Prrt3 (NM_001289699) Mouse Tagged ORF Clone

CAT#: MR231447

Prrt3 (myc-DDK-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3), transcript variant 1

Product Images

Circular map for MR231447

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prrt3
• Synonyms: 6330505P20; B230206N24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231447 representing NM_001289699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCAGCCCACAGGCCTGCACATCTCCTCTTCTGCTGTTGCTGCTGCCATGCCTGGGGGCTGGCC
  CTGCCTTGGGCAGAGGCCTTCCCAGGCCCCTGGAGAACTCAGAACCTCACATGATCCCATCAGAGTCACA
  GACCTTTGACCTCTTCTGGGAGAAGCTGAGAAATGAGAGTTCCTGGCACAGCGGTGACCCTCAGGCCCGT
  GCTGAGGGGCCCAAGAAGCCCGCAGACCCTTACCTAGGACCAGCCCTGCATGGGCCCAAGGCAGCCCCTG
  GGGTCCAAGGTGAAAGACTACTAAGAGCTGATGACCTCCAGCTGGCCCGAGCATTCACCTCCCAAGGCTG
  GACAGGACCTCCTGACTCACAGGAGCTTCTGGAGCCTGAGGCACCAGAACCCCACCCTGTGCGAGCACCT
  CGTCTCACTCTTGTCACTACAACTCCCAGCTCCCTACTCTCGGCAGCCATACTTTCCACTGCCTCCCAGA
  AGCCTGGAGGCACAGCAGGGCAGCAGCCAGCTAGGAACGAGGAGCTAATAATGGTCAAAGCCGAGACCCA
  CATCACACAGGCTTCCCCCTGGGACTTCCAGGGCTCTTCCCACACTCCGGTACCCGAGACAGATGCTGTG
  AGGACGCTGGTGTTGGGGAAGCAGGGGGGACATGAACAGGGCTTCCAGGAGGCTGTCCAGGGTCCCCTAC
  TCACCCAGCAGGATCCAGTAGTCCCTGGGGTCGGCTCAACACCCCCAGTTAAAGTGGAATCCACCCCAGA
  GCCTGGAGCCCAGCTCGACCTGGCATTGGTCAGAAGCCTTCCTCTTCCTGAGGGGCTGCCAGCTGAACCC
  CCCAAAACTGGAGCTGGGGATACCTGGGAAGTCAGCTCTCTGGGACCCCAACCAGAACAGACCGATCTCC
  TTGGAGGACAAGATTCCCCAGCACCCCAGCCTATACCACCTTCAGCCTCAGACACTTCTGATGGGCACCT
  AAGACCAGCAGTATCCTCGCTGAATGGAGCCGACCCCATCTCCCCACAACGTGTGAGAGGAGCGATGGAG
  GCCCCAGGAACCCCCAAGTCCTTCATCCCTGACCTTCCAAATTCTGCTCAAGCTGCAAATGGAACAGAGA
  GCCCTGTGAGGGCCCTGCAGCCAGATGAAGCCGAGGATTGGCCGGGTCGCCCACAAAGCCACCCTCCAGC
  ACCCCCTGTCCAGGCCCCCTCGACATCTCGGCGTGGTCTCGTTAGAGTCACCACACAGAGAGCTCTGGGC
  CAGCCACTCCCTCCGGAGCCCTCAGCCAGCTCCATAGTCCCCATCCCAGCTTCTAGCCCCCCAGCCAATG
  CCACCGCACCTCCCCTGCGTTGGGGACCCCTTCGGCGGGTGCTGAGTTTCTCCTGGGAGCTTCATGTGTA
  TGGGGTGGGGGTGCTTTTCCTGCTTCCAGCATTATTGGCATTGGTCACAGTGGCAGCCGCCCTAGCGGGA
  CCCCGATTAGCACTAGTGGCTGCCGCGCTGGTATTGGTGGCTTCAGGGCTGCGATCTGCCTACATGCTCA
  CGGATCCTTATGGCTCTCAGGCGCGACTGGGAGTACGTGCAGGTCTGGTGCTATACAACTTGCCCTTCCC
  CTTGCTACTCACTGCACTGGCAGCACTCACCCTGCTGGGCTTGGGTGCTGGTCTGCCACAGCCTCTGCAG
  AAGCCGCTGCTGCTGGGAATTGTGGCACCGGTGCATGGCACATGTTTGCTGGCTACAGACCTGTTCTCCA
  CCAGCCCTGTGCTCAACCTTCTAACACAAGGCTTATCTTGCGCCTGGGGCGCCTCAGTGGCTCTGGGCAC
  ACTCTGCCTGTGCCGTCGACGCCTGCTGGAAGGCCCTCGGGGCTGGGATGCCAGTCCAGGGCCCCGGCTG
  TTGGCTGTGGCGGGCTCACTGGGGCTGTTGGCCAGTGGTCTGCAGTTGGCGGCCTCTCTCTGGCTGTACC
  CAGGGCCTGGCCGGGAGGGTCGCTTCTCCTGGGCTTGGTGGGGCGTGCACTTCTGGCTGCGCCTGCTGGA
  ATTGACCTGGGCACTGGCCCTGGCGCTAGCTGCCCTGGCTGCCACTCGTCCCAGGCCGCCCACGGAGCAC
  GCTTGCTGGGCTAAGCTGCTGCGTCTGGCGTGCCCCGCGCCTACAGGGAAGAGCGAAGTTCCCGAGAGAC
  CCAATAACTGCTATGCGGGGCCCAGTGGCCTGGGCACAGGCGGCCTGGACATCAGCAAAAGTCTCATCCG
  CAACGCTGCTGGGGAAGCTGGGCTTCCTGTCACTCCTGGTTCTGGACCCTGGGGTTCGGCTGCATCGCTG
  GGTCGCGGTCGTCCAGGTGGCCAGAGGATGTCCCGGGGCAGTGTGGGGCCCGCACCGTCGCTGAGCGAGC
  TGGATCTGCGGCCACCGTCACCTATCAATTTGAGCCGCAGCATCGACGCCGCGCTCTTCCGGGAGCACTT
  GGTTCGAGAGAGTGTGTTCCAACGCTGTGGTCTCCGTGGCTTGGCCTCCTCGCCGACAGGGGGCGCCCTG
  CGGCCTCGCCGCGGCAGCCAGCCCGATGCCGAGCTCGACGGCGCGGGCACATCATTGCTCCGAGGCCGCT
  GCCGCTCGCTCACCGAGGTGTGCTTGCGCACGTCGCTCCCGCAGCACGTGATGGAGCCGCCCGTCGGCGC
  GGCGGCCGCGGGGACTTCGGGCAGCTCCCTGGACAGTTTCTCCAAGGGCTCGCTGAAGATCAGCTGGAAC
  CCGTGGCGTCACGGGCTGTCATCCGTGGACAGCCTGCCCCTAGATGAACTGCCCAGCACCGTGCAGCTGC
  TGCCACCACCTACCCCAGTCCCTGCTCCTGCCCGGGCGGGGGAGCCTCAGGGCGAGGGCCAGTCGCGTTG
  CAAGTCCAGCGAGTCTCACAGCGCCTCTAGTGACACTATAGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231447 representing NM_001289699
  Red=Cloning site Green=Tags(s)
  
  MAPSPQACTSPLLLLLLPCLGAGPALGRGLPRPLENSEPHMIPSESQTFDLFWEKLRNESSWHSGDPQAR
  AEGPKKPADPYLGPALHGPKAAPGVQGERLLRADDLQLARAFTSQGWTGPPDSQELLEPEAPEPHPVRAP
  RLTLVTTTPSSLLSAAILSTASQKPGGTAGQQPARNEELIMVKAETHITQASPWDFQGSSHTPVPETDAV
  RTLVLGKQGGHEQGFQEAVQGPLLTQQDPVVPGVGSTPPVKVESTPEPGAQLDLALVRSLPLPEGLPAEP
  PKTGAGDTWEVSSLGPQPEQTDLLGGQDSPAPQPIPPSASDTSDGHLRPAVSSLNGADPISPQRVRGAME
  APGTPKSFIPDLPNSAQAANGTESPVRALQPDEAEDWPGRPQSHPPAPPVQAPSTSRRGLVRVTTQRALG
  QPLPPEPSASSIVPIPASSPPANATAPPLRWGPLRRVLSFSWELHVYGVGVLFLLPALLALVTVAAALAG
  PRLALVAAALVLVASGLRSAYMLTDPYGSQARLGVRAGLVLYNLPFPLLLTALAALTLLGLGAGLPQPLQ
  KPLLLGIVAPVHGTCLLATDLFSTSPVLNLLTQGLSCAWGASVALGTLCLCRRRLLEGPRGWDASPGPRL
  LAVAGSLGLLASGLQLAASLWLYPGPGREGRFSWAWWGVHFWLRLLELTWALALALAALAATRPRPPTEH
  ACWAKLLRLACPAPTGKSEVPERPNNCYAGPSGLGTGGLDISKSLIRNAAGEAGLPVTPGSGPWGSAASL
  GRGRPGGQRMSRGSVGPAPSLSELDLRPPSPINLSRSIDAALFREHLVRESVFQRCGLRGLASSPTGGAL
  RPRRGSQPDAELDGAGTSLLRGRCRSLTEVCLRTSLPQHVMEPPVGAAAAGTSGSSLDSFSKGSLKISWN
  PWRHGLSSVDSLPLDELPSTVQLLPPPTPVPAPARAGEPQGEGQSRCKSSESHSASSDTIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289699
• ORF Size: 2916 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289699.1, NP_001276628.1
• RefSeq Size: 3789 bp
• RefSeq ORF: 2919 bp
• Locus ID: 210673
• Cytogenetics: 6 E3
• MW: 101.7 kDa