Hps5 (NM_001005248) Mouse Tagged ORF Clone

CAT#: MR231606

Hps5 (myc-DDK-tagged) - Mouse Hermansky-Pudlak syndrome 5 homolog (human) (Hps5), transcript variant 2

Product Images

Circular map for MR231606

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hps5
• Synonyms: AI646796; AL022647; C85120; haze; hz; maroon; mr; ru-2; ru2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231606 representing NM_001005248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTTGTGCCAGTGATACCAGAAGCCTACAGCCATGTTCTTGCAGAGTTTGAGTCCTTGGATCCGT
  TACTCACAGCCCTGAGGCTGGACTCCAGTCGTCTGAGGTGTACCAGCATAGCTGTGTCTCGGAAATGGTT
  GGCGTTAGGCAGCACAGGAGGAGGACTCAATCTCATTCAGAAAGATGGCTGGAAGCAAAGGCTGTTTCTC
  TCTCACCGGGAAGGGGCAATCTCTCAGATTGCCTGCTGCTCTCATGATGATGATTATGTTGCTGTAGCTA
  CCAGTCAAGGTCTTGTAGTTGTTTGGGAATTAAATCAAGAGCGTCGTGGGAAGCCAGAACGAATTCATGT
  GTCCTCAGAACACAAAGGCCGAAAAGTTACTGCCCTCTGTTGGGACACGGCTGTTCTGAGAGTCTTTGTC
  GGTGACCATGTGGGCAAAGTATCTGCCATCAAACTCAACACTTTGAAACAAGCAAAAGCAGCTGCTGCCT
  TTGTGATGTTTCCTGTGCAGACAGTACTAACAGTGGACTCGTGTGTCGTCCAGTTGGATTACTTGGATGG
  AAGATTACTTGTGTCTTCACTGACTCGATCCTTCTTGTGTGACACCGAGAGGGAAAAGTTTTGGAAAATC
  GGAAATAAGGAAAGACATGGAGAATATGGGGCTTGTTTCTTCCCCGGGCGGTGTGCTGGGGGCCAGCAGC
  CTGTGATCTACTGCGCCCGCCCAGGCTCCCGGATGTGGGAAGTGAACTTTGACGGGGAAGTGCTCAGTAC
  ACACCAGTTCAAGAAGCTCCTGTCAATGCCACCCCTCCCTGTGATCACTGCAAGATCAGAGCCTCAGTAT
  GATCACACAGTTGGATCCTCCCAGTCTTTGGCATTCCCCAAACTCTTGCATCTTAGTGAACACTGTGTGC
  TGACTTGGACAGAAAAGGGAATTTATATTTTCATTCCTCAGAATGTTCAAGTTCTTCTTTGGAGTGAAGT
  CAAGGATATTCAGGATGTAGCTGTCTACAAGAATGAATTGTTCTGTTTACACTTTAATGGAAAAATCTCA
  CATCTTTCCCTGTTGTCTGTGGAGCGCTGTGTAGAACGCCTGTTAAGGAGAGGCCTGTGGGACCTGGCTG
  CTCGCACATGCTGTCTCTTCCAAAATTCTATTATTACCAGCAGAGCAAGAAAAACATTGACTGCAGATAA
  GTTGGAGCATTTGAAGTCTCAGCTGGACCTTACAGCCTGCAGTGAACTAATTTCTCAGCTGGACGACTTG
  ATCTTAAGGTTCGAGCCTTTGGAGTCGGCGTGCAGCAGCAGAAGAAGCTCCATCTCATCGCATGAAAGTT
  TTAGCATCTTGGACTCTGGTATTTATCGTATCATCAGTAGTAGAAGAGGCAGTCAGTCCGACGAAGATTC
  TTGTTCCCTTCACAGCCAGACCTTCTCAGAAGATGAAAGGCTTAAAGAATTTGCCTCACACCAGGAAGAG
  GAGCAGCCAGAGCAGGGCTGTGGTGCCAATAGGAATGAAGAGAGTGCTTCCCACAGTCCAGTGATGTCTG
  AGGTAGATAAGAGTGAAGCTTTTCTGCCCTTCAGCATTGCACTGCCATTTCGTTCTCCATCTCCCCTCGT
  GTCTCTTCAGGCTGTTAAAGACAGTGTTTCTAGCTTTGTGCGTAAAACTACTGAGAAGATTGGCACCCTT
  CACGGAAGCCCCGAGCTGAAAGAGCCTTTTGAATCCAAGGATGCTGACCGAGCACATGAAGAGGAAGTGA
  GTGCAGTCACCTGCCCTCTGGAGGAGGACACTGAGGAGAAAGAAATTCATCAACCTCCTAAAGAAGACAG
  GCTTCAAGAACTCACAGCAGCGACAGCAGAAGCAATGACCAAGTTATTGGACCCTCTGGTTCTGTTTGAG
  CCCAAGGTGCTAAGAATGGTTTTACTTGAGTGGCTTTCACAGTTAGAGAAAACATTTGCCATGAAAGACT
  TCCCCGGCATTTCCAATACCAGCAGTCCAACCGTGAAATCAAACTTGGGTGCACACCTCCTCGGTGAGAC
  AGAAAAGAGAGTATTAGATGAAGAGAGTGGAGAAGGAAGAAGGGTCTCTTTAGTCACTGAAGAAGCTGGA
  GGTCAGATAACCTGTGACCCTGTAAGCAACCTCAGTGAGCCCTCGGCTGACCGTTTTCGAGTATGCTCTC
  CATACGCCATCACAAACAGCCTTCAGAGGGACCTGGCGGAATTGACAACGTTGTGCTTGGAACTGAATGT
  GTTGACTTCAGCAATGGAAAGCGTAGGTGGGCATGTGGACCGGGCTTCACAGCAGCTCTCTCCAGAAATC
  CTGGCTTGTCGTTTCCTAAAGAAGTACTTTTTTCTTCTGGACTTGAAAAGAGCAAAGGAGAGTATCAAGC
  TCACTTACGACAGCCCTTGCGTTTGGGACACTTTTGTCGAAGGACTAAAAGAAATGGCAAGATCTAATCC
  TGCCTACACGGAGCTGGAAGAAGGAGACCTGCCCACGGGGTTACAGTTACTGGATGGCTCCGTCCCTTCT
  GATAGTCCCTTGTTGATTGCATTTGCCACCCGGTTGTATGATAGATTTGGGGAGTCAGCCCTTCGAGCTT
  GCATCAAGTTTTATCCGTCCATTTCGCCCTCCGACATCGCACAGCTTTGCCGTCATCATCCTGCTCAGTT
  CTTGGCCTATCTAGACAGTCTGGTGAAGTCCAGGCCGGAAGACCAGTGGCCATCCTTCCTTGAGTTCCTT
  CTACAACCAGAGTCTTTAAGACTTGAATGGCTGCTTTTGGCAGTGTCCCATGATGCTCCACCAAGCACAA
  GTACAGTGGATGATGAAGGGCACCCCAGGCCCCATTCACACTTGCTTTCTTGGGGTTATAGTCAGCTGAT
  TCTTCTTCTTATTAAACTTCCTGCAGACTTTACAACCAAAGAGAAAATGACTGACATCTGTAGGTCTTAC
  GGTTTCTGGCCTGGATATCTTACCCTCTGTCTGGAGCTGGAGAGGAGGAGGGAGGCCTTCACCAACATTG
  TGTATCTGAATGACATAAGCCTGATGGAAGGGGACAATGGGTGGATCCCTGAGACCTTGGAGGAATGGAA
  GCTTCTCCTACATCTACTACAGACCAAGAGCACAAGGCCAGCTCCCCAGGAGTCACTAAACGGGAGCCTC
  AGTGATGGGCCAGCCCCTATCAATGTGGAGAATGTGGCCCTCCTGTTAGCTAAGGCCATGGGCCCAGATC
  GGGCCTGGTCACTGCTACAGGAGTGTGGTCTGGCTCTTGAGTTGTCAGAAAAGTTCACCAGAACCTGTGA
  TATCCTGAGGATTGCTGAGAGAAGGCAGAGAGCGCTGATACAAGGCATGCTTGAGAAGTGTGACCGCTTC
  CTCTGGTCGCAGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231606 representing NM_001005248
  Red=Cloning site Green=Tags(s)
  
  MTFVPVIPEAYSHVLAEFESLDPLLTALRLDSSRLRCTSIAVSRKWLALGSTGGGLNLIQKDGWKQRLFL
  SHREGAISQIACCSHDDDYVAVATSQGLVVVWELNQERRGKPERIHVSSEHKGRKVTALCWDTAVLRVFV
  GDHVGKVSAIKLNTLKQAKAAAAFVMFPVQTVLTVDSCVVQLDYLDGRLLVSSLTRSFLCDTEREKFWKI
  GNKERHGEYGACFFPGRCAGGQQPVIYCARPGSRMWEVNFDGEVLSTHQFKKLLSMPPLPVITARSEPQY
  DHTVGSSQSLAFPKLLHLSEHCVLTWTEKGIYIFIPQNVQVLLWSEVKDIQDVAVYKNELFCLHFNGKIS
  HLSLLSVERCVERLLRRGLWDLAARTCCLFQNSIITSRARKTLTADKLEHLKSQLDLTACSELISQLDDL
  ILRFEPLESACSSRRSSISSHESFSILDSGIYRIISSRRGSQSDEDSCSLHSQTFSEDERLKEFASHQEE
  EQPEQGCGANRNEESASHSPVMSEVDKSEAFLPFSIALPFRSPSPLVSLQAVKDSVSSFVRKTTEKIGTL
  HGSPELKEPFESKDADRAHEEEVSAVTCPLEEDTEEKEIHQPPKEDRLQELTAATAEAMTKLLDPLVLFE
  PKVLRMVLLEWLSQLEKTFAMKDFPGISNTSSPTVKSNLGAHLLGETEKRVLDEESGEGRRVSLVTEEAG
  GQITCDPVSNLSEPSADRFRVCSPYAITNSLQRDLAELTTLCLELNVLTSAMESVGGHVDRASQQLSPEI
  LACRFLKKYFFLLDLKRAKESIKLTYDSPCVWDTFVEGLKEMARSNPAYTELEEGDLPTGLQLLDGSVPS
  DSPLLIAFATRLYDRFGESALRACIKFYPSISPSDIAQLCRHHPAQFLAYLDSLVKSRPEDQWPSFLEFL
  LQPESLRLEWLLLAVSHDAPPSTSTVDDEGHPRPHSHLLSWGYSQLILLLIKLPADFTTKEKMTDICRSY
  GFWPGYLTLCLELERRREAFTNIVYLNDISLMEGDNGWIPETLEEWKLLLHLLQTKSTRPAPQESLNGSL
  SDGPAPINVENVALLLAKAMGPDRAWSLLQECGLALELSEKFTRTCDILRIAERRQRALIQGMLEKCDRF
  LWSQQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005248
• ORF Size: 3378 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005248.2, NP_001005248.2
• RefSeq Size: 4804 bp
• RefSeq ORF: 3381 bp
• Locus ID: 246694
• UniProt ID: P59438
• Cytogenetics: 7 30.56 cM
• MW: 126.3 kDa
• Gene Summary: May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. Regulates intracellular vesicular trafficking in fibroblasts. May be involved in the regulation of general functions of integrins.[UniProtKB/Swiss-Prot Function]