTCTGCT
  GCTGCATTGGGCTCCACCAGTTCCATTGCTCATCTGTTCTCCCCCCAGGGTTCCTCAAGGCATGCTGCAG
  CTTATACCACACACCCTAGCACTCTGGTGCATCAGGTTCCTGTCAGTGTCGGGCCCAGCCTCCTCACTTC
  TGCCAGTGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACTCAGTCCTACATCGGGTCTTCCCGAGGC
  TCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGTCAGTATTCTTACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231688 representing NM_001301304
  Red=Cloning site Green=Tags(s)
  
  MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVANFNLPAY
  DQGLLLPAPAVEHIVVTAADSSGSAATATFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVESNGSVQIIE
  EHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKR
  STKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLK
  QNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCS
  TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGT
  KTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRRE
  YIDLLKKMLTIDADKRITPLKTLNHQFVTMSHLLDFPHSSHVKSCFQNMEICKRRVHMYDTVSQIKSPFT
  THVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQ
  GVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLT
  QGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSAWQQLPGVAL
  HNSVQPAAVIPEAMGSSQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSS
  LPSKKNKQSAPVSSKSSLEVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRS
  DTDEEEDNKYKPNSSSLKARSNVISYVTVNDSPDSDSSLSSPHPTDTLSALRGNSGTLLEGPGRPAADGI
  GTRTIIVPPLKTQLGDCTVATQASGLLSSKTKPVASVSGQSSGCCITPTGYRAQRGGASAVQPLNLSQNQ
  QSSSASTSQERSSNPAPRRQQAFVAPLSQAPYAFQHGSPLHSTGHPHLAPAPAHLPSQPHLYTYAAPTSA
  AALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRG
  STIYTGYPLSPTKISQYSYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301304
• ORF Size: 3630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301304.1, NP_001288233.1
• RefSeq Size: 7939 bp
• RefSeq ORF: 3633 bp
• Locus ID: 15257
• UniProt ID: O88904
• Cytogenetics: 3 F2.2
• MW: 130.7 kDa
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.[UniProtKB/Swiss-Prot Function]